Strain Fitness in Escherichia coli BW25113 around b1944

Experiment: 6 hr rep2_HD100_10fold_dilution_grewON

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfliK and fliL are separated by 104 nucleotidesfliL and fliM are separated by 4 nucleotides b1943: fliK - flagellar hook-length control protein (NCBI), at 2,016,410 to 2,017,537 fliK b1944: fliL - flagellar basal body-associated protein FliL (NCBI), at 2,017,642 to 2,018,106 fliL b1945: fliM - flagellar motor switch protein M (NCBI), at 2,018,111 to 2,019,115 fliM Position (kb) 2017 2018 2019Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 2016.658 kb on - strand, within fliKat 2016.927 kb on - strand, within fliKat 2016.957 kb on - strand, within fliKat 2017.027 kb on + strand, within fliKat 2017.068 kb on - strand, within fliKat 2017.076 kb on + strand, within fliKat 2017.076 kb on + strand, within fliKat 2017.080 kb on - strand, within fliKat 2017.116 kb on + strand, within fliKat 2017.182 kb on + strand, within fliKat 2017.207 kb on + strand, within fliKat 2017.207 kb on + strand, within fliKat 2017.267 kb on + strand, within fliKat 2017.323 kb on + strand, within fliKat 2017.488 kb on + strandat 2017.757 kb on + strand, within fliLat 2017.757 kb on + strand, within fliLat 2017.797 kb on - strand, within fliLat 2017.813 kb on + strand, within fliLat 2017.947 kb on - strand, within fliLat 2017.972 kb on + strand, within fliLat 2018.029 kb on - strand, within fliLat 2018.038 kb on - strand, within fliLat 2018.038 kb on - strand, within fliLat 2018.299 kb on + strand, within fliMat 2018.411 kb on + strand, within fliMat 2018.420 kb on - strand, within fliMat 2018.466 kb on + strand, within fliMat 2018.466 kb on + strand, within fliMat 2018.608 kb on - strand, within fliMat 2018.608 kb on - strand, within fliMat 2018.610 kb on - strand, within fliMat 2018.610 kb on - strand, within fliMat 2018.652 kb on - strand, within fliMat 2018.689 kb on - strand, within fliMat 2018.689 kb on - strand, within fliMat 2018.700 kb on + strand, within fliMat 2018.870 kb on + strand, within fliMat 2018.875 kb on + strand, within fliMat 2018.890 kb on - strand, within fliMat 2019.025 kb on + strandat 2019.106 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 6 hr rep2_HD100_10fold_dilution_grewON
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2,016,658 - fliK b1943 0.22 -1.6
2,016,927 - fliK b1943 0.46 -5.8
2,016,957 - fliK b1943 0.48 +1.2
2,017,027 + fliK b1943 0.55 -4.2
2,017,068 - fliK b1943 0.58 -3.9
2,017,076 + fliK b1943 0.59 -1.3
2,017,076 + fliK b1943 0.59 +1.7
2,017,080 - fliK b1943 0.59 +1.5
2,017,116 + fliK b1943 0.63 -2.9
2,017,182 + fliK b1943 0.68 -3.8
2,017,207 + fliK b1943 0.71 +0.6
2,017,207 + fliK b1943 0.71 +0.8
2,017,267 + fliK b1943 0.76 +0.1
2,017,323 + fliK b1943 0.81 +1.1
2,017,488 + -4.6
2,017,757 + fliL b1944 0.25 -0.9
2,017,757 + fliL b1944 0.25 +0.4
2,017,797 - fliL b1944 0.33 +0.6
2,017,813 + fliL b1944 0.37 -4.3
2,017,947 - fliL b1944 0.66 +0.1
2,017,972 + fliL b1944 0.71 -1.0
2,018,029 - fliL b1944 0.83 -4.6
2,018,038 - fliL b1944 0.85 -0.0
2,018,038 - fliL b1944 0.85 -3.8
2,018,299 + fliM b1945 0.19 -1.4
2,018,411 + fliM b1945 0.30 -1.3
2,018,420 - fliM b1945 0.31 -0.3
2,018,466 + fliM b1945 0.35 -0.0
2,018,466 + fliM b1945 0.35 -5.0
2,018,608 - fliM b1945 0.49 -4.0
2,018,608 - fliM b1945 0.49 +0.1
2,018,610 - fliM b1945 0.50 +1.6
2,018,610 - fliM b1945 0.50 +1.4
2,018,652 - fliM b1945 0.54 +0.5
2,018,689 - fliM b1945 0.58 +0.9
2,018,689 - fliM b1945 0.58 -0.1
2,018,700 + fliM b1945 0.59 +1.7
2,018,870 + fliM b1945 0.76 -0.4
2,018,875 + fliM b1945 0.76 -0.2
2,018,890 - fliM b1945 0.78 -0.2
2,019,025 + +0.2
2,019,106 + +1.1

Or see this region's nucleotide sequence