Experiment: 6 hr rep2_HD100_10fold_dilution_grewON
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sfcA and sra are separated by 156 nucleotides sra and b1481 are separated by 101 nucleotides b1481 and osmC are separated by 344 nucleotides osmC and ddpF are separated by 55 nucleotides ddpF and ddpD overlap by 8 nucleotides
b1479: sfcA - NAD-linked malate dehydrogenase (malic enzyme) (VIMSS), at 1,551,996 to 1,553,693
sfcA
b1480: sra - 30S ribosomal subunit protein S22 (NCBI), at 1,553,850 to 1,553,987
sra
b1481: b1481 - orf, hypothetical protein (VIMSS), at 1,554,089 to 1,554,304
b1481
b1482: osmC - osmotically inducible, stress-inducible membrane protein (NCBI), at 1,554,649 to 1,555,080
osmC
b1483: ddpF - D-ala-D-ala transporter subunit (NCBI), at 1,555,136 to 1,556,062
ddpF
b1484: ddpD - D-ala-D-ala transporter subunit (NCBI), at 1,556,055 to 1,557,041
ddpD
Position (kb)
1554
1555
1556 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 1553.700 kb on - strand at 1553.833 kb on + strand at 1553.833 kb on + strand at 1553.951 kb on + strand, within sra at 1553.951 kb on + strand, within sra at 1553.951 kb on + strand, within sra at 1554.079 kb on + strand at 1554.082 kb on - strand at 1554.097 kb on - strand at 1554.101 kb on + strand at 1554.111 kb on - strand, within b1481 at 1554.111 kb on - strand, within b1481 at 1554.123 kb on - strand, within b1481 at 1554.132 kb on + strand, within b1481 at 1554.144 kb on - strand, within b1481 at 1554.218 kb on + strand, within b1481 at 1554.224 kb on - strand, within b1481 at 1554.224 kb on - strand, within b1481 at 1554.244 kb on - strand, within b1481 at 1554.249 kb on + strand, within b1481 at 1554.257 kb on - strand, within b1481 at 1554.273 kb on + strand, within b1481 at 1554.355 kb on - strand at 1554.365 kb on + strand at 1554.407 kb on + strand at 1554.407 kb on + strand at 1554.599 kb on + strand at 1554.599 kb on + strand at 1554.601 kb on + strand at 1554.639 kb on - strand at 1554.678 kb on + strand at 1554.678 kb on - strand at 1554.684 kb on - strand at 1554.697 kb on + strand, within osmC at 1554.707 kb on + strand, within osmC at 1554.718 kb on + strand, within osmC at 1554.723 kb on - strand, within osmC at 1555.296 kb on + strand, within ddpF at 1555.384 kb on - strand, within ddpF at 1555.384 kb on - strand, within ddpF at 1555.388 kb on + strand, within ddpF at 1555.388 kb on + strand, within ddpF at 1555.544 kb on - strand, within ddpF at 1555.722 kb on + strand, within ddpF at 1555.722 kb on + strand, within ddpF at 1555.874 kb on - strand, within ddpF at 1555.874 kb on - strand, within ddpF
Per-strain Table
Position Strand Gene LocusTag Fraction 6 hr rep2_HD100_10fold_dilution_grewON remove 1,553,700 - +0.6 1,553,833 + -1.6 1,553,833 + -4.2 1,553,951 + sra b1480 0.73 -3.5 1,553,951 + sra b1480 0.73 -1.5 1,553,951 + sra b1480 0.73 -3.1 1,554,079 + -3.8 1,554,082 - -5.2 1,554,097 - +1.0 1,554,101 + +0.9 1,554,111 - b1481 b1481 0.10 +2.3 1,554,111 - b1481 b1481 0.10 -0.2 1,554,123 - b1481 b1481 0.16 +0.1 1,554,132 + b1481 b1481 0.20 -2.5 1,554,144 - b1481 b1481 0.25 -0.9 1,554,218 + b1481 b1481 0.60 +0.3 1,554,224 - b1481 b1481 0.62 +0.8 1,554,224 - b1481 b1481 0.62 -0.2 1,554,244 - b1481 b1481 0.72 +0.8 1,554,249 + b1481 b1481 0.74 -0.2 1,554,257 - b1481 b1481 0.78 -0.8 1,554,273 + b1481 b1481 0.85 -2.8 1,554,355 - +0.8 1,554,365 + -2.5 1,554,407 + +0.8 1,554,407 + +0.8 1,554,599 + +3.4 1,554,599 + -0.2 1,554,601 + +3.2 1,554,639 - -1.2 1,554,678 + +0.8 1,554,678 - -4.0 1,554,684 - -3.2 1,554,697 + osmC b1482 0.11 -4.1 1,554,707 + osmC b1482 0.13 -2.2 1,554,718 + osmC b1482 0.16 -0.8 1,554,723 - osmC b1482 0.17 -1.5 1,555,296 + ddpF b1483 0.17 +0.6 1,555,384 - ddpF b1483 0.27 -0.2 1,555,384 - ddpF b1483 0.27 -0.4 1,555,388 + ddpF b1483 0.27 -1.0 1,555,388 + ddpF b1483 0.27 +1.1 1,555,544 - ddpF b1483 0.44 +0.6 1,555,722 + ddpF b1483 0.63 -0.5 1,555,722 + ddpF b1483 0.63 +0.1 1,555,874 - ddpF b1483 0.80 +0.7 1,555,874 - ddpF b1483 0.80 +1.0
Or see this region's nucleotide sequence