Experiment: 6 hr rep2_HD100_10fold_dilution_grewON
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ycgL and ycgM are separated by 71 nucleotides ycgM and ycgN are separated by 76 nucleotides ycgN and hlyE are separated by 206 nucleotides
b1179: ycgL - conserved protein (NCBI), at 1,226,937 to 1,227,230
ycgL
b1180: ycgM - predicted isomerase/hydrolase (NCBI), at 1,227,302 to 1,227,961
ycgM
b1181: ycgN - conserved protein (RefSeq), at 1,228,038 to 1,228,499
ycgN
b1182: hlyE - hemolysin E (VIMSS), at 1,228,706 to 1,229,617
hlyE
Position (kb)
1228
1229 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1227.068 kb on + strand, within ycgL at 1227.091 kb on - strand, within ycgL at 1227.105 kb on + strand, within ycgL at 1227.145 kb on - strand, within ycgL at 1227.230 kb on + strand at 1227.295 kb on - strand at 1227.419 kb on - strand, within ycgM at 1227.550 kb on + strand, within ycgM at 1227.550 kb on + strand, within ycgM at 1227.593 kb on - strand, within ycgM at 1227.778 kb on + strand, within ycgM at 1227.885 kb on + strand at 1227.885 kb on + strand, within ycgM at 1227.937 kb on + strand at 1228.047 kb on - strand at 1228.060 kb on - strand at 1228.139 kb on + strand, within ycgN at 1228.146 kb on + strand, within ycgN at 1228.161 kb on + strand, within ycgN at 1228.255 kb on + strand, within ycgN at 1228.255 kb on + strand, within ycgN at 1228.264 kb on - strand, within ycgN at 1228.270 kb on + strand, within ycgN at 1228.376 kb on + strand, within ycgN at 1228.376 kb on + strand, within ycgN at 1228.376 kb on + strand, within ycgN at 1228.398 kb on + strand, within ycgN at 1228.409 kb on + strand, within ycgN at 1228.409 kb on + strand, within ycgN at 1228.409 kb on + strand, within ycgN at 1228.422 kb on + strand, within ycgN at 1228.422 kb on + strand, within ycgN at 1228.424 kb on + strand, within ycgN at 1228.424 kb on + strand, within ycgN at 1228.429 kb on - strand, within ycgN at 1228.449 kb on + strand, within ycgN at 1228.480 kb on + strand at 1228.550 kb on + strand at 1228.567 kb on + strand at 1228.584 kb on + strand at 1228.584 kb on + strand at 1228.728 kb on - strand at 1228.729 kb on - strand at 1228.769 kb on - strand at 1228.812 kb on + strand, within hlyE at 1229.017 kb on - strand, within hlyE at 1229.017 kb on - strand, within hlyE at 1229.079 kb on - strand, within hlyE at 1229.256 kb on + strand, within hlyE
Per-strain Table
Position Strand Gene LocusTag Fraction 6 hr rep2_HD100_10fold_dilution_grewON remove 1,227,068 + ycgL b1179 0.45 +0.4 1,227,091 - ycgL b1179 0.52 -0.6 1,227,105 + ycgL b1179 0.57 -1.1 1,227,145 - ycgL b1179 0.71 -3.9 1,227,230 + +0.7 1,227,295 - -0.4 1,227,419 - ycgM b1180 0.18 -4.6 1,227,550 + ycgM b1180 0.38 +1.3 1,227,550 + ycgM b1180 0.38 +0.5 1,227,593 - ycgM b1180 0.44 +0.0 1,227,778 + ycgM b1180 0.72 +1.6 1,227,885 + -2.5 1,227,885 + ycgM b1180 0.88 -1.4 1,227,937 + +2.0 1,228,047 - -2.8 1,228,060 - +0.9 1,228,139 + ycgN b1181 0.22 +0.1 1,228,146 + ycgN b1181 0.23 +1.0 1,228,161 + ycgN b1181 0.27 -3.6 1,228,255 + ycgN b1181 0.47 +0.7 1,228,255 + ycgN b1181 0.47 -5.2 1,228,264 - ycgN b1181 0.49 -4.3 1,228,270 + ycgN b1181 0.50 -5.1 1,228,376 + ycgN b1181 0.73 +1.0 1,228,376 + ycgN b1181 0.73 +0.7 1,228,376 + ycgN b1181 0.73 +0.3 1,228,398 + ycgN b1181 0.78 -4.6 1,228,409 + ycgN b1181 0.80 -0.5 1,228,409 + ycgN b1181 0.80 -5.3 1,228,409 + ycgN b1181 0.80 -3.7 1,228,422 + ycgN b1181 0.83 +0.4 1,228,422 + ycgN b1181 0.83 -2.0 1,228,424 + ycgN b1181 0.84 -2.6 1,228,424 + ycgN b1181 0.84 -5.2 1,228,429 - ycgN b1181 0.85 +1.0 1,228,449 + ycgN b1181 0.89 +1.2 1,228,480 + +0.5 1,228,550 + +0.6 1,228,567 + +1.3 1,228,584 + -1.0 1,228,584 + -2.6 1,228,728 - -1.0 1,228,729 - -0.0 1,228,769 - +1.0 1,228,812 + hlyE b1182 0.12 +1.0 1,229,017 - hlyE b1182 0.34 +1.0 1,229,017 - hlyE b1182 0.34 +1.0 1,229,079 - hlyE b1182 0.41 -0.6 1,229,256 + hlyE b1182 0.60 +1.0
Or see this region's nucleotide sequence