Experiment: 6 hr rep2_HD100_10fold_dilution_grewON
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rihA and gltL are separated by 117 nucleotides gltL and gltK overlap by 1 nucleotides gltK and gltJ overlap by 1 nucleotides
b0651: rihA - ribonucleoside hydrolase 1 (NCBI), at 682,700 to 683,635
rihA
b0652: gltL - glutamate and aspartate transporter subunit (NCBI), at 683,753 to 684,478
gltL
b0653: gltK - glutamate and aspartate transporter subunit (NCBI), at 684,478 to 685,152
gltK
b0654: gltJ - glutamate and aspartate transporter subunit (NCBI), at 685,152 to 685,892
gltJ
Position (kb) 683
684
685 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2 at 682.763 kb on - strand at 682.763 kb on - strand at 682.763 kb on - strand at 682.773 kb on - strand at 682.781 kb on - strand at 682.833 kb on - strand, within rihA at 682.833 kb on - strand, within rihA at 682.833 kb on - strand, within rihA at 682.833 kb on - strand, within rihA at 682.850 kb on - strand, within rihA at 682.850 kb on - strand, within rihA at 682.859 kb on + strand, within rihA at 682.867 kb on - strand, within rihA at 682.869 kb on - strand, within rihA at 682.877 kb on - strand, within rihA at 682.882 kb on - strand, within rihA at 682.882 kb on - strand, within rihA at 682.892 kb on - strand, within rihA at 682.899 kb on + strand, within rihA at 682.901 kb on - strand, within rihA at 682.905 kb on + strand, within rihA at 682.906 kb on - strand, within rihA at 682.910 kb on - strand, within rihA at 682.910 kb on - strand, within rihA at 682.911 kb on + strand, within rihA at 682.911 kb on + strand, within rihA at 682.913 kb on - strand, within rihA at 682.913 kb on - strand, within rihA at 682.913 kb on - strand, within rihA at 682.913 kb on - strand, within rihA at 682.913 kb on - strand, within rihA at 682.913 kb on - strand, within rihA at 682.921 kb on - strand, within rihA at 682.921 kb on - strand, within rihA at 682.921 kb on - strand, within rihA at 682.921 kb on - strand, within rihA at 683.012 kb on - strand, within rihA at 683.065 kb on + strand, within rihA at 683.083 kb on - strand, within rihA at 683.083 kb on - strand, within rihA at 683.171 kb on + strand, within rihA at 683.178 kb on - strand at 683.269 kb on + strand, within rihA at 683.284 kb on - strand, within rihA at 683.570 kb on + strand at 683.575 kb on + strand at 683.595 kb on + strand at 683.629 kb on + strand at 683.741 kb on - strand at 683.759 kb on - strand at 683.810 kb on - strand at 684.018 kb on + strand, within gltL at 684.018 kb on + strand, within gltL at 684.026 kb on - strand, within gltL at 684.026 kb on - strand, within gltL at 684.222 kb on + strand, within gltL at 684.225 kb on - strand, within gltL at 684.225 kb on - strand, within gltL at 684.414 kb on + strand at 684.414 kb on + strand at 684.519 kb on + strand at 684.571 kb on - strand, within gltK at 684.631 kb on + strand, within gltK at 684.633 kb on - strand, within gltK at 684.650 kb on + strand, within gltK at 684.650 kb on + strand, within gltK at 684.658 kb on - strand, within gltK at 684.658 kb on - strand, within gltK at 684.660 kb on + strand, within gltK at 684.679 kb on - strand, within gltK at 684.679 kb on - strand, within gltK at 684.731 kb on - strand, within gltK at 684.818 kb on - strand, within gltK at 685.049 kb on + strand, within gltK at 685.049 kb on + strand, within gltK at 685.128 kb on - strand at 685.273 kb on - strand, within gltJ at 685.314 kb on + strand, within gltJ at 685.314 kb on + strand, within gltJ at 685.367 kb on + strand, within gltJ at 685.421 kb on + strand, within gltJ
Per-strain Table
Position Strand Gene LocusTag Fraction 6 hr rep2_HD100_10fold_dilution_grewON remove 682,763 - +0.1 682,763 - -0.2 682,763 - -4.3 682,773 - +0.5 682,781 - -0.3 682,833 - rihA b0651 0.14 -0.4 682,833 - rihA b0651 0.14 -3.5 682,833 - rihA b0651 0.14 +0.4 682,833 - rihA b0651 0.14 +1.1 682,850 - rihA b0651 0.16 -2.4 682,850 - rihA b0651 0.16 -0.2 682,859 + rihA b0651 0.17 -4.9 682,867 - rihA b0651 0.18 -2.6 682,869 - rihA b0651 0.18 +0.2 682,877 - rihA b0651 0.19 -3.0 682,882 - rihA b0651 0.19 +0.7 682,882 - rihA b0651 0.19 +0.6 682,892 - rihA b0651 0.21 +0.8 682,899 + rihA b0651 0.21 -1.1 682,901 - rihA b0651 0.21 -3.5 682,905 + rihA b0651 0.22 +1.2 682,906 - rihA b0651 0.22 -4.9 682,910 - rihA b0651 0.22 +2.1 682,910 - rihA b0651 0.22 -4.8 682,911 + rihA b0651 0.23 -1.4 682,911 + rihA b0651 0.23 -2.3 682,913 - rihA b0651 0.23 +1.2 682,913 - rihA b0651 0.23 +0.2 682,913 - rihA b0651 0.23 +0.3 682,913 - rihA b0651 0.23 +0.2 682,913 - rihA b0651 0.23 +1.2 682,913 - rihA b0651 0.23 +0.5 682,921 - rihA b0651 0.24 +0.4 682,921 - rihA b0651 0.24 -3.8 682,921 - rihA b0651 0.24 -3.7 682,921 - rihA b0651 0.24 +0.9 683,012 - rihA b0651 0.33 +0.8 683,065 + rihA b0651 0.39 -4.7 683,083 - rihA b0651 0.41 +0.5 683,083 - rihA b0651 0.41 +1.2 683,171 + rihA b0651 0.50 -6.2 683,178 - -1.4 683,269 + rihA b0651 0.61 +0.5 683,284 - rihA b0651 0.62 +1.9 683,570 + +0.3 683,575 + -0.1 683,595 + -0.6 683,629 + -0.3 683,741 - +1.2 683,759 - +0.3 683,810 - +1.1 684,018 + gltL b0652 0.37 -0.0 684,018 + gltL b0652 0.37 -3.4 684,026 - gltL b0652 0.38 -4.5 684,026 - gltL b0652 0.38 -5.3 684,222 + gltL b0652 0.65 +1.2 684,225 - gltL b0652 0.65 -1.9 684,225 - gltL b0652 0.65 -1.3 684,414 + +0.5 684,414 + +0.7 684,519 + +1.3 684,571 - gltK b0653 0.14 -0.1 684,631 + gltK b0653 0.23 +1.7 684,633 - gltK b0653 0.23 +0.2 684,650 + gltK b0653 0.25 +1.7 684,650 + gltK b0653 0.25 +0.8 684,658 - gltK b0653 0.27 -4.9 684,658 - gltK b0653 0.27 +1.4 684,660 + gltK b0653 0.27 +1.1 684,679 - gltK b0653 0.30 -4.1 684,679 - gltK b0653 0.30 +0.3 684,731 - gltK b0653 0.37 +1.0 684,818 - gltK b0653 0.50 +0.3 685,049 + gltK b0653 0.85 -1.8 685,049 + gltK b0653 0.85 +1.5 685,128 - -4.3 685,273 - gltJ b0654 0.16 -0.3 685,314 + gltJ b0654 0.22 -4.3 685,314 + gltJ b0654 0.22 -1.2 685,367 + gltJ b0654 0.29 +1.6 685,421 + gltJ b0654 0.36 +0.1
Or see this region's nucleotide sequence