Experiment: 6 hr rep2_HD100_10fold_dilution_grewON
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt caiC and caiB are separated by 73 nucleotides caiB and caiA are separated by 128 nucleotides
b0037: caiC - predicted crotonobetaine CoA ligase:carnitine CoA ligase (RefSeq), at 36,271 to 37,824
caiC
b0038: caiB - crotonobetainyl-CoA:carnitineCoA-transferase (NCBI), at 37,898 to 39,115
caiB
b0039: caiA - crotonobetaine reductase subunit II, FAD-binding (NCBI), at 39,244 to 40,386
caiA
Position (kb) 37
38
39
40 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2 at 36.924 kb on + strand, within caiC at 37.130 kb on + strand, within caiC at 37.155 kb on + strand, within caiC at 37.196 kb on + strand, within caiC at 37.219 kb on - strand, within caiC at 37.338 kb on - strand, within caiC at 37.460 kb on - strand, within caiC at 37.460 kb on - strand, within caiC at 37.463 kb on + strand, within caiC at 37.463 kb on + strand, within caiC at 37.480 kb on + strand, within caiC at 37.497 kb on + strand, within caiC at 37.534 kb on - strand, within caiC at 37.612 kb on + strand, within caiC at 37.623 kb on + strand, within caiC at 37.623 kb on + strand, within caiC at 37.634 kb on - strand, within caiC at 37.634 kb on - strand, within caiC at 37.985 kb on + strand at 37.993 kb on - strand at 37.993 kb on - strand at 38.049 kb on + strand, within caiB at 38.059 kb on - strand, within caiB at 38.098 kb on - strand, within caiB at 38.109 kb on + strand, within caiB at 38.143 kb on - strand, within caiB at 38.144 kb on + strand, within caiB at 38.144 kb on + strand, within caiB at 38.197 kb on - strand, within caiB at 38.197 kb on - strand, within caiB at 38.242 kb on + strand, within caiB at 38.279 kb on - strand, within caiB at 38.279 kb on - strand, within caiB at 38.286 kb on - strand, within caiB at 38.314 kb on + strand, within caiB at 38.325 kb on - strand, within caiB at 38.340 kb on - strand, within caiB at 38.361 kb on - strand, within caiB at 38.361 kb on - strand, within caiB at 38.368 kb on + strand, within caiB at 38.368 kb on - strand, within caiB at 38.372 kb on + strand, within caiB at 38.394 kb on + strand, within caiB at 38.428 kb on - strand, within caiB at 38.482 kb on - strand, within caiB at 38.494 kb on - strand, within caiB at 38.516 kb on - strand, within caiB at 38.564 kb on - strand, within caiB at 38.599 kb on - strand, within caiB at 38.699 kb on - strand, within caiB at 38.724 kb on + strand, within caiB at 38.732 kb on - strand, within caiB at 38.732 kb on - strand, within caiB at 38.771 kb on + strand, within caiB at 38.771 kb on + strand, within caiB at 38.829 kb on - strand, within caiB at 38.829 kb on - strand, within caiB at 38.831 kb on + strand, within caiB at 38.881 kb on - strand, within caiB at 38.941 kb on - strand, within caiB at 39.052 kb on - strand at 39.052 kb on - strand at 39.072 kb on + strand at 39.101 kb on + strand at 39.101 kb on + strand at 39.177 kb on - strand at 39.194 kb on + strand at 39.195 kb on + strand at 39.195 kb on + strand at 39.264 kb on - strand at 39.272 kb on - strand at 39.272 kb on - strand at 39.279 kb on - strand at 39.439 kb on - strand, within caiA at 39.472 kb on - strand, within caiA at 39.496 kb on - strand, within caiA at 39.589 kb on + strand, within caiA at 39.640 kb on - strand, within caiA at 39.640 kb on - strand, within caiA at 39.746 kb on - strand, within caiA at 39.800 kb on - strand, within caiA at 39.800 kb on - strand, within caiA at 39.849 kb on + strand, within caiA at 39.910 kb on - strand, within caiA at 39.982 kb on - strand, within caiA at 39.982 kb on - strand, within caiA
Per-strain Table
Position Strand Gene LocusTag Fraction 6 hr rep2_HD100_10fold_dilution_grewON remove 36,924 + caiC b0037 0.42 +2.2 37,130 + caiC b0037 0.55 -6.0 37,155 + caiC b0037 0.57 +0.8 37,196 + caiC b0037 0.60 -5.1 37,219 - caiC b0037 0.61 -5.7 37,338 - caiC b0037 0.69 +1.0 37,460 - caiC b0037 0.77 -3.2 37,460 - caiC b0037 0.77 +0.7 37,463 + caiC b0037 0.77 +1.3 37,463 + caiC b0037 0.77 +1.4 37,480 + caiC b0037 0.78 -0.3 37,497 + caiC b0037 0.79 -0.1 37,534 - caiC b0037 0.81 +0.3 37,612 + caiC b0037 0.86 +0.4 37,623 + caiC b0037 0.87 -3.0 37,623 + caiC b0037 0.87 -1.6 37,634 - caiC b0037 0.88 +0.8 37,634 - caiC b0037 0.88 +1.9 37,985 + +0.1 37,993 - +0.2 37,993 - -2.1 38,049 + caiB b0038 0.12 +0.5 38,059 - caiB b0038 0.13 +0.2 38,098 - caiB b0038 0.16 +0.6 38,109 + caiB b0038 0.17 -0.5 38,143 - caiB b0038 0.20 -6.2 38,144 + caiB b0038 0.20 +0.0 38,144 + caiB b0038 0.20 -0.1 38,197 - caiB b0038 0.25 -0.8 38,197 - caiB b0038 0.25 +0.0 38,242 + caiB b0038 0.28 +0.1 38,279 - caiB b0038 0.31 -0.6 38,279 - caiB b0038 0.31 +0.6 38,286 - caiB b0038 0.32 -0.0 38,314 + caiB b0038 0.34 -2.4 38,325 - caiB b0038 0.35 +1.3 38,340 - caiB b0038 0.36 +0.1 38,361 - caiB b0038 0.38 +1.0 38,361 - caiB b0038 0.38 +1.5 38,368 + caiB b0038 0.39 -1.3 38,368 - caiB b0038 0.39 +0.9 38,372 + caiB b0038 0.39 +0.4 38,394 + caiB b0038 0.41 +1.4 38,428 - caiB b0038 0.44 -0.9 38,482 - caiB b0038 0.48 -0.9 38,494 - caiB b0038 0.49 -0.3 38,516 - caiB b0038 0.51 -4.6 38,564 - caiB b0038 0.55 -0.9 38,599 - caiB b0038 0.58 +1.1 38,699 - caiB b0038 0.66 +0.4 38,724 + caiB b0038 0.68 -5.0 38,732 - caiB b0038 0.68 +1.3 38,732 - caiB b0038 0.68 -4.1 38,771 + caiB b0038 0.72 -4.8 38,771 + caiB b0038 0.72 +0.8 38,829 - caiB b0038 0.76 -2.2 38,829 - caiB b0038 0.76 +0.6 38,831 + caiB b0038 0.77 +1.2 38,881 - caiB b0038 0.81 +0.3 38,941 - caiB b0038 0.86 -0.2 39,052 - -4.1 39,052 - -2.4 39,072 + -0.5 39,101 + -4.9 39,101 + +0.9 39,177 - +1.8 39,194 + +1.0 39,195 + +1.2 39,195 + +1.1 39,264 - -1.6 39,272 - -2.8 39,272 - -2.4 39,279 - +1.7 39,439 - caiA b0039 0.17 -4.9 39,472 - caiA b0039 0.20 +0.3 39,496 - caiA b0039 0.22 -0.2 39,589 + caiA b0039 0.30 +1.7 39,640 - caiA b0039 0.35 +0.6 39,640 - caiA b0039 0.35 -3.8 39,746 - caiA b0039 0.44 -0.1 39,800 - caiA b0039 0.49 -4.4 39,800 - caiA b0039 0.49 -3.0 39,849 + caiA b0039 0.53 -0.5 39,910 - caiA b0039 0.58 -0.9 39,982 - caiA b0039 0.65 +1.2 39,982 - caiA b0039 0.65 -2.5
Or see this region's nucleotide sequence