Experiment: 6 hr rep1_HD100_10fold_dilution_grewON
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt bcsE and yhjT overlap by 4 nucleotides yhjT and bcsG overlap by 4 nucleotides
b3536: bcsE - hypothetical protein (NCBI), at 3,694,481 to 3,696,052
bcsE
b3537: yhjT - orf, hypothetical protein (VIMSS), at 3,696,049 to 3,696,240
yhjT
b3538: bcsG - predicted inner membrane protein (NCBI), at 3,696,237 to 3,697,916
bcsG
Position (kb)
3696
3697 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2
3 at 3695.072 kb on + strand, within bcsE at 3695.072 kb on - strand, within bcsE at 3695.072 kb on - strand, within bcsE at 3695.078 kb on - strand, within bcsE at 3695.085 kb on - strand, within bcsE at 3695.085 kb on - strand, within bcsE at 3695.085 kb on - strand, within bcsE at 3695.114 kb on - strand, within bcsE at 3695.175 kb on + strand, within bcsE at 3695.175 kb on + strand, within bcsE at 3695.177 kb on + strand, within bcsE at 3695.180 kb on + strand, within bcsE at 3695.182 kb on + strand, within bcsE at 3695.186 kb on - strand, within bcsE at 3695.186 kb on - strand, within bcsE at 3695.227 kb on + strand, within bcsE at 3695.251 kb on + strand, within bcsE at 3695.251 kb on + strand, within bcsE at 3695.251 kb on + strand, within bcsE at 3695.305 kb on + strand, within bcsE at 3695.336 kb on - strand, within bcsE at 3695.336 kb on - strand, within bcsE at 3695.336 kb on - strand, within bcsE at 3695.336 kb on - strand, within bcsE at 3695.336 kb on - strand, within bcsE at 3695.336 kb on - strand, within bcsE at 3695.352 kb on + strand, within bcsE at 3695.385 kb on - strand, within bcsE at 3695.460 kb on - strand, within bcsE at 3695.462 kb on - strand, within bcsE at 3695.462 kb on - strand, within bcsE at 3695.472 kb on + strand, within bcsE at 3695.480 kb on - strand, within bcsE at 3695.480 kb on - strand, within bcsE at 3695.520 kb on + strand, within bcsE at 3695.567 kb on - strand, within bcsE at 3695.567 kb on - strand, within bcsE at 3695.581 kb on + strand, within bcsE at 3695.582 kb on - strand, within bcsE at 3695.583 kb on + strand, within bcsE at 3695.591 kb on + strand, within bcsE at 3695.596 kb on + strand, within bcsE at 3695.632 kb on + strand, within bcsE at 3695.637 kb on - strand, within bcsE at 3695.637 kb on - strand, within bcsE at 3695.638 kb on - strand, within bcsE at 3695.644 kb on + strand, within bcsE at 3695.644 kb on + strand, within bcsE at 3695.700 kb on + strand, within bcsE at 3695.757 kb on + strand, within bcsE at 3695.814 kb on + strand, within bcsE at 3695.871 kb on - strand, within bcsE at 3695.894 kb on + strand, within bcsE at 3695.902 kb on - strand at 3695.902 kb on - strand at 3695.902 kb on - strand at 3695.926 kb on + strand at 3695.928 kb on - strand at 3695.938 kb on + strand at 3695.952 kb on + strand at 3695.952 kb on + strand at 3695.965 kb on + strand at 3695.985 kb on + strand at 3695.994 kb on + strand at 3696.022 kb on + strand at 3696.022 kb on + strand at 3696.032 kb on + strand at 3696.124 kb on - strand, within yhjT at 3696.124 kb on - strand, within yhjT at 3696.131 kb on + strand, within yhjT at 3696.131 kb on + strand, within yhjT at 3696.199 kb on + strand, within yhjT at 3696.205 kb on - strand, within yhjT at 3696.268 kb on + strand at 3696.292 kb on - strand at 3696.338 kb on + strand at 3696.338 kb on + strand at 3696.346 kb on - strand at 3696.346 kb on - strand at 3696.352 kb on + strand at 3696.416 kb on + strand, within bcsG at 3696.416 kb on + strand, within bcsG at 3696.416 kb on + strand, within bcsG at 3696.431 kb on - strand, within bcsG at 3696.445 kb on - strand, within bcsG at 3696.450 kb on - strand, within bcsG at 3696.473 kb on - strand, within bcsG at 3696.473 kb on - strand, within bcsG at 3696.473 kb on - strand, within bcsG at 3696.504 kb on - strand, within bcsG at 3696.519 kb on + strand, within bcsG at 3696.544 kb on - strand, within bcsG at 3696.581 kb on - strand, within bcsG at 3696.630 kb on - strand, within bcsG at 3696.677 kb on - strand, within bcsG at 3696.677 kb on - strand, within bcsG at 3696.681 kb on - strand, within bcsG at 3696.714 kb on + strand, within bcsG at 3696.753 kb on - strand, within bcsG at 3696.753 kb on - strand, within bcsG at 3696.771 kb on - strand, within bcsG at 3696.771 kb on - strand, within bcsG at 3696.804 kb on + strand, within bcsG at 3696.804 kb on + strand, within bcsG at 3696.829 kb on - strand, within bcsG at 3696.833 kb on + strand, within bcsG at 3696.833 kb on + strand, within bcsG at 3696.851 kb on + strand, within bcsG at 3696.925 kb on + strand, within bcsG at 3696.950 kb on + strand, within bcsG at 3696.980 kb on + strand at 3696.989 kb on - strand, within bcsG at 3697.005 kb on - strand, within bcsG at 3697.005 kb on - strand, within bcsG at 3697.052 kb on + strand, within bcsG at 3697.061 kb on + strand, within bcsG at 3697.062 kb on + strand, within bcsG at 3697.067 kb on - strand, within bcsG at 3697.069 kb on - strand, within bcsG at 3697.072 kb on + strand, within bcsG at 3697.084 kb on + strand, within bcsG at 3697.116 kb on + strand, within bcsG at 3697.120 kb on + strand, within bcsG at 3697.128 kb on - strand, within bcsG at 3697.138 kb on - strand, within bcsG
Per-strain Table
Position Strand Gene LocusTag Fraction 6 hr rep1_HD100_10fold_dilution_grewON remove 3,695,072 + bcsE b3536 0.38 -2.3 3,695,072 - bcsE b3536 0.38 -2.7 3,695,072 - bcsE b3536 0.38 +2.7 3,695,078 - bcsE b3536 0.38 +1.9 3,695,085 - bcsE b3536 0.38 +1.4 3,695,085 - bcsE b3536 0.38 +2.6 3,695,085 - bcsE b3536 0.38 +0.7 3,695,114 - bcsE b3536 0.40 -3.0 3,695,175 + bcsE b3536 0.44 -2.5 3,695,175 + bcsE b3536 0.44 +3.4 3,695,177 + bcsE b3536 0.44 -0.3 3,695,180 + bcsE b3536 0.44 +0.7 3,695,182 + bcsE b3536 0.45 +0.6 3,695,186 - bcsE b3536 0.45 -3.9 3,695,186 - bcsE b3536 0.45 -3.4 3,695,227 + bcsE b3536 0.47 +1.5 3,695,251 + bcsE b3536 0.49 -3.9 3,695,251 + bcsE b3536 0.49 +0.1 3,695,251 + bcsE b3536 0.49 -2.9 3,695,305 + bcsE b3536 0.52 +1.2 3,695,336 - bcsE b3536 0.54 +0.7 3,695,336 - bcsE b3536 0.54 +0.8 3,695,336 - bcsE b3536 0.54 +0.9 3,695,336 - bcsE b3536 0.54 +0.7 3,695,336 - bcsE b3536 0.54 +2.0 3,695,336 - bcsE b3536 0.54 +1.9 3,695,352 + bcsE b3536 0.55 -6.2 3,695,385 - bcsE b3536 0.58 +0.1 3,695,460 - bcsE b3536 0.62 -5.1 3,695,462 - bcsE b3536 0.62 -4.6 3,695,462 - bcsE b3536 0.62 +1.5 3,695,472 + bcsE b3536 0.63 -3.7 3,695,480 - bcsE b3536 0.64 -4.8 3,695,480 - bcsE b3536 0.64 +0.9 3,695,520 + bcsE b3536 0.66 +2.8 3,695,567 - bcsE b3536 0.69 -0.6 3,695,567 - bcsE b3536 0.69 -0.3 3,695,581 + bcsE b3536 0.70 +0.9 3,695,582 - bcsE b3536 0.70 +1.7 3,695,583 + bcsE b3536 0.70 +1.3 3,695,591 + bcsE b3536 0.71 -1.2 3,695,596 + bcsE b3536 0.71 +1.1 3,695,632 + bcsE b3536 0.73 +0.7 3,695,637 - bcsE b3536 0.74 +2.3 3,695,637 - bcsE b3536 0.74 -3.2 3,695,638 - bcsE b3536 0.74 -2.7 3,695,644 + bcsE b3536 0.74 -3.7 3,695,644 + bcsE b3536 0.74 -4.3 3,695,700 + bcsE b3536 0.78 +0.7 3,695,757 + bcsE b3536 0.81 -4.4 3,695,814 + bcsE b3536 0.85 +2.2 3,695,871 - bcsE b3536 0.88 -4.4 3,695,894 + bcsE b3536 0.90 +0.5 3,695,902 - -4.2 3,695,902 - +0.9 3,695,902 - +1.4 3,695,926 + +0.5 3,695,928 - -0.2 3,695,938 + +0.1 3,695,952 + -1.4 3,695,952 + +0.8 3,695,965 + -1.4 3,695,985 + +0.3 3,695,994 + -3.1 3,696,022 + -3.5 3,696,022 + +1.5 3,696,032 + +0.3 3,696,124 - yhjT b3537 0.39 +2.2 3,696,124 - yhjT b3537 0.39 +0.6 3,696,131 + yhjT b3537 0.43 -1.7 3,696,131 + yhjT b3537 0.43 +0.7 3,696,199 + yhjT b3537 0.78 -2.5 3,696,205 - yhjT b3537 0.81 +1.6 3,696,268 + +1.2 3,696,292 - -0.4 3,696,338 + +1.0 3,696,338 + +0.3 3,696,346 - +1.9 3,696,346 - -0.8 3,696,352 + -1.8 3,696,416 + bcsG b3538 0.11 +1.6 3,696,416 + bcsG b3538 0.11 -2.5 3,696,416 + bcsG b3538 0.11 +2.6 3,696,431 - bcsG b3538 0.12 -0.2 3,696,445 - bcsG b3538 0.12 +0.4 3,696,450 - bcsG b3538 0.13 +0.9 3,696,473 - bcsG b3538 0.14 +1.2 3,696,473 - bcsG b3538 0.14 +1.2 3,696,473 - bcsG b3538 0.14 -1.7 3,696,504 - bcsG b3538 0.16 -4.1 3,696,519 + bcsG b3538 0.17 +2.2 3,696,544 - bcsG b3538 0.18 +1.1 3,696,581 - bcsG b3538 0.20 +1.5 3,696,630 - bcsG b3538 0.23 -3.7 3,696,677 - bcsG b3538 0.26 +0.3 3,696,677 - bcsG b3538 0.26 -3.8 3,696,681 - bcsG b3538 0.26 +0.8 3,696,714 + bcsG b3538 0.28 -3.4 3,696,753 - bcsG b3538 0.31 -4.2 3,696,753 - bcsG b3538 0.31 +0.5 3,696,771 - bcsG b3538 0.32 +0.5 3,696,771 - bcsG b3538 0.32 +1.6 3,696,804 + bcsG b3538 0.34 +1.0 3,696,804 + bcsG b3538 0.34 -3.6 3,696,829 - bcsG b3538 0.35 -0.2 3,696,833 + bcsG b3538 0.35 -0.4 3,696,833 + bcsG b3538 0.35 +0.9 3,696,851 + bcsG b3538 0.37 +1.0 3,696,925 + bcsG b3538 0.41 +1.1 3,696,950 + bcsG b3538 0.42 -0.3 3,696,980 + +0.2 3,696,989 - bcsG b3538 0.45 -1.0 3,697,005 - bcsG b3538 0.46 +0.7 3,697,005 - bcsG b3538 0.46 +0.5 3,697,052 + bcsG b3538 0.49 -0.4 3,697,061 + bcsG b3538 0.49 -0.8 3,697,062 + bcsG b3538 0.49 -3.4 3,697,067 - bcsG b3538 0.49 +1.0 3,697,069 - bcsG b3538 0.50 +1.6 3,697,072 + bcsG b3538 0.50 +1.8 3,697,084 + bcsG b3538 0.50 +3.5 3,697,116 + bcsG b3538 0.52 -0.0 3,697,120 + bcsG b3538 0.53 +1.4 3,697,128 - bcsG b3538 0.53 -0.4 3,697,138 - bcsG b3538 0.54 -4.2
Or see this region's nucleotide sequence