Experiment: 6 hr rep1_HD100_10fold_dilution_grewON
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt atoD and atoA overlap by 1 nucleotides atoA and atoE overlap by 4 nucleotides atoE and atoB are separated by 30 nucleotides
b2221: atoD - acetyl-CoA:acetoacetyl-CoA transferase, alpha subunit (NCBI), at 2,321,469 to 2,322,131
atoD
b2222: atoA - acetyl-CoA:acetoacetyl-CoA transferase, beta subunit (NCBI), at 2,322,131 to 2,322,781
atoA
b2223: atoE - short chain fatty acid transporter (NCBI), at 2,322,778 to 2,324,100
atoE
b2224: atoB - acetyl-CoA acetyltransferase (NCBI), at 2,324,131 to 2,325,315
atoB
Position (kb)
2322
2323
2324
2325 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 2321.818 kb on + strand, within atoD at 2321.967 kb on + strand, within atoD at 2322.085 kb on - strand at 2322.255 kb on - strand, within atoA at 2322.362 kb on - strand, within atoA at 2322.391 kb on + strand, within atoA at 2322.410 kb on - strand, within atoA at 2322.423 kb on - strand, within atoA at 2322.629 kb on - strand, within atoA at 2322.892 kb on + strand at 2322.927 kb on + strand, within atoE at 2322.978 kb on - strand, within atoE at 2322.978 kb on - strand, within atoE at 2322.999 kb on + strand, within atoE at 2323.113 kb on - strand, within atoE at 2323.139 kb on + strand, within atoE at 2323.194 kb on - strand, within atoE at 2323.260 kb on + strand, within atoE at 2323.321 kb on - strand, within atoE at 2323.321 kb on - strand, within atoE at 2323.403 kb on + strand, within atoE at 2323.417 kb on - strand, within atoE at 2323.417 kb on - strand, within atoE at 2323.426 kb on - strand, within atoE at 2323.475 kb on - strand, within atoE at 2323.601 kb on + strand, within atoE at 2323.601 kb on + strand, within atoE at 2323.608 kb on + strand, within atoE at 2323.873 kb on + strand, within atoE at 2324.037 kb on + strand at 2324.037 kb on + strand at 2324.037 kb on + strand at 2324.148 kb on + strand at 2324.307 kb on - strand, within atoB at 2324.469 kb on + strand, within atoB at 2324.539 kb on - strand, within atoB at 2324.547 kb on + strand, within atoB at 2324.547 kb on + strand, within atoB at 2324.549 kb on + strand, within atoB at 2324.585 kb on - strand, within atoB at 2324.735 kb on + strand, within atoB at 2324.886 kb on - strand, within atoB at 2325.002 kb on - strand, within atoB at 2325.006 kb on - strand, within atoB at 2325.077 kb on - strand, within atoB at 2325.094 kb on + strand, within atoB
Per-strain Table
Position Strand Gene LocusTag Fraction 6 hr rep1_HD100_10fold_dilution_grewON remove 2,321,818 + atoD b2221 0.53 +1.8 2,321,967 + atoD b2221 0.75 -4.0 2,322,085 - -4.5 2,322,255 - atoA b2222 0.19 +0.9 2,322,362 - atoA b2222 0.35 -0.6 2,322,391 + atoA b2222 0.40 -4.9 2,322,410 - atoA b2222 0.43 +0.3 2,322,423 - atoA b2222 0.45 +1.3 2,322,629 - atoA b2222 0.76 -1.4 2,322,892 + +2.0 2,322,927 + atoE b2223 0.11 +1.1 2,322,978 - atoE b2223 0.15 -1.7 2,322,978 - atoE b2223 0.15 -3.6 2,322,999 + atoE b2223 0.17 +1.6 2,323,113 - atoE b2223 0.25 +0.9 2,323,139 + atoE b2223 0.27 +0.3 2,323,194 - atoE b2223 0.31 -1.3 2,323,260 + atoE b2223 0.36 +0.7 2,323,321 - atoE b2223 0.41 +0.8 2,323,321 - atoE b2223 0.41 -0.4 2,323,403 + atoE b2223 0.47 +0.3 2,323,417 - atoE b2223 0.48 -0.2 2,323,417 - atoE b2223 0.48 -2.8 2,323,426 - atoE b2223 0.49 +0.0 2,323,475 - atoE b2223 0.53 -4.3 2,323,601 + atoE b2223 0.62 -3.4 2,323,601 + atoE b2223 0.62 -2.6 2,323,608 + atoE b2223 0.63 -0.2 2,323,873 + atoE b2223 0.83 -2.9 2,324,037 + +0.8 2,324,037 + +1.9 2,324,037 + +1.6 2,324,148 + +0.6 2,324,307 - atoB b2224 0.15 +0.8 2,324,469 + atoB b2224 0.29 +0.6 2,324,539 - atoB b2224 0.34 +0.8 2,324,547 + atoB b2224 0.35 -4.8 2,324,547 + atoB b2224 0.35 +0.2 2,324,549 + atoB b2224 0.35 -0.1 2,324,585 - atoB b2224 0.38 -0.0 2,324,735 + atoB b2224 0.51 +1.1 2,324,886 - atoB b2224 0.64 +1.5 2,325,002 - atoB b2224 0.74 +1.7 2,325,006 - atoB b2224 0.74 +1.1 2,325,077 - atoB b2224 0.80 -4.7 2,325,094 + atoB b2224 0.81 -0.3
Or see this region's nucleotide sequence