Strain Fitness in Escherichia coli BW25113 around b2223

Experiment: 6 hr rep1_HD100_10fold_dilution_grewON

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntatoD and atoA overlap by 1 nucleotidesatoA and atoE overlap by 4 nucleotidesatoE and atoB are separated by 30 nucleotides b2221: atoD - acetyl-CoA:acetoacetyl-CoA transferase, alpha subunit (NCBI), at 2,321,469 to 2,322,131 atoD b2222: atoA - acetyl-CoA:acetoacetyl-CoA transferase, beta subunit (NCBI), at 2,322,131 to 2,322,781 atoA b2223: atoE - short chain fatty acid transporter (NCBI), at 2,322,778 to 2,324,100 atoE b2224: atoB - acetyl-CoA acetyltransferase (NCBI), at 2,324,131 to 2,325,315 atoB Position (kb) 2322 2323 2324 2325Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 2321.818 kb on + strand, within atoDat 2321.967 kb on + strand, within atoDat 2322.085 kb on - strandat 2322.255 kb on - strand, within atoAat 2322.362 kb on - strand, within atoAat 2322.391 kb on + strand, within atoAat 2322.410 kb on - strand, within atoAat 2322.423 kb on - strand, within atoAat 2322.629 kb on - strand, within atoAat 2322.892 kb on + strandat 2322.927 kb on + strand, within atoEat 2322.978 kb on - strand, within atoEat 2322.978 kb on - strand, within atoEat 2322.999 kb on + strand, within atoEat 2323.113 kb on - strand, within atoEat 2323.139 kb on + strand, within atoEat 2323.194 kb on - strand, within atoEat 2323.260 kb on + strand, within atoEat 2323.321 kb on - strand, within atoEat 2323.321 kb on - strand, within atoEat 2323.403 kb on + strand, within atoEat 2323.417 kb on - strand, within atoEat 2323.417 kb on - strand, within atoEat 2323.426 kb on - strand, within atoEat 2323.475 kb on - strand, within atoEat 2323.601 kb on + strand, within atoEat 2323.601 kb on + strand, within atoEat 2323.608 kb on + strand, within atoEat 2323.873 kb on + strand, within atoEat 2324.037 kb on + strandat 2324.037 kb on + strandat 2324.037 kb on + strandat 2324.148 kb on + strandat 2324.307 kb on - strand, within atoBat 2324.469 kb on + strand, within atoBat 2324.539 kb on - strand, within atoBat 2324.547 kb on + strand, within atoBat 2324.547 kb on + strand, within atoBat 2324.549 kb on + strand, within atoBat 2324.585 kb on - strand, within atoBat 2324.735 kb on + strand, within atoBat 2324.886 kb on - strand, within atoBat 2325.002 kb on - strand, within atoBat 2325.006 kb on - strand, within atoBat 2325.077 kb on - strand, within atoBat 2325.094 kb on + strand, within atoB

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction 6 hr rep1_HD100_10fold_dilution_grewON
remove
2,321,818 + atoD b2221 0.53 +1.8
2,321,967 + atoD b2221 0.75 -4.0
2,322,085 - -4.5
2,322,255 - atoA b2222 0.19 +0.9
2,322,362 - atoA b2222 0.35 -0.6
2,322,391 + atoA b2222 0.40 -4.9
2,322,410 - atoA b2222 0.43 +0.3
2,322,423 - atoA b2222 0.45 +1.3
2,322,629 - atoA b2222 0.76 -1.4
2,322,892 + +2.0
2,322,927 + atoE b2223 0.11 +1.1
2,322,978 - atoE b2223 0.15 -1.7
2,322,978 - atoE b2223 0.15 -3.6
2,322,999 + atoE b2223 0.17 +1.6
2,323,113 - atoE b2223 0.25 +0.9
2,323,139 + atoE b2223 0.27 +0.3
2,323,194 - atoE b2223 0.31 -1.3
2,323,260 + atoE b2223 0.36 +0.7
2,323,321 - atoE b2223 0.41 +0.8
2,323,321 - atoE b2223 0.41 -0.4
2,323,403 + atoE b2223 0.47 +0.3
2,323,417 - atoE b2223 0.48 -0.2
2,323,417 - atoE b2223 0.48 -2.8
2,323,426 - atoE b2223 0.49 +0.0
2,323,475 - atoE b2223 0.53 -4.3
2,323,601 + atoE b2223 0.62 -3.4
2,323,601 + atoE b2223 0.62 -2.6
2,323,608 + atoE b2223 0.63 -0.2
2,323,873 + atoE b2223 0.83 -2.9
2,324,037 + +0.8
2,324,037 + +1.9
2,324,037 + +1.6
2,324,148 + +0.6
2,324,307 - atoB b2224 0.15 +0.8
2,324,469 + atoB b2224 0.29 +0.6
2,324,539 - atoB b2224 0.34 +0.8
2,324,547 + atoB b2224 0.35 -4.8
2,324,547 + atoB b2224 0.35 +0.2
2,324,549 + atoB b2224 0.35 -0.1
2,324,585 - atoB b2224 0.38 -0.0
2,324,735 + atoB b2224 0.51 +1.1
2,324,886 - atoB b2224 0.64 +1.5
2,325,002 - atoB b2224 0.74 +1.7
2,325,006 - atoB b2224 0.74 +1.1
2,325,077 - atoB b2224 0.80 -4.7
2,325,094 + atoB b2224 0.81 -0.3

Or see this region's nucleotide sequence