Experiment: 6 hr rep1_HD100_10fold_dilution_grewON
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt wcaD and wcaC overlap by 26 nucleotides wcaC and wcaB overlap by 4 nucleotides wcaB and wcaA are separated by 2 nucleotides
b2056: wcaD - predicted colanic acid polymerase (NCBI), at 2,127,685 to 2,128,902
wcaD
b2057: wcaC - predicted glycosyl transferase (NCBI), at 2,128,877 to 2,130,094
wcaC
b2058: wcaB - predicted acyl transferase (NCBI), at 2,130,091 to 2,130,579
wcaB
b2059: wcaA - predicted glycosyl transferase (NCBI), at 2,130,582 to 2,131,421
wcaA
Position (kb)
2128
2129
2130
2131 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2128.019 kb on - strand, within wcaD at 2128.137 kb on - strand, within wcaD at 2128.137 kb on - strand, within wcaD at 2128.146 kb on - strand, within wcaD at 2128.222 kb on - strand, within wcaD at 2128.251 kb on - strand, within wcaD at 2128.259 kb on - strand, within wcaD at 2128.259 kb on - strand, within wcaD at 2128.280 kb on - strand, within wcaD at 2128.280 kb on - strand, within wcaD at 2128.280 kb on - strand, within wcaD at 2128.299 kb on - strand, within wcaD at 2128.304 kb on - strand, within wcaD at 2128.309 kb on - strand, within wcaD at 2128.324 kb on - strand, within wcaD at 2128.324 kb on - strand, within wcaD at 2128.355 kb on - strand, within wcaD at 2128.439 kb on - strand, within wcaD at 2128.439 kb on - strand, within wcaD at 2128.475 kb on - strand, within wcaD at 2128.527 kb on + strand, within wcaD at 2128.527 kb on + strand, within wcaD at 2128.530 kb on - strand, within wcaD at 2128.530 kb on - strand, within wcaD at 2128.531 kb on - strand, within wcaD at 2128.531 kb on - strand, within wcaD at 2128.531 kb on - strand, within wcaD at 2128.531 kb on - strand, within wcaD at 2128.535 kb on - strand, within wcaD at 2128.535 kb on - strand, within wcaD at 2128.545 kb on + strand, within wcaD at 2128.604 kb on + strand, within wcaD at 2128.604 kb on + strand, within wcaD at 2128.604 kb on + strand, within wcaD at 2128.604 kb on + strand, within wcaD at 2128.612 kb on - strand, within wcaD at 2128.612 kb on - strand, within wcaD at 2128.612 kb on - strand, within wcaD at 2128.614 kb on + strand, within wcaD at 2128.617 kb on + strand, within wcaD at 2128.667 kb on - strand, within wcaD at 2128.668 kb on - strand, within wcaD at 2128.690 kb on - strand, within wcaD at 2128.696 kb on + strand, within wcaD at 2128.696 kb on + strand, within wcaD at 2128.696 kb on + strand, within wcaD at 2128.702 kb on - strand, within wcaD at 2128.704 kb on - strand, within wcaD at 2128.713 kb on - strand, within wcaD at 2128.722 kb on + strand, within wcaD at 2128.918 kb on - strand at 2128.918 kb on - strand at 2128.925 kb on + strand at 2128.957 kb on + strand at 2128.968 kb on + strand at 2129.087 kb on - strand, within wcaC at 2129.249 kb on - strand, within wcaC at 2129.249 kb on - strand, within wcaC at 2129.459 kb on + strand, within wcaC at 2129.459 kb on + strand, within wcaC at 2129.468 kb on + strand, within wcaC at 2129.473 kb on + strand, within wcaC at 2129.586 kb on + strand, within wcaC at 2129.690 kb on + strand, within wcaC at 2129.798 kb on + strand, within wcaC at 2129.803 kb on + strand, within wcaC at 2130.018 kb on - strand at 2130.025 kb on + strand at 2130.025 kb on + strand at 2130.046 kb on - strand at 2130.159 kb on + strand, within wcaB at 2130.625 kb on + strand at 2130.691 kb on - strand, within wcaA at 2130.691 kb on - strand, within wcaA at 2130.692 kb on - strand, within wcaA at 2130.813 kb on + strand, within wcaA at 2130.841 kb on + strand, within wcaA at 2130.889 kb on - strand, within wcaA
Per-strain Table
Position Strand Gene LocusTag Fraction 6 hr rep1_HD100_10fold_dilution_grewON remove 2,128,019 - wcaD b2056 0.27 +1.6 2,128,137 - wcaD b2056 0.37 -0.4 2,128,137 - wcaD b2056 0.37 +1.6 2,128,146 - wcaD b2056 0.38 +1.6 2,128,222 - wcaD b2056 0.44 -2.0 2,128,251 - wcaD b2056 0.46 -1.7 2,128,259 - wcaD b2056 0.47 +1.6 2,128,259 - wcaD b2056 0.47 -1.6 2,128,280 - wcaD b2056 0.49 -1.0 2,128,280 - wcaD b2056 0.49 +0.6 2,128,280 - wcaD b2056 0.49 +1.6 2,128,299 - wcaD b2056 0.50 -2.3 2,128,304 - wcaD b2056 0.51 -2.9 2,128,309 - wcaD b2056 0.51 +0.9 2,128,324 - wcaD b2056 0.52 -2.0 2,128,324 - wcaD b2056 0.52 -1.7 2,128,355 - wcaD b2056 0.55 +2.4 2,128,439 - wcaD b2056 0.62 +1.6 2,128,439 - wcaD b2056 0.62 +0.3 2,128,475 - wcaD b2056 0.65 -3.3 2,128,527 + wcaD b2056 0.69 +1.6 2,128,527 + wcaD b2056 0.69 +3.6 2,128,530 - wcaD b2056 0.69 -3.5 2,128,530 - wcaD b2056 0.69 -4.1 2,128,531 - wcaD b2056 0.69 -0.7 2,128,531 - wcaD b2056 0.69 -4.5 2,128,531 - wcaD b2056 0.69 -2.7 2,128,531 - wcaD b2056 0.69 +1.6 2,128,535 - wcaD b2056 0.70 +3.4 2,128,535 - wcaD b2056 0.70 -3.1 2,128,545 + wcaD b2056 0.71 +0.6 2,128,604 + wcaD b2056 0.75 +1.6 2,128,604 + wcaD b2056 0.75 +1.6 2,128,604 + wcaD b2056 0.75 +1.6 2,128,604 + wcaD b2056 0.75 +0.6 2,128,612 - wcaD b2056 0.76 -1.9 2,128,612 - wcaD b2056 0.76 -1.6 2,128,612 - wcaD b2056 0.76 +0.5 2,128,614 + wcaD b2056 0.76 -1.6 2,128,617 + wcaD b2056 0.77 -0.4 2,128,667 - wcaD b2056 0.81 -4.3 2,128,668 - wcaD b2056 0.81 -1.6 2,128,690 - wcaD b2056 0.83 -4.6 2,128,696 + wcaD b2056 0.83 -2.7 2,128,696 + wcaD b2056 0.83 +1.8 2,128,696 + wcaD b2056 0.83 -1.2 2,128,702 - wcaD b2056 0.83 -0.5 2,128,704 - wcaD b2056 0.84 -1.9 2,128,713 - wcaD b2056 0.84 +2.8 2,128,722 + wcaD b2056 0.85 -0.1 2,128,918 - -3.1 2,128,918 - -0.9 2,128,925 + -1.4 2,128,957 + -4.4 2,128,968 + +0.7 2,129,087 - wcaC b2057 0.17 +1.2 2,129,249 - wcaC b2057 0.31 +1.6 2,129,249 - wcaC b2057 0.31 -3.3 2,129,459 + wcaC b2057 0.48 +3.2 2,129,459 + wcaC b2057 0.48 -4.1 2,129,468 + wcaC b2057 0.49 +1.1 2,129,473 + wcaC b2057 0.49 -4.0 2,129,586 + wcaC b2057 0.58 +0.8 2,129,690 + wcaC b2057 0.67 +3.8 2,129,798 + wcaC b2057 0.76 -1.2 2,129,803 + wcaC b2057 0.76 +1.2 2,130,018 - -4.7 2,130,025 + +4.6 2,130,025 + -2.3 2,130,046 - +1.4 2,130,159 + wcaB b2058 0.14 -4.6 2,130,625 + +0.4 2,130,691 - wcaA b2059 0.13 +2.2 2,130,691 - wcaA b2059 0.13 +0.7 2,130,692 - wcaA b2059 0.13 -0.8 2,130,813 + wcaA b2059 0.28 -2.6 2,130,841 + wcaA b2059 0.31 +0.2 2,130,889 - wcaA b2059 0.37 -0.5
Or see this region's nucleotide sequence