Experiment: 6 hr rep1_HD100_10fold_dilution_grewON
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hyaE and hyaF overlap by 4 nucleotides hyaF and appC are separated by 133 nucleotides appC and appB are separated by 11 nucleotides
b0976: hyaE - protein involved in processing of HyaA and HyaB proteins (NCBI), at 1,035,577 to 1,035,975
hyaE
b0977: hyaF - protein involved in nickel incorporation into hydrogenase-1 proteins (NCBI), at 1,035,972 to 1,036,829
hyaF
b0978: appC - cytochrome bd-II oxidase, subunit I (NCBI), at 1,036,963 to 1,038,507
appC
b0979: appB - cytochrome bd-II oxidase, subunit II (NCBI), at 1,038,519 to 1,039,655
appB
Position (kb)
1036
1037
1038
1039 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1035.990 kb on + strand at 1036.062 kb on - strand, within hyaF at 1036.216 kb on + strand, within hyaF at 1036.216 kb on + strand, within hyaF at 1036.275 kb on + strand, within hyaF at 1036.294 kb on + strand, within hyaF at 1036.394 kb on - strand, within hyaF at 1036.463 kb on + strand at 1036.690 kb on - strand, within hyaF at 1036.690 kb on - strand, within hyaF at 1036.774 kb on - strand at 1036.791 kb on - strand at 1036.863 kb on - strand at 1036.863 kb on - strand at 1037.041 kb on - strand at 1037.175 kb on + strand, within appC at 1037.240 kb on - strand, within appC at 1037.240 kb on - strand, within appC at 1037.262 kb on + strand, within appC at 1037.266 kb on + strand, within appC at 1037.367 kb on - strand, within appC at 1037.378 kb on + strand, within appC at 1037.396 kb on - strand, within appC at 1037.766 kb on + strand, within appC at 1037.904 kb on - strand, within appC at 1037.904 kb on - strand, within appC at 1037.934 kb on - strand, within appC at 1037.988 kb on - strand, within appC at 1037.988 kb on - strand, within appC at 1038.009 kb on - strand, within appC at 1038.093 kb on - strand, within appC at 1038.110 kb on - strand, within appC at 1038.170 kb on - strand, within appC at 1038.224 kb on - strand, within appC at 1038.248 kb on - strand, within appC at 1038.293 kb on - strand, within appC at 1038.293 kb on - strand, within appC at 1038.327 kb on - strand, within appC at 1038.336 kb on + strand, within appC at 1038.377 kb on + strand at 1038.395 kb on - strand at 1038.805 kb on - strand, within appB at 1039.201 kb on + strand, within appB at 1039.201 kb on + strand, within appB at 1039.201 kb on + strand, within appB at 1039.209 kb on - strand, within appB at 1039.209 kb on - strand, within appB at 1039.263 kb on + strand, within appB at 1039.453 kb on - strand, within appB at 1039.453 kb on - strand, within appB
Per-strain Table
Position Strand Gene LocusTag Fraction 6 hr rep1_HD100_10fold_dilution_grewON remove 1,035,990 + -0.6 1,036,062 - hyaF b0977 0.10 -0.2 1,036,216 + hyaF b0977 0.28 -2.5 1,036,216 + hyaF b0977 0.28 +1.1 1,036,275 + hyaF b0977 0.35 -0.9 1,036,294 + hyaF b0977 0.38 +1.4 1,036,394 - hyaF b0977 0.49 -2.4 1,036,463 + -2.4 1,036,690 - hyaF b0977 0.84 -3.4 1,036,690 - hyaF b0977 0.84 +0.9 1,036,774 - -4.1 1,036,791 - +1.4 1,036,863 - -2.7 1,036,863 - +0.4 1,037,041 - +0.3 1,037,175 + appC b0978 0.14 +1.2 1,037,240 - appC b0978 0.18 -1.4 1,037,240 - appC b0978 0.18 -0.7 1,037,262 + appC b0978 0.19 +1.6 1,037,266 + appC b0978 0.20 -3.7 1,037,367 - appC b0978 0.26 +0.6 1,037,378 + appC b0978 0.27 -4.1 1,037,396 - appC b0978 0.28 +0.9 1,037,766 + appC b0978 0.52 +3.1 1,037,904 - appC b0978 0.61 +1.2 1,037,904 - appC b0978 0.61 +1.9 1,037,934 - appC b0978 0.63 -4.6 1,037,988 - appC b0978 0.66 -4.2 1,037,988 - appC b0978 0.66 -3.6 1,038,009 - appC b0978 0.68 +0.3 1,038,093 - appC b0978 0.73 -4.7 1,038,110 - appC b0978 0.74 +1.4 1,038,170 - appC b0978 0.78 +0.4 1,038,224 - appC b0978 0.82 +1.2 1,038,248 - appC b0978 0.83 -3.8 1,038,293 - appC b0978 0.86 -2.9 1,038,293 - appC b0978 0.86 +0.2 1,038,327 - appC b0978 0.88 -0.2 1,038,336 + appC b0978 0.89 +0.3 1,038,377 + +0.2 1,038,395 - +1.0 1,038,805 - appB b0979 0.25 -1.6 1,039,201 + appB b0979 0.60 +0.3 1,039,201 + appB b0979 0.60 +0.6 1,039,201 + appB b0979 0.60 -4.6 1,039,209 - appB b0979 0.61 +3.0 1,039,209 - appB b0979 0.61 -2.0 1,039,263 + appB b0979 0.65 -1.4 1,039,453 - appB b0979 0.82 +0.9 1,039,453 - appB b0979 0.82 +1.2
Or see this region's nucleotide sequence