Experiment: Fraxetin 1 mM; solid stress
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_11345 and PS417_11350 are separated by 24 nucleotides PS417_11350 and PS417_11355 are separated by 11 nucleotides PS417_11355 and PS417_11360 are separated by 3 nucleotides
PS417_11345: PS417_11345 - membrane protein, at 2,443,306 to 2,444,889
_11345
PS417_11350: PS417_11350 - biopolymer transporter ExbB, at 2,444,914 to 2,446,713
_11350
PS417_11355: PS417_11355 - biopolymer transporter ExbD, at 2,446,725 to 2,447,150
_11355
PS417_11360: PS417_11360 - energy transducer TonB, at 2,447,154 to 2,447,858
_11360
Position (kb)
2444
2445
2446
2447 Strain fitness (log2 ratio)
-1
0
1
2 at 2443.934 kb on - strand, within PS417_11345 at 2444.450 kb on - strand at 2444.656 kb on + strand, within PS417_11345 at 2444.656 kb on + strand, within PS417_11345 at 2444.657 kb on - strand, within PS417_11345 at 2444.657 kb on - strand, within PS417_11345 at 2445.271 kb on + strand, within PS417_11350 at 2445.272 kb on - strand, within PS417_11350 at 2445.272 kb on - strand, within PS417_11350 at 2445.272 kb on - strand, within PS417_11350 at 2445.331 kb on + strand, within PS417_11350 at 2446.169 kb on - strand, within PS417_11350 at 2447.038 kb on + strand at 2447.039 kb on - strand, within PS417_11355 at 2447.596 kb on - strand, within PS417_11360 at 2447.598 kb on - strand, within PS417_11360 at 2447.619 kb on + strand, within PS417_11360
Per-strain Table
Position Strand Gene LocusTag Fraction Fraxetin 1 mM; solid stress remove 2,443,934 - PS417_11345 0.40 -0.5 2,444,450 - +0.7 2,444,656 + PS417_11345 0.85 -0.4 2,444,656 + PS417_11345 0.85 -0.4 2,444,657 - PS417_11345 0.85 -0.7 2,444,657 - PS417_11345 0.85 +0.0 2,445,271 + PS417_11350 0.20 +0.5 2,445,272 - PS417_11350 0.20 +1.9 2,445,272 - PS417_11350 0.20 +2.1 2,445,272 - PS417_11350 0.20 +0.4 2,445,331 + PS417_11350 0.23 +0.1 2,446,169 - PS417_11350 0.70 -0.2 2,447,038 + -0.2 2,447,039 - PS417_11355 0.74 +0.2 2,447,596 - PS417_11360 0.63 -0.5 2,447,598 - PS417_11360 0.63 -0.5 2,447,619 + PS417_11360 0.66 -0.3
Or see this region's nucleotide sequence