Experiment: 80% Methanol 1 mM; solid stress
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_14235 and PS417_14240 are separated by 254 nucleotides PS417_14240 and PS417_14245 are separated by 40 nucleotides PS417_14245 and PS417_14250 overlap by 1 nucleotides PS417_14250 and PS417_14255 are separated by 27 nucleotides
PS417_14235: PS417_14235 - hypothetical protein, at 3,116,992 to 3,117,189
_14235
PS417_14240: PS417_14240 - isochorismatase, at 3,117,444 to 3,118,049
_14240
PS417_14245: PS417_14245 - hypothetical protein, at 3,118,090 to 3,118,680
_14245
PS417_14250: PS417_14250 - hypothetical protein, at 3,118,680 to 3,119,378
_14250
PS417_14255: PS417_14255 - phosphate import ATP-binding protein PstB, at 3,119,406 to 3,120,167
_14255
Position (kb)
3118
3119 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3117.126 kb on + strand, within PS417_14235 at 3117.143 kb on - strand, within PS417_14235 at 3117.207 kb on - strand at 3117.207 kb on - strand at 3117.227 kb on + strand at 3117.228 kb on - strand at 3117.243 kb on + strand at 3117.243 kb on + strand at 3117.244 kb on - strand at 3117.244 kb on - strand at 3117.268 kb on - strand at 3117.341 kb on + strand at 3117.344 kb on - strand at 3117.353 kb on + strand at 3117.393 kb on + strand at 3117.393 kb on + strand at 3117.394 kb on - strand at 3117.394 kb on - strand at 3117.913 kb on - strand, within PS417_14240 at 3117.913 kb on - strand, within PS417_14240 at 3117.913 kb on - strand, within PS417_14240 at 3117.914 kb on + strand, within PS417_14240 at 3117.914 kb on + strand, within PS417_14240 at 3117.938 kb on + strand at 3118.047 kb on + strand at 3118.427 kb on + strand, within PS417_14245 at 3118.427 kb on + strand, within PS417_14245 at 3118.427 kb on + strand, within PS417_14245 at 3118.428 kb on - strand, within PS417_14245 at 3118.748 kb on + strand at 3118.748 kb on + strand at 3118.749 kb on - strand at 3118.795 kb on + strand, within PS417_14250 at 3118.912 kb on + strand, within PS417_14250 at 3118.980 kb on + strand, within PS417_14250 at 3118.981 kb on - strand, within PS417_14250 at 3118.981 kb on - strand, within PS417_14250 at 3118.985 kb on - strand, within PS417_14250 at 3118.985 kb on - strand, within PS417_14250 at 3118.985 kb on - strand, within PS417_14250 at 3119.032 kb on + strand, within PS417_14250 at 3119.041 kb on + strand, within PS417_14250 at 3119.042 kb on - strand, within PS417_14250 at 3119.110 kb on + strand, within PS417_14250 at 3119.146 kb on - strand, within PS417_14250 at 3119.215 kb on + strand, within PS417_14250 at 3119.215 kb on + strand, within PS417_14250 at 3119.216 kb on - strand, within PS417_14250 at 3119.216 kb on - strand, within PS417_14250 at 3119.216 kb on - strand, within PS417_14250 at 3119.429 kb on - strand at 3119.596 kb on + strand, within PS417_14255 at 3119.597 kb on - strand, within PS417_14255
Per-strain Table
Position Strand Gene LocusTag Fraction 80% Methanol 1 mM; solid stress remove 3,117,126 + PS417_14235 0.68 -0.2 3,117,143 - PS417_14235 0.76 +0.6 3,117,207 - -1.0 3,117,207 - +0.9 3,117,227 + +0.6 3,117,228 - +0.7 3,117,243 + -0.4 3,117,243 + -0.8 3,117,244 - +0.6 3,117,244 - -0.1 3,117,268 - -0.2 3,117,341 + -0.4 3,117,344 - +0.8 3,117,353 + -0.2 3,117,393 + -3.2 3,117,393 + +0.1 3,117,394 - -0.4 3,117,394 - -0.3 3,117,913 - PS417_14240 0.77 -0.2 3,117,913 - PS417_14240 0.77 +0.3 3,117,913 - PS417_14240 0.77 -0.5 3,117,914 + PS417_14240 0.78 +0.1 3,117,914 + PS417_14240 0.78 +0.9 3,117,938 + +0.2 3,118,047 + -0.5 3,118,427 + PS417_14245 0.57 -1.1 3,118,427 + PS417_14245 0.57 -0.4 3,118,427 + PS417_14245 0.57 +1.0 3,118,428 - PS417_14245 0.57 -0.8 3,118,748 + -0.2 3,118,748 + +0.2 3,118,749 - -0.6 3,118,795 + PS417_14250 0.16 +0.6 3,118,912 + PS417_14250 0.33 +0.7 3,118,980 + PS417_14250 0.43 +0.2 3,118,981 - PS417_14250 0.43 -0.0 3,118,981 - PS417_14250 0.43 +0.3 3,118,985 - PS417_14250 0.44 -0.0 3,118,985 - PS417_14250 0.44 -0.7 3,118,985 - PS417_14250 0.44 +0.1 3,119,032 + PS417_14250 0.50 -0.1 3,119,041 + PS417_14250 0.52 +0.7 3,119,042 - PS417_14250 0.52 -0.2 3,119,110 + PS417_14250 0.62 -0.7 3,119,146 - PS417_14250 0.67 -0.3 3,119,215 + PS417_14250 0.77 -0.9 3,119,215 + PS417_14250 0.77 +0.5 3,119,216 - PS417_14250 0.77 +0.3 3,119,216 - PS417_14250 0.77 +0.2 3,119,216 - PS417_14250 0.77 -0.3 3,119,429 - -0.2 3,119,596 + PS417_14255 0.25 -0.1 3,119,597 - PS417_14255 0.25 +0.6
Or see this region's nucleotide sequence