Experiment: 80% Methanol 3 mM; liquid stress
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_01025 and PS417_01030 overlap by 4 nucleotides PS417_01030 and PS417_01035 are separated by 99 nucleotides PS417_01035 and PS417_01040 are separated by 121 nucleotides
PS417_01025: PS417_01025 - glutamine ABC transporter ATP-binding protein, at 223,171 to 223,947
_01025
PS417_01030: PS417_01030 - glutamine ABC transporter substrate-binding protein, at 223,944 to 224,798
_01030
PS417_01035: PS417_01035 - ATP-binding protein, at 224,898 to 226,058
_01035
PS417_01040: PS417_01040 - RND transporter, at 226,180 to 227,262
_01040
Position (kb) 224
225
226
227 Strain fitness (log2 ratio) -2
-1
0
1 at 223.944 kb on - strand at 224.134 kb on - strand, within PS417_01030 at 224.433 kb on - strand, within PS417_01030 at 224.724 kb on + strand at 224.724 kb on + strand at 224.725 kb on - strand at 224.725 kb on - strand at 224.838 kb on + strand at 224.838 kb on + strand at 224.838 kb on + strand at 224.839 kb on - strand at 224.839 kb on - strand at 224.887 kb on - strand at 224.899 kb on + strand at 224.900 kb on - strand at 224.988 kb on - strand at 225.184 kb on + strand, within PS417_01035 at 225.553 kb on + strand, within PS417_01035 at 225.553 kb on + strand, within PS417_01035 at 225.554 kb on - strand, within PS417_01035 at 225.554 kb on - strand, within PS417_01035 at 226.110 kb on + strand at 226.125 kb on + strand at 226.126 kb on - strand at 226.339 kb on - strand, within PS417_01040 at 226.542 kb on - strand, within PS417_01040 at 226.570 kb on + strand, within PS417_01040 at 226.570 kb on + strand, within PS417_01040 at 226.570 kb on + strand, within PS417_01040 at 226.571 kb on - strand, within PS417_01040 at 226.746 kb on - strand, within PS417_01040 at 226.811 kb on - strand, within PS417_01040 at 226.811 kb on - strand, within PS417_01040 at 226.811 kb on - strand, within PS417_01040 at 226.929 kb on + strand, within PS417_01040
Per-strain Table
Position Strand Gene LocusTag Fraction 80% Methanol 3 mM; liquid stress remove 223,944 - -2.2 224,134 - PS417_01030 0.22 -0.3 224,433 - PS417_01030 0.57 -0.5 224,724 + -0.3 224,724 + -0.2 224,725 - -0.1 224,725 - -0.1 224,838 + +0.1 224,838 + -0.2 224,838 + -0.4 224,839 - +0.1 224,839 - +0.0 224,887 - +0.9 224,899 + +0.3 224,900 - -0.4 224,988 - -0.1 225,184 + PS417_01035 0.25 -0.9 225,553 + PS417_01035 0.56 +1.0 225,553 + PS417_01035 0.56 -0.1 225,554 - PS417_01035 0.57 -1.0 225,554 - PS417_01035 0.57 -0.3 226,110 + -1.3 226,125 + -0.7 226,126 - +0.0 226,339 - PS417_01040 0.15 -0.1 226,542 - PS417_01040 0.33 +0.0 226,570 + PS417_01040 0.36 -0.1 226,570 + PS417_01040 0.36 -0.7 226,570 + PS417_01040 0.36 -1.4 226,571 - PS417_01040 0.36 +0.2 226,746 - PS417_01040 0.52 -0.3 226,811 - PS417_01040 0.58 -2.0 226,811 - PS417_01040 0.58 -0.9 226,811 - PS417_01040 0.58 -0.6 226,929 + PS417_01040 0.69 -0.2
Or see this region's nucleotide sequence