Experiment: Fraxetin 3 mM; solid stress
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_27060 and PS417_27065 are separated by 131 nucleotides PS417_27065 and PS417_27070 are separated by 20 nucleotides PS417_27070 and PS417_27075 are separated by 99 nucleotides PS417_27075 and PS417_27080 are separated by 30 nucleotides
PS417_27060: PS417_27060 - 2OG-Fe(II) oxygenase, at 5,842,187 to 5,842,819
_27060
PS417_27065: PS417_27065 - hypothetical protein, at 5,842,951 to 5,843,703
_27065
PS417_27070: PS417_27070 - alpha/beta hydrolase, at 5,843,724 to 5,844,650
_27070
PS417_27075: PS417_27075 - thiol-disulfide isomerase, at 5,844,750 to 5,845,325
_27075
PS417_27080: PS417_27080 - penicillin amidase, at 5,845,356 to 5,847,710
_27080
Position (kb)
5843
5844
5845 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 5842.774 kb on + strand at 5842.774 kb on + strand at 5842.774 kb on + strand at 5842.774 kb on + strand at 5842.775 kb on - strand at 5842.775 kb on - strand at 5842.775 kb on - strand at 5842.802 kb on + strand at 5842.803 kb on - strand at 5842.819 kb on + strand at 5842.819 kb on + strand at 5842.820 kb on - strand at 5842.822 kb on + strand at 5842.823 kb on - strand at 5842.823 kb on - strand at 5842.823 kb on - strand at 5842.828 kb on + strand at 5842.866 kb on + strand at 5842.874 kb on - strand at 5842.874 kb on - strand at 5842.927 kb on - strand at 5843.023 kb on + strand at 5843.024 kb on - strand at 5843.024 kb on - strand at 5843.120 kb on - strand, within PS417_27065 at 5843.120 kb on - strand, within PS417_27065 at 5843.554 kb on + strand, within PS417_27065 at 5843.555 kb on - strand, within PS417_27065 at 5843.555 kb on - strand, within PS417_27065 at 5843.630 kb on - strand at 5843.632 kb on + strand at 5843.633 kb on - strand at 5843.633 kb on - strand at 5843.633 kb on - strand at 5843.718 kb on + strand at 5843.719 kb on - strand at 5843.725 kb on + strand at 5843.725 kb on + strand at 5843.725 kb on + strand at 5843.726 kb on - strand at 5843.726 kb on - strand at 5843.753 kb on + strand at 5843.753 kb on + strand at 5843.753 kb on + strand at 5843.753 kb on + strand at 5843.753 kb on + strand at 5843.754 kb on - strand at 5843.754 kb on - strand at 5843.754 kb on - strand at 5843.754 kb on - strand at 5843.754 kb on - strand at 5843.754 kb on - strand at 5843.759 kb on - strand at 5843.759 kb on - strand at 5844.091 kb on + strand, within PS417_27070 at 5844.091 kb on + strand, within PS417_27070 at 5844.092 kb on - strand, within PS417_27070 at 5844.094 kb on + strand, within PS417_27070 at 5844.094 kb on + strand, within PS417_27070 at 5844.094 kb on + strand, within PS417_27070 at 5844.095 kb on - strand, within PS417_27070 at 5844.095 kb on - strand, within PS417_27070 at 5844.095 kb on - strand, within PS417_27070 at 5844.095 kb on - strand, within PS417_27070 at 5844.095 kb on - strand, within PS417_27070 at 5844.298 kb on + strand, within PS417_27070 at 5844.431 kb on - strand, within PS417_27070 at 5844.650 kb on + strand at 5844.651 kb on - strand at 5844.724 kb on + strand at 5844.725 kb on - strand at 5844.725 kb on - strand at 5844.725 kb on - strand at 5844.741 kb on + strand at 5844.742 kb on - strand at 5844.742 kb on - strand at 5844.826 kb on - strand, within PS417_27075 at 5844.826 kb on - strand, within PS417_27075 at 5845.035 kb on + strand, within PS417_27075 at 5845.035 kb on + strand, within PS417_27075 at 5845.188 kb on + strand, within PS417_27075 at 5845.208 kb on + strand, within PS417_27075 at 5845.209 kb on - strand, within PS417_27075 at 5845.226 kb on + strand, within PS417_27075 at 5845.267 kb on + strand, within PS417_27075 at 5845.268 kb on - strand at 5845.419 kb on + strand at 5845.419 kb on + strand at 5845.419 kb on + strand at 5845.420 kb on - strand at 5845.425 kb on + strand at 5845.425 kb on + strand at 5845.560 kb on + strand at 5845.560 kb on + strand at 5845.560 kb on + strand at 5845.561 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fraxetin 3 mM; solid stress remove 5,842,774 + -0.2 5,842,774 + -0.2 5,842,774 + +0.7 5,842,774 + +0.7 5,842,775 - -0.1 5,842,775 - -0.2 5,842,775 - -0.4 5,842,802 + +1.0 5,842,803 - +1.6 5,842,819 + +0.1 5,842,819 + -1.1 5,842,820 - -0.4 5,842,822 + -0.1 5,842,823 - -0.3 5,842,823 - -0.0 5,842,823 - +1.0 5,842,828 + -2.2 5,842,866 + -0.6 5,842,874 - +0.1 5,842,874 - -0.8 5,842,927 - -0.2 5,843,023 + -0.6 5,843,024 - -0.1 5,843,024 - -2.4 5,843,120 - PS417_27065 0.22 -0.8 5,843,120 - PS417_27065 0.22 -1.2 5,843,554 + PS417_27065 0.80 -0.4 5,843,555 - PS417_27065 0.80 -1.1 5,843,555 - PS417_27065 0.80 -0.4 5,843,630 - -0.4 5,843,632 + +0.3 5,843,633 - -0.8 5,843,633 - -0.8 5,843,633 - -1.3 5,843,718 + +1.0 5,843,719 - -0.2 5,843,725 + -0.9 5,843,725 + -0.3 5,843,725 + +0.0 5,843,726 - -1.2 5,843,726 - +0.3 5,843,753 + -0.2 5,843,753 + -0.6 5,843,753 + -1.2 5,843,753 + +1.5 5,843,753 + -0.1 5,843,754 - -1.1 5,843,754 - -1.0 5,843,754 - -0.5 5,843,754 - -0.7 5,843,754 - -0.7 5,843,754 - -0.3 5,843,759 - -1.3 5,843,759 - +1.3 5,844,091 + PS417_27070 0.40 -1.1 5,844,091 + PS417_27070 0.40 -1.4 5,844,092 - PS417_27070 0.40 +0.1 5,844,094 + PS417_27070 0.40 -1.1 5,844,094 + PS417_27070 0.40 -1.2 5,844,094 + PS417_27070 0.40 -3.9 5,844,095 - PS417_27070 0.40 -0.8 5,844,095 - PS417_27070 0.40 -1.3 5,844,095 - PS417_27070 0.40 -0.8 5,844,095 - PS417_27070 0.40 -1.4 5,844,095 - PS417_27070 0.40 -2.0 5,844,298 + PS417_27070 0.62 -1.2 5,844,431 - PS417_27070 0.76 +0.0 5,844,650 + -0.0 5,844,651 - -0.2 5,844,724 + -0.4 5,844,725 - -1.0 5,844,725 - +0.8 5,844,725 - +1.1 5,844,741 + +1.0 5,844,742 - +1.7 5,844,742 - +1.2 5,844,826 - PS417_27075 0.13 +1.3 5,844,826 - PS417_27075 0.13 +1.0 5,845,035 + PS417_27075 0.49 +1.6 5,845,035 + PS417_27075 0.49 +1.5 5,845,188 + PS417_27075 0.76 +0.7 5,845,208 + PS417_27075 0.80 -0.0 5,845,209 - PS417_27075 0.80 +1.7 5,845,226 + PS417_27075 0.83 +0.6 5,845,267 + PS417_27075 0.90 +0.5 5,845,268 - +0.1 5,845,419 + -0.1 5,845,419 + +0.3 5,845,419 + -1.2 5,845,420 - -0.7 5,845,425 + -0.0 5,845,425 + -0.1 5,845,560 + -1.7 5,845,560 + -1.3 5,845,560 + +0.1 5,845,561 - -0.2
Or see this region's nucleotide sequence