Experiment: Fraxetin 3 mM; solid stress
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_22425 and PS417_22430 overlap by 1 nucleotides PS417_22430 and PS417_22435 are separated by 14 nucleotides PS417_22435 and PS417_22440 are separated by 1 nucleotides PS417_22440 and PS417_22445 are separated by 139 nucleotides
PS417_22425: PS417_22425 - cell envelope biogenesis protein TolA, at 4,857,889 to 4,858,962
_22425
PS417_22430: PS417_22430 - biopolymer transporter TolR, at 4,858,962 to 4,859,423
_22430
PS417_22435: PS417_22435 - protein tolQ, at 4,859,438 to 4,860,133
_22435
PS417_22440: PS417_22440 - 4-hydroxybenzoyl-CoA thioesterase, at 4,860,135 to 4,860,590
_22440
PS417_22445: PS417_22445 - ATP-dependent DNA helicase RuvB, at 4,860,730 to 4,861,788
_22445
Position (kb) 4859
4860
4861 Strain fitness (log2 ratio) -1
0
1
2 at 4859.440 kb on - strand at 4860.193 kb on - strand, within PS417_22440 at 4860.591 kb on - strand at 4860.591 kb on - strand at 4860.603 kb on - strand at 4860.703 kb on + strand at 4860.708 kb on + strand at 4860.709 kb on - strand at 4861.122 kb on + strand, within PS417_22445
Per-strain Table
Position Strand Gene LocusTag Fraction Fraxetin 3 mM; solid stress remove 4,859,440 - -0.5 4,860,193 - PS417_22440 0.13 -1.2 4,860,591 - +0.7 4,860,591 - +0.2 4,860,603 - -1.3 4,860,703 + +0.8 4,860,708 + +0.3 4,860,709 - +2.7 4,861,122 + PS417_22445 0.37 +0.5
Or see this region's nucleotide sequence