Strain Fitness in Pseudomonas simiae WCS417 around PS417_09200

Experiment: Fraxetin 3 mM; solid stress

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_09190 and PS417_09195 are separated by 57 nucleotidesPS417_09195 and PS417_09200 are separated by 134 nucleotidesPS417_09200 and PS417_09205 overlap by 4 nucleotidesPS417_09205 and PS417_09210 are separated by 135 nucleotides PS417_09190: PS417_09190 - cysteine protease, at 1,997,985 to 1,998,644 _09190 PS417_09195: PS417_09195 - glutathione S-transferase, at 1,998,702 to 1,999,319 _09195 PS417_09200: PS417_09200 - ABC transporter, at 1,999,454 to 2,000,386 _09200 PS417_09205: PS417_09205 - membrane protein, at 2,000,383 to 2,001,162 _09205 PS417_09210: PS417_09210 - TetR family transcriptional regulator, at 2,001,298 to 2,001,837 _09210 Position (kb) 1999 2000 2001Strain fitness (log2 ratio) -2 -1 0 1at 1998.670 kb on + strandat 1998.671 kb on - strandat 1998.671 kb on - strandat 1998.671 kb on - strandat 1998.781 kb on - strand, within PS417_09195at 1999.045 kb on + strand, within PS417_09195at 1999.045 kb on + strand, within PS417_09195at 1999.124 kb on - strand, within PS417_09195at 1999.139 kb on + strand, within PS417_09195at 1999.140 kb on - strand, within PS417_09195at 1999.204 kb on - strand, within PS417_09195at 1999.204 kb on - strand, within PS417_09195at 1999.250 kb on - strand, within PS417_09195at 1999.250 kb on - strand, within PS417_09195at 1999.261 kb on - strandat 1999.261 kb on - strandat 1999.317 kb on + strandat 1999.318 kb on - strandat 1999.318 kb on - strandat 1999.327 kb on + strandat 1999.345 kb on + strandat 1999.345 kb on + strandat 1999.346 kb on - strandat 1999.418 kb on - strandat 1999.426 kb on + strandat 1999.426 kb on + strandat 1999.427 kb on - strandat 1999.446 kb on - strandat 1999.642 kb on + strand, within PS417_09200at 1999.643 kb on - strand, within PS417_09200at 1999.811 kb on + strand, within PS417_09200at 1999.943 kb on - strand, within PS417_09200at 1999.943 kb on - strand, within PS417_09200at 2000.452 kb on - strandat 2000.563 kb on + strand, within PS417_09205at 2000.563 kb on + strand, within PS417_09205at 2000.572 kb on + strand, within PS417_09205at 2000.572 kb on + strand, within PS417_09205at 2000.572 kb on + strand, within PS417_09205at 2000.572 kb on + strand, within PS417_09205at 2000.573 kb on - strand, within PS417_09205at 2000.617 kb on + strand, within PS417_09205at 2000.700 kb on + strand, within PS417_09205at 2000.898 kb on + strand, within PS417_09205at 2000.957 kb on - strand, within PS417_09205at 2000.957 kb on - strand, within PS417_09205at 2001.112 kb on + strandat 2001.112 kb on + strandat 2001.112 kb on + strandat 2001.113 kb on - strandat 2001.113 kb on - strandat 2001.131 kb on + strandat 2001.235 kb on + strandat 2001.236 kb on - strandat 2001.328 kb on - strandat 2001.328 kb on - strandat 2001.365 kb on - strand, within PS417_09210at 2001.369 kb on + strand, within PS417_09210

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Per-strain Table

Position Strand Gene LocusTag Fraction Fraxetin 3 mM; solid stress
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1,998,670 + -0.4
1,998,671 - -0.5
1,998,671 - +0.8
1,998,671 - +0.9
1,998,781 - PS417_09195 0.13 +0.0
1,999,045 + PS417_09195 0.56 +1.3
1,999,045 + PS417_09195 0.56 +0.3
1,999,124 - PS417_09195 0.68 +0.5
1,999,139 + PS417_09195 0.71 -1.9
1,999,140 - PS417_09195 0.71 +0.5
1,999,204 - PS417_09195 0.81 +0.2
1,999,204 - PS417_09195 0.81 -0.2
1,999,250 - PS417_09195 0.89 -0.2
1,999,250 - PS417_09195 0.89 -0.5
1,999,261 - -0.1
1,999,261 - -0.3
1,999,317 + -0.3
1,999,318 - +1.3
1,999,318 - -1.2
1,999,327 + +0.1
1,999,345 + +1.7
1,999,345 + +0.8
1,999,346 - -1.2
1,999,418 - -0.3
1,999,426 + -0.3
1,999,426 + -0.3
1,999,427 - -0.9
1,999,446 - -0.8
1,999,642 + PS417_09200 0.20 -1.4
1,999,643 - PS417_09200 0.20 -1.1
1,999,811 + PS417_09200 0.38 -0.4
1,999,943 - PS417_09200 0.52 -1.4
1,999,943 - PS417_09200 0.52 -1.0
2,000,452 - +1.0
2,000,563 + PS417_09205 0.23 -0.7
2,000,563 + PS417_09205 0.23 -0.8
2,000,572 + PS417_09205 0.24 -1.4
2,000,572 + PS417_09205 0.24 -0.7
2,000,572 + PS417_09205 0.24 +0.2
2,000,572 + PS417_09205 0.24 -1.2
2,000,573 - PS417_09205 0.24 +0.1
2,000,617 + PS417_09205 0.30 -0.4
2,000,700 + PS417_09205 0.41 -0.2
2,000,898 + PS417_09205 0.66 +0.1
2,000,957 - PS417_09205 0.74 -0.3
2,000,957 - PS417_09205 0.74 -0.1
2,001,112 + -0.7
2,001,112 + +0.2
2,001,112 + -1.0
2,001,113 - -2.1
2,001,113 - -1.1
2,001,131 + +0.2
2,001,235 + -0.0
2,001,236 - -0.0
2,001,328 - +0.3
2,001,328 - -1.2
2,001,365 - PS417_09210 0.12 -0.8
2,001,369 + PS417_09210 0.13 -0.6

Or see this region's nucleotide sequence