Strain Fitness in Pseudomonas simiae WCS417 around PS417_27835

Experiment: 80% Methanol 3 mM; liquid stress

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_27830 and PS417_27835 are separated by 24 nucleotidesPS417_27835 and PS417_27840 are separated by 56 nucleotidesPS417_27840 and PS417_27845 are separated by 124 nucleotides PS417_27830: PS417_27830 - (p)ppGpp synthetase, at 5,999,083 to 6,001,188 _27830 PS417_27835: PS417_27835 - endoribonuclease, at 6,001,213 to 6,001,593 _27835 PS417_27840: PS417_27840 - hypothetical protein, at 6,001,650 to 6,002,393 _27840 PS417_27845: PS417_27845 - oxidoreductase, at 6,002,518 to 6,003,369 _27845 Position (kb) 6001 6002Strain fitness (log2 ratio) -2 -1 0 1at 6001.195 kb on + strandat 6001.198 kb on + strandat 6001.198 kb on + strandat 6001.199 kb on - strandat 6001.199 kb on - strandat 6001.228 kb on - strandat 6001.265 kb on - strand, within PS417_27835at 6001.265 kb on - strand, within PS417_27835at 6001.297 kb on + strand, within PS417_27835at 6001.298 kb on - strand, within PS417_27835at 6001.367 kb on + strand, within PS417_27835at 6001.496 kb on - strand, within PS417_27835at 6001.496 kb on - strandat 6001.496 kb on - strand, within PS417_27835at 6001.666 kb on + strandat 6001.667 kb on - strandat 6001.836 kb on - strand, within PS417_27840at 6001.858 kb on - strand, within PS417_27840at 6001.860 kb on + strand, within PS417_27840at 6001.861 kb on - strand, within PS417_27840at 6002.076 kb on + strand, within PS417_27840at 6002.077 kb on - strand, within PS417_27840at 6002.085 kb on + strand, within PS417_27840at 6002.086 kb on - strand, within PS417_27840at 6002.086 kb on - strand, within PS417_27840at 6002.137 kb on + strand, within PS417_27840at 6002.138 kb on - strand, within PS417_27840at 6002.138 kb on - strand, within PS417_27840at 6002.391 kb on + strandat 6002.391 kb on + strandat 6002.391 kb on + strandat 6002.392 kb on - strandat 6002.392 kb on - strandat 6002.589 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 80% Methanol 3 mM; liquid stress
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6,001,195 + +1.6
6,001,198 + -0.0
6,001,198 + -1.3
6,001,199 - +1.4
6,001,199 - +0.3
6,001,228 - -0.9
6,001,265 - PS417_27835 0.14 -2.6
6,001,265 - PS417_27835 0.14 -0.7
6,001,297 + PS417_27835 0.22 -0.5
6,001,298 - PS417_27835 0.22 -1.6
6,001,367 + PS417_27835 0.40 -1.2
6,001,496 - PS417_27835 0.74 -1.5
6,001,496 - +0.1
6,001,496 - PS417_27835 0.74 -1.3
6,001,666 + +0.3
6,001,667 - +0.1
6,001,836 - PS417_27840 0.25 +0.2
6,001,858 - PS417_27840 0.28 +1.1
6,001,860 + PS417_27840 0.28 +0.2
6,001,861 - PS417_27840 0.28 +0.1
6,002,076 + PS417_27840 0.57 +0.4
6,002,077 - PS417_27840 0.57 -1.1
6,002,085 + PS417_27840 0.58 +0.1
6,002,086 - PS417_27840 0.59 +0.3
6,002,086 - PS417_27840 0.59 -0.8
6,002,137 + PS417_27840 0.65 -1.1
6,002,138 - PS417_27840 0.66 +0.7
6,002,138 - PS417_27840 0.66 +0.0
6,002,391 + +0.7
6,002,391 + -0.5
6,002,391 + +1.6
6,002,392 - +0.9
6,002,392 - +1.5
6,002,589 + -0.4

Or see this region's nucleotide sequence