Experiment: 80% Methanol 3 mM; liquid stress
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_27580 and PS417_27585 are separated by 3 nucleotides PS417_27585 and PS417_27590 are separated by 13 nucleotides PS417_27590 and PS417_27595 are separated by 2 nucleotides PS417_27595 and PS417_27600 overlap by 4 nucleotides
PS417_27580: PS417_27580 - diaminopimelate decarboxylase, at 5,949,150 to 5,950,397
_27580
PS417_27585: PS417_27585 - diaminopimelate epimerase, at 5,950,401 to 5,951,231
_27585
PS417_27590: PS417_27590 - hypothetical protein, at 5,951,245 to 5,951,961
_27590
PS417_27595: PS417_27595 - recombinase XerC, at 5,951,964 to 5,952,863
_27595
PS417_27600: PS417_27600 - HAD family hydrolase, at 5,952,860 to 5,953,555
_27600
Position (kb) 5951
5952 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5950.395 kb on + strand at 5950.540 kb on + strand, within PS417_27585 at 5950.540 kb on + strand, within PS417_27585 at 5950.821 kb on + strand, within PS417_27585 at 5951.208 kb on + strand at 5951.243 kb on + strand at 5951.244 kb on - strand at 5951.442 kb on + strand, within PS417_27590 at 5951.442 kb on + strand, within PS417_27590 at 5951.442 kb on + strand, within PS417_27590 at 5951.443 kb on - strand, within PS417_27590 at 5951.445 kb on + strand at 5951.863 kb on + strand, within PS417_27590 at 5951.863 kb on + strand, within PS417_27590 at 5951.864 kb on - strand, within PS417_27590 at 5951.864 kb on - strand, within PS417_27590 at 5951.864 kb on - strand, within PS417_27590 at 5951.896 kb on + strand at 5951.896 kb on + strand at 5951.897 kb on - strand at 5952.384 kb on + strand, within PS417_27595 at 5952.385 kb on - strand, within PS417_27595 at 5952.463 kb on + strand, within PS417_27595 at 5952.464 kb on - strand, within PS417_27595 at 5952.702 kb on - strand, within PS417_27595 at 5952.813 kb on + strand at 5952.865 kb on + strand at 5952.912 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 80% Methanol 3 mM; liquid stress remove 5,950,395 + +0.3 5,950,540 + PS417_27585 0.17 -0.6 5,950,540 + PS417_27585 0.17 +2.9 5,950,821 + PS417_27585 0.51 -1.4 5,951,208 + -0.8 5,951,243 + +1.2 5,951,244 - +0.5 5,951,442 + PS417_27590 0.27 +1.4 5,951,442 + PS417_27590 0.27 +1.4 5,951,442 + PS417_27590 0.27 +0.9 5,951,443 - PS417_27590 0.28 +1.4 5,951,445 + +0.5 5,951,863 + PS417_27590 0.86 +1.1 5,951,863 + PS417_27590 0.86 +1.6 5,951,864 - PS417_27590 0.86 +0.8 5,951,864 - PS417_27590 0.86 +0.9 5,951,864 - PS417_27590 0.86 +1.6 5,951,896 + +0.9 5,951,896 + +1.7 5,951,897 - +0.6 5,952,384 + PS417_27595 0.47 +0.0 5,952,385 - PS417_27595 0.47 -0.1 5,952,463 + PS417_27595 0.55 +0.3 5,952,464 - PS417_27595 0.56 -0.2 5,952,702 - PS417_27595 0.82 +0.0 5,952,813 + -0.3 5,952,865 + -0.3 5,952,912 + -2.2
Or see this region's nucleotide sequence