Experiment: 80% Methanol 3 mM; liquid stress
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_20775 and PS417_20780 are separated by 1 nucleotides PS417_20780 and PS417_20785 are separated by 152 nucleotides PS417_20785 and PS417_20790 are separated by 80 nucleotides
PS417_20775: PS417_20775 - coproporphyrinogen III oxidase, at 4,513,584 to 4,514,966
_20775
PS417_20780: PS417_20780 - hypothetical protein, at 4,514,968 to 4,515,435
_20780
PS417_20785: PS417_20785 - transcriptional regulator, at 4,515,588 to 4,516,322
_20785
PS417_20790: PS417_20790 - adenine phosphoribosyltransferase, at 4,516,403 to 4,516,951
_20790
Position (kb)
4514
4515
4516 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 4514.445 kb on + strand, within PS417_20775 at 4514.532 kb on + strand, within PS417_20775 at 4514.532 kb on + strand, within PS417_20775 at 4514.923 kb on - strand at 4515.073 kb on + strand, within PS417_20780 at 4515.073 kb on + strand, within PS417_20780 at 4515.073 kb on + strand, within PS417_20780 at 4515.073 kb on + strand, within PS417_20780 at 4515.074 kb on - strand, within PS417_20780 at 4515.126 kb on + strand, within PS417_20780 at 4515.127 kb on - strand, within PS417_20780 at 4515.416 kb on + strand at 4515.416 kb on + strand at 4515.417 kb on - strand at 4515.454 kb on + strand at 4515.454 kb on + strand at 4515.455 kb on - strand at 4515.455 kb on - strand at 4515.455 kb on - strand at 4515.458 kb on + strand at 4515.459 kb on - strand at 4515.459 kb on - strand at 4515.459 kb on - strand at 4515.459 kb on - strand at 4515.459 kb on - strand at 4515.459 kb on - strand at 4515.558 kb on + strand at 4515.558 kb on + strand at 4515.558 kb on + strand at 4515.558 kb on + strand at 4515.784 kb on + strand, within PS417_20785 at 4515.784 kb on + strand, within PS417_20785 at 4515.784 kb on + strand, within PS417_20785 at 4516.180 kb on - strand, within PS417_20785 at 4516.269 kb on - strand at 4516.363 kb on + strand at 4516.366 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 80% Methanol 3 mM; liquid stress remove 4,514,445 + PS417_20775 0.62 +1.0 4,514,532 + PS417_20775 0.69 +0.1 4,514,532 + PS417_20775 0.69 -0.7 4,514,923 - -0.5 4,515,073 + PS417_20780 0.22 -1.0 4,515,073 + PS417_20780 0.22 -0.6 4,515,073 + PS417_20780 0.22 +0.0 4,515,073 + PS417_20780 0.22 -0.6 4,515,074 - PS417_20780 0.23 -0.3 4,515,126 + PS417_20780 0.34 -0.7 4,515,127 - PS417_20780 0.34 -1.1 4,515,416 + -0.5 4,515,416 + -0.4 4,515,417 - -0.8 4,515,454 + +0.3 4,515,454 + +0.1 4,515,455 - -0.1 4,515,455 - -0.6 4,515,455 - -0.4 4,515,458 + +0.1 4,515,459 - +0.9 4,515,459 - -0.3 4,515,459 - -0.5 4,515,459 - -0.8 4,515,459 - -1.0 4,515,459 - -0.2 4,515,558 + +1.7 4,515,558 + -0.3 4,515,558 + +0.6 4,515,558 + +0.2 4,515,784 + PS417_20785 0.27 -0.9 4,515,784 + PS417_20785 0.27 -0.9 4,515,784 + PS417_20785 0.27 -0.9 4,516,180 - PS417_20785 0.81 -0.3 4,516,269 - +4.0 4,516,363 + +0.0 4,516,366 - -0.5
Or see this region's nucleotide sequence