Experiment: 80% Methanol 3 mM; liquid stress
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_19415 and PS417_19420 overlap by 4 nucleotides PS417_19420 and PS417_19425 are separated by 41 nucleotides PS417_19425 and PS417_19430 are separated by 124 nucleotides PS417_19430 and PS417_19435 are separated by 51 nucleotides
PS417_19415: PS417_19415 - allantoinase, at 4,210,656 to 4,211,582
_19415
PS417_19420: PS417_19420 - OHCU decarboxylase, at 4,211,579 to 4,212,094
_19420
PS417_19425: PS417_19425 - allantoicase, at 4,212,136 to 4,213,131
_19425
PS417_19430: PS417_19430 - ureidoglycolate hydrolase, at 4,213,256 to 4,213,759
_19430
PS417_19435: PS417_19435 - urate hydroxylase (1.7.3.3) (from data) , at 4,213,811 to 4,215,112
_19435
Position (kb)
4212
4213
4214 Strain fitness (log2 ratio)
-2
-1
0
1 at 4211.146 kb on - strand, within PS417_19415 at 4211.599 kb on + strand at 4211.928 kb on - strand, within PS417_19420 at 4211.928 kb on - strand, within PS417_19420 at 4211.965 kb on - strand, within PS417_19420 at 4211.965 kb on - strand, within PS417_19420 at 4212.061 kb on + strand at 4212.061 kb on + strand at 4212.061 kb on + strand at 4212.062 kb on - strand at 4212.146 kb on - strand at 4212.146 kb on - strand at 4212.589 kb on + strand, within PS417_19425 at 4213.215 kb on - strand at 4213.243 kb on + strand at 4213.609 kb on + strand, within PS417_19430 at 4213.614 kb on - strand, within PS417_19430 at 4213.699 kb on + strand, within PS417_19430 at 4213.757 kb on + strand at 4213.757 kb on + strand at 4213.757 kb on + strand at 4213.804 kb on - strand at 4213.988 kb on + strand, within PS417_19435 at 4213.988 kb on + strand, within PS417_19435 at 4213.989 kb on - strand, within PS417_19435 at 4214.061 kb on - strand, within PS417_19435 at 4214.124 kb on - strand, within PS417_19435 at 4214.124 kb on - strand, within PS417_19435
Per-strain Table
Position Strand Gene LocusTag Fraction 80% Methanol 3 mM; liquid stress remove 4,211,146 - PS417_19415 0.53 +1.3 4,211,599 + -2.0 4,211,928 - PS417_19420 0.68 -0.3 4,211,928 - PS417_19420 0.68 +0.0 4,211,965 - PS417_19420 0.75 -0.4 4,211,965 - PS417_19420 0.75 +0.3 4,212,061 + -0.4 4,212,061 + -2.2 4,212,061 + +0.8 4,212,062 - +1.0 4,212,146 - -0.4 4,212,146 - -0.5 4,212,589 + PS417_19425 0.45 -1.1 4,213,215 - -0.2 4,213,243 + -0.3 4,213,609 + PS417_19430 0.70 +0.2 4,213,614 - PS417_19430 0.71 -0.0 4,213,699 + PS417_19430 0.88 -0.3 4,213,757 + +0.3 4,213,757 + -0.9 4,213,757 + -0.0 4,213,804 - -0.2 4,213,988 + PS417_19435 0.14 +0.1 4,213,988 + PS417_19435 0.14 -0.2 4,213,989 - PS417_19435 0.14 +0.8 4,214,061 - PS417_19435 0.19 +1.2 4,214,124 - PS417_19435 0.24 +0.6 4,214,124 - PS417_19435 0.24 -0.7
Or see this region's nucleotide sequence