Experiment: 80% Methanol 3 mM; solid stress
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_25845 and PS417_25850 are separated by 24 nucleotides PS417_25850 and PS417_25855 overlap by 10 nucleotides PS417_25855 and PS417_25860 are separated by 74 nucleotides PS417_25860 and PS417_25865 are separated by 32 nucleotides
PS417_25845: PS417_25845 - 2', 3'-cyclic nucleotide 2'-phosphodiesterase, at 5,581,548 to 5,582,777
_25845
PS417_25850: PS417_25850 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase, at 5,582,802 to 5,583,320
_25850
PS417_25855: PS417_25855 - dihydroneopterin aldolase, at 5,583,311 to 5,583,664
_25855
PS417_25860: PS417_25860 - glycerol-3-phosphate acyltransferase, at 5,583,739 to 5,584,308
_25860
PS417_25865: PS417_25865 - UGMP family protein, at 5,584,341 to 5,585,366
_25865
Position (kb) 5583
5584 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5582.336 kb on + strand, within PS417_25845 at 5583.297 kb on + strand at 5583.297 kb on + strand at 5583.302 kb on - strand at 5583.705 kb on + strand at 5583.731 kb on + strand at 5583.731 kb on + strand at 5583.731 kb on + strand at 5583.732 kb on - strand at 5583.735 kb on + strand at 5583.735 kb on + strand at 5583.735 kb on + strand at 5583.736 kb on - strand at 5583.766 kb on + strand at 5583.766 kb on + strand at 5583.864 kb on + strand, within PS417_25860 at 5583.864 kb on + strand, within PS417_25860 at 5583.864 kb on + strand, within PS417_25860 at 5583.864 kb on + strand, within PS417_25860 at 5583.865 kb on - strand, within PS417_25860 at 5583.865 kb on - strand, within PS417_25860 at 5583.865 kb on - strand, within PS417_25860 at 5583.929 kb on + strand, within PS417_25860 at 5583.929 kb on + strand, within PS417_25860 at 5583.929 kb on + strand, within PS417_25860 at 5583.992 kb on + strand, within PS417_25860 at 5583.992 kb on + strand, within PS417_25860 at 5583.993 kb on - strand, within PS417_25860 at 5583.993 kb on - strand, within PS417_25860 at 5583.993 kb on - strand, within PS417_25860 at 5584.024 kb on + strand, within PS417_25860 at 5584.025 kb on - strand, within PS417_25860 at 5584.025 kb on - strand at 5584.084 kb on + strand, within PS417_25860 at 5584.085 kb on - strand, within PS417_25860 at 5584.132 kb on + strand, within PS417_25860 at 5584.133 kb on - strand, within PS417_25860 at 5584.306 kb on + strand at 5584.307 kb on - strand at 5584.311 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 80% Methanol 3 mM; solid stress remove 5,582,336 + PS417_25845 0.64 +1.0 5,583,297 + +0.2 5,583,297 + +2.6 5,583,302 - -0.6 5,583,705 + -3.3 5,583,731 + -0.3 5,583,731 + -0.5 5,583,731 + -2.4 5,583,732 - +0.5 5,583,735 + +0.2 5,583,735 + -0.7 5,583,735 + -1.5 5,583,736 - +0.4 5,583,766 + -0.7 5,583,766 + -1.0 5,583,864 + PS417_25860 0.22 -0.2 5,583,864 + PS417_25860 0.22 -1.1 5,583,864 + PS417_25860 0.22 -1.3 5,583,864 + PS417_25860 0.22 +0.3 5,583,865 - PS417_25860 0.22 +0.5 5,583,865 - PS417_25860 0.22 -0.4 5,583,865 - PS417_25860 0.22 +0.2 5,583,929 + PS417_25860 0.33 +2.1 5,583,929 + PS417_25860 0.33 -0.6 5,583,929 + PS417_25860 0.33 -1.7 5,583,992 + PS417_25860 0.44 +0.1 5,583,992 + PS417_25860 0.44 +0.4 5,583,993 - PS417_25860 0.45 +0.3 5,583,993 - PS417_25860 0.45 +0.3 5,583,993 - PS417_25860 0.45 -0.0 5,584,024 + PS417_25860 0.50 +0.0 5,584,025 - PS417_25860 0.50 +1.8 5,584,025 - +1.0 5,584,084 + PS417_25860 0.61 -0.5 5,584,085 - PS417_25860 0.61 +1.8 5,584,132 + PS417_25860 0.69 -0.4 5,584,133 - PS417_25860 0.69 +0.6 5,584,306 + -0.4 5,584,307 - +0.7 5,584,311 - -0.3
Or see this region's nucleotide sequence