Experiment: 80% Methanol 3 mM; liquid stress
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_24770 and PS417_24775 are separated by 177 nucleotides PS417_24775 and PS417_24780 are separated by 105 nucleotides PS417_24780 and PS417_24785 are separated by 29 nucleotides PS417_24785 and PS417_24790 are separated by 138 nucleotides
PS417_24770: PS417_24770 - murein hydrolase transporter LrgA, at 5,372,714 to 5,373,067
_24770
PS417_24775: PS417_24775 - dehydratase, at 5,373,245 to 5,373,700
_24775
PS417_24780: PS417_24780 - peptidase C13, at 5,373,806 to 5,375,524
_24780
PS417_24785: PS417_24785 - nucleoside-diphosphate sugar epimerase, at 5,375,554 to 5,376,195
_24785
PS417_24790: PS417_24790 - hypothetical protein, at 5,376,334 to 5,376,618
_24790
Position (kb)
5373
5374
5375
5376 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5373.060 kb on - strand at 5373.060 kb on - strand at 5373.060 kb on - strand at 5373.060 kb on - strand at 5373.100 kb on + strand at 5373.100 kb on + strand at 5373.100 kb on + strand at 5373.100 kb on + strand at 5373.101 kb on - strand at 5373.101 kb on - strand at 5373.101 kb on - strand at 5373.101 kb on - strand at 5373.116 kb on - strand at 5373.175 kb on + strand at 5373.175 kb on + strand at 5373.176 kb on - strand at 5373.176 kb on - strand at 5373.190 kb on + strand at 5373.214 kb on + strand at 5373.214 kb on + strand at 5373.251 kb on + strand at 5373.252 kb on - strand at 5373.252 kb on - strand at 5373.255 kb on + strand at 5373.255 kb on + strand at 5373.255 kb on + strand at 5373.256 kb on - strand at 5373.256 kb on - strand at 5373.331 kb on + strand, within PS417_24775 at 5373.331 kb on + strand, within PS417_24775 at 5373.510 kb on - strand, within PS417_24775 at 5373.510 kb on - strand, within PS417_24775 at 5373.665 kb on + strand at 5373.665 kb on + strand at 5373.665 kb on + strand at 5373.665 kb on + strand at 5373.665 kb on + strand at 5373.666 kb on - strand at 5373.666 kb on - strand at 5373.666 kb on - strand at 5373.666 kb on - strand at 5373.689 kb on + strand at 5373.723 kb on + strand at 5373.740 kb on + strand at 5373.740 kb on + strand at 5373.741 kb on - strand at 5373.741 kb on - strand at 5373.741 kb on - strand at 5373.744 kb on + strand at 5373.745 kb on - strand at 5373.745 kb on - strand at 5373.751 kb on - strand at 5373.754 kb on + strand at 5373.755 kb on - strand at 5373.755 kb on - strand at 5373.755 kb on - strand at 5373.755 kb on - strand at 5374.385 kb on + strand, within PS417_24780 at 5374.385 kb on + strand, within PS417_24780 at 5374.385 kb on + strand, within PS417_24780 at 5374.385 kb on + strand, within PS417_24780 at 5374.385 kb on + strand, within PS417_24780 at 5374.386 kb on - strand, within PS417_24780 at 5374.386 kb on - strand, within PS417_24780 at 5374.386 kb on - strand, within PS417_24780 at 5374.386 kb on - strand, within PS417_24780 at 5374.523 kb on + strand, within PS417_24780 at 5374.523 kb on + strand, within PS417_24780 at 5374.523 kb on + strand, within PS417_24780 at 5374.524 kb on - strand, within PS417_24780 at 5374.524 kb on - strand, within PS417_24780 at 5374.524 kb on - strand, within PS417_24780 at 5374.593 kb on - strand, within PS417_24780 at 5374.593 kb on - strand, within PS417_24780 at 5374.593 kb on - strand, within PS417_24780 at 5374.811 kb on + strand, within PS417_24780 at 5374.811 kb on + strand, within PS417_24780 at 5374.811 kb on + strand, within PS417_24780 at 5374.812 kb on - strand, within PS417_24780 at 5375.030 kb on - strand, within PS417_24780 at 5375.030 kb on - strand, within PS417_24780 at 5375.033 kb on + strand, within PS417_24780 at 5375.033 kb on + strand, within PS417_24780 at 5375.033 kb on + strand, within PS417_24780 at 5375.034 kb on - strand, within PS417_24780 at 5375.034 kb on - strand at 5375.034 kb on - strand, within PS417_24780 at 5375.285 kb on + strand, within PS417_24780 at 5375.285 kb on + strand at 5375.451 kb on + strand at 5375.500 kb on + strand at 5375.523 kb on - strand at 5375.523 kb on - strand at 5375.523 kb on - strand at 5375.523 kb on - strand at 5375.537 kb on - strand at 5375.557 kb on + strand at 5375.558 kb on - strand at 5375.558 kb on - strand at 5375.574 kb on + strand at 5375.575 kb on - strand at 5375.926 kb on + strand, within PS417_24785 at 5375.927 kb on - strand at 5375.927 kb on - strand, within PS417_24785 at 5376.082 kb on + strand, within PS417_24785 at 5376.193 kb on + strand at 5376.193 kb on + strand at 5376.194 kb on - strand at 5376.194 kb on - strand at 5376.203 kb on + strand at 5376.204 kb on - strand at 5376.272 kb on - strand at 5376.272 kb on - strand at 5376.272 kb on - strand at 5376.272 kb on - strand at 5376.274 kb on + strand at 5376.274 kb on + strand at 5376.274 kb on + strand at 5376.521 kb on + strand, within PS417_24790 at 5376.521 kb on + strand, within PS417_24790 at 5376.521 kb on + strand, within PS417_24790 at 5376.521 kb on + strand, within PS417_24790 at 5376.521 kb on + strand, within PS417_24790 at 5376.522 kb on - strand, within PS417_24790
Per-strain Table
Position Strand Gene LocusTag Fraction 80% Methanol 3 mM; liquid stress remove 5,373,060 - +0.0 5,373,060 - +0.1 5,373,060 - -1.0 5,373,060 - -0.7 5,373,100 + -0.3 5,373,100 + +1.2 5,373,100 + -1.3 5,373,100 + -0.0 5,373,101 - +1.2 5,373,101 - -1.9 5,373,101 - -0.2 5,373,101 - -0.5 5,373,116 - +0.4 5,373,175 + -0.7 5,373,175 + +0.7 5,373,176 - -0.5 5,373,176 - +1.5 5,373,190 + -0.3 5,373,214 + -1.0 5,373,214 + -0.0 5,373,251 + +0.1 5,373,252 - +1.3 5,373,252 - -0.0 5,373,255 + -0.4 5,373,255 + +1.0 5,373,255 + -0.7 5,373,256 - -0.6 5,373,256 - +1.0 5,373,331 + PS417_24775 0.19 -0.2 5,373,331 + PS417_24775 0.19 +0.4 5,373,510 - PS417_24775 0.58 -0.3 5,373,510 - PS417_24775 0.58 -0.5 5,373,665 + -0.6 5,373,665 + -0.5 5,373,665 + +1.0 5,373,665 + +1.9 5,373,665 + -0.3 5,373,666 - -1.4 5,373,666 - -0.2 5,373,666 - +0.1 5,373,666 - -0.2 5,373,689 + +0.2 5,373,723 + +0.4 5,373,740 + -0.4 5,373,740 + +0.2 5,373,741 - +1.2 5,373,741 - -0.5 5,373,741 - -0.0 5,373,744 + +0.2 5,373,745 - +1.2 5,373,745 - +0.3 5,373,751 - -0.6 5,373,754 + -0.2 5,373,755 - -0.2 5,373,755 - +0.3 5,373,755 - +0.3 5,373,755 - +0.2 5,374,385 + PS417_24780 0.34 -0.4 5,374,385 + PS417_24780 0.34 -0.4 5,374,385 + PS417_24780 0.34 -0.8 5,374,385 + PS417_24780 0.34 -0.6 5,374,385 + PS417_24780 0.34 -1.0 5,374,386 - PS417_24780 0.34 +0.1 5,374,386 - PS417_24780 0.34 -1.0 5,374,386 - PS417_24780 0.34 +0.1 5,374,386 - PS417_24780 0.34 -1.7 5,374,523 + PS417_24780 0.42 -0.8 5,374,523 + PS417_24780 0.42 -0.9 5,374,523 + PS417_24780 0.42 -0.3 5,374,524 - PS417_24780 0.42 -0.4 5,374,524 - PS417_24780 0.42 -1.0 5,374,524 - PS417_24780 0.42 -0.5 5,374,593 - PS417_24780 0.46 -1.1 5,374,593 - PS417_24780 0.46 -0.9 5,374,593 - PS417_24780 0.46 -0.6 5,374,811 + PS417_24780 0.58 -0.1 5,374,811 + PS417_24780 0.58 -1.2 5,374,811 + PS417_24780 0.58 -1.8 5,374,812 - PS417_24780 0.59 -1.6 5,375,030 - PS417_24780 0.71 -0.2 5,375,030 - PS417_24780 0.71 -0.5 5,375,033 + PS417_24780 0.71 -0.4 5,375,033 + PS417_24780 0.71 -0.4 5,375,033 + PS417_24780 0.71 -0.3 5,375,034 - PS417_24780 0.71 -1.2 5,375,034 - +1.6 5,375,034 - PS417_24780 0.71 -1.0 5,375,285 + PS417_24780 0.86 -0.7 5,375,285 + -0.5 5,375,451 + -0.4 5,375,500 + +0.8 5,375,523 - +1.1 5,375,523 - -0.2 5,375,523 - +0.7 5,375,523 - +0.1 5,375,537 - -1.7 5,375,557 + -0.6 5,375,558 - -0.3 5,375,558 - -0.2 5,375,574 + -1.0 5,375,575 - +1.4 5,375,926 + PS417_24785 0.58 -1.9 5,375,927 - +0.2 5,375,927 - PS417_24785 0.58 -0.4 5,376,082 + PS417_24785 0.82 +0.1 5,376,193 + -1.2 5,376,193 + +0.8 5,376,194 - +0.1 5,376,194 - -0.3 5,376,203 + -0.9 5,376,204 - -0.0 5,376,272 - +0.7 5,376,272 - +0.4 5,376,272 - -0.4 5,376,272 - -0.2 5,376,274 + -0.3 5,376,274 + -0.6 5,376,274 + +0.4 5,376,521 + PS417_24790 0.66 -0.0 5,376,521 + PS417_24790 0.66 -0.7 5,376,521 + PS417_24790 0.66 +1.4 5,376,521 + PS417_24790 0.66 +1.0 5,376,521 + PS417_24790 0.66 -0.8 5,376,522 - PS417_24790 0.66 +0.2
Or see this region's nucleotide sequence