Experiment: 80% Methanol 3 mM; liquid stress
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_22400 and PS417_22405 are separated by 16 nucleotides PS417_22405 and PS417_22410 are separated by 164 nucleotides PS417_22410 and PS417_22415 are separated by 6 nucleotides PS417_22415 and PS417_22420 are separated by 55 nucleotides
PS417_22400: PS417_22400 - 7-cyano-7-deazaguanine synthase, at 4,853,692 to 4,854,366
_22400
PS417_22405: PS417_22405 - 7-carboxy-7-deazaguanine synthase, at 4,854,383 to 4,855,030
_22405
PS417_22410: PS417_22410 - hypothetical protein, at 4,855,195 to 4,856,028
_22410
PS417_22415: PS417_22415 - peptidoglycan-binding protein, at 4,856,035 to 4,856,535
_22415
PS417_22420: PS417_22420 - translocation protein TolB, at 4,856,591 to 4,857,892
_22420
Position (kb)
4855
4856
4857 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4854.275 kb on + strand, within PS417_22400 at 4854.276 kb on - strand, within PS417_22400 at 4854.276 kb on - strand, within PS417_22400 at 4854.276 kb on - strand, within PS417_22400 at 4854.276 kb on - strand, within PS417_22400 at 4854.276 kb on - strand, within PS417_22400 at 4854.415 kb on + strand at 4854.415 kb on + strand at 4854.415 kb on + strand at 4854.416 kb on - strand at 4854.416 kb on - strand at 4854.416 kb on - strand at 4854.416 kb on - strand at 4854.443 kb on + strand at 4854.512 kb on + strand at 4854.512 kb on + strand, within PS417_22405 at 4854.513 kb on - strand, within PS417_22405 at 4854.513 kb on - strand, within PS417_22405 at 4854.513 kb on - strand, within PS417_22405 at 4854.570 kb on + strand, within PS417_22405 at 4854.571 kb on - strand, within PS417_22405 at 4854.829 kb on - strand, within PS417_22405 at 4854.874 kb on + strand at 4854.875 kb on - strand, within PS417_22405 at 4854.996 kb on + strand at 4855.012 kb on + strand at 4855.012 kb on + strand at 4855.013 kb on - strand at 4855.013 kb on - strand at 4855.016 kb on + strand at 4855.016 kb on + strand at 4855.016 kb on + strand at 4855.016 kb on + strand at 4855.016 kb on + strand at 4855.017 kb on - strand at 4855.017 kb on - strand at 4855.017 kb on - strand at 4855.017 kb on - strand at 4855.030 kb on + strand at 4855.030 kb on + strand at 4855.031 kb on - strand at 4855.052 kb on + strand at 4855.052 kb on + strand at 4855.052 kb on + strand at 4855.053 kb on - strand at 4855.103 kb on + strand at 4855.248 kb on - strand at 4855.309 kb on + strand, within PS417_22410 at 4855.309 kb on + strand, within PS417_22410 at 4855.310 kb on - strand, within PS417_22410 at 4855.310 kb on - strand, within PS417_22410 at 4855.325 kb on + strand, within PS417_22410 at 4855.488 kb on + strand, within PS417_22410 at 4855.548 kb on - strand, within PS417_22410 at 4855.548 kb on - strand, within PS417_22410 at 4855.548 kb on - strand, within PS417_22410 at 4855.550 kb on + strand, within PS417_22410 at 4856.005 kb on + strand at 4856.037 kb on - strand at 4856.041 kb on - strand at 4856.041 kb on - strand at 4856.602 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 80% Methanol 3 mM; liquid stress remove 4,854,275 + PS417_22400 0.86 -1.0 4,854,276 - PS417_22400 0.87 -1.2 4,854,276 - PS417_22400 0.87 -0.4 4,854,276 - PS417_22400 0.87 -1.8 4,854,276 - PS417_22400 0.87 -0.8 4,854,276 - PS417_22400 0.87 -1.3 4,854,415 + +1.2 4,854,415 + -0.5 4,854,415 + -3.1 4,854,416 - +0.2 4,854,416 - -2.5 4,854,416 - -0.2 4,854,416 - -1.8 4,854,443 + -1.8 4,854,512 + +0.8 4,854,512 + PS417_22405 0.20 -1.7 4,854,513 - PS417_22405 0.20 -1.0 4,854,513 - PS417_22405 0.20 -0.1 4,854,513 - PS417_22405 0.20 -1.1 4,854,570 + PS417_22405 0.29 -0.1 4,854,571 - PS417_22405 0.29 -1.1 4,854,829 - PS417_22405 0.69 -1.2 4,854,874 + +0.3 4,854,875 - PS417_22405 0.76 -1.0 4,854,996 + -0.4 4,855,012 + -2.1 4,855,012 + -0.1 4,855,013 - -1.5 4,855,013 - -0.5 4,855,016 + -1.5 4,855,016 + -0.9 4,855,016 + -1.3 4,855,016 + -1.6 4,855,016 + -1.6 4,855,017 - -0.1 4,855,017 - -1.4 4,855,017 - -1.3 4,855,017 - -1.5 4,855,030 + -1.3 4,855,030 + -0.8 4,855,031 - +0.8 4,855,052 + -2.0 4,855,052 + -1.3 4,855,052 + +0.6 4,855,053 - +0.1 4,855,103 + +1.0 4,855,248 - -0.9 4,855,309 + PS417_22410 0.14 -1.7 4,855,309 + PS417_22410 0.14 -0.4 4,855,310 - PS417_22410 0.14 -0.4 4,855,310 - PS417_22410 0.14 -0.1 4,855,325 + PS417_22410 0.16 -0.4 4,855,488 + PS417_22410 0.35 -1.6 4,855,548 - PS417_22410 0.42 -0.5 4,855,548 - PS417_22410 0.42 -1.0 4,855,548 - PS417_22410 0.42 -0.8 4,855,550 + PS417_22410 0.43 -1.7 4,856,005 + +0.5 4,856,037 - +0.5 4,856,041 - -0.4 4,856,041 - -1.9 4,856,602 - +0.3
Or see this region's nucleotide sequence