Experiment: 80% Methanol 2 mM; liquid stress
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_28175 and PS417_28180 are separated by 10 nucleotides PS417_28180 and PS417_28185 are separated by 135 nucleotides PS417_28185 and PS417_28190 are separated by 112 nucleotides PS417_28190 and PS417_28195 overlap by 4 nucleotides
PS417_28175: PS417_28175 - GNAT family acetyltransferase, at 6,084,397 to 6,084,843
_28175
PS417_28180: PS417_28180 - GNAT family acetyltransferase, at 6,084,854 to 6,085,525
_28180
PS417_28185: PS417_28185 - homocysteine methyltransferase, at 6,085,661 to 6,086,557
_28185
PS417_28190: PS417_28190 - amino acid ABC transporter substrate-binding protein, at 6,086,670 to 6,087,491
_28190
PS417_28195: PS417_28195 - amino acid ABC transporter permease, at 6,087,488 to 6,088,318
_28195
Position (kb)
6085
6086
6087 Strain fitness (log2 ratio)
-2
-1
0
1 at 6085.013 kb on + strand, within PS417_28180 at 6085.013 kb on + strand, within PS417_28180 at 6085.014 kb on - strand, within PS417_28180 at 6085.091 kb on + strand, within PS417_28180 at 6085.129 kb on - strand, within PS417_28180 at 6085.242 kb on - strand, within PS417_28180 at 6085.242 kb on - strand, within PS417_28180 at 6085.242 kb on - strand, within PS417_28180 at 6085.256 kb on + strand, within PS417_28180 at 6085.256 kb on + strand, within PS417_28180 at 6085.257 kb on - strand, within PS417_28180 at 6085.257 kb on - strand, within PS417_28180 at 6085.322 kb on + strand, within PS417_28180 at 6085.322 kb on + strand, within PS417_28180 at 6085.523 kb on + strand at 6085.768 kb on - strand, within PS417_28185 at 6086.227 kb on - strand, within PS417_28185 at 6086.379 kb on + strand, within PS417_28185 at 6086.418 kb on + strand, within PS417_28185 at 6086.418 kb on + strand, within PS417_28185 at 6086.418 kb on + strand, within PS417_28185 at 6086.419 kb on - strand, within PS417_28185 at 6086.540 kb on + strand at 6086.540 kb on + strand at 6086.540 kb on + strand at 6086.567 kb on - strand at 6086.615 kb on + strand at 6087.016 kb on - strand, within PS417_28190 at 6087.130 kb on - strand, within PS417_28190 at 6087.137 kb on + strand, within PS417_28190 at 6087.181 kb on - strand, within PS417_28190 at 6087.211 kb on - strand, within PS417_28190 at 6087.348 kb on + strand, within PS417_28190
Per-strain Table
Position Strand Gene LocusTag Fraction 80% Methanol 2 mM; liquid stress remove 6,085,013 + PS417_28180 0.24 -0.5 6,085,013 + PS417_28180 0.24 -0.2 6,085,014 - PS417_28180 0.24 +0.1 6,085,091 + PS417_28180 0.35 +0.0 6,085,129 - PS417_28180 0.41 -0.2 6,085,242 - PS417_28180 0.58 -0.8 6,085,242 - PS417_28180 0.58 +0.9 6,085,242 - PS417_28180 0.58 -0.3 6,085,256 + PS417_28180 0.60 -0.2 6,085,256 + PS417_28180 0.60 -0.5 6,085,257 - PS417_28180 0.60 -0.3 6,085,257 - PS417_28180 0.60 +0.0 6,085,322 + PS417_28180 0.70 +1.2 6,085,322 + PS417_28180 0.70 +0.6 6,085,523 + -0.0 6,085,768 - PS417_28185 0.12 -1.8 6,086,227 - PS417_28185 0.63 -1.1 6,086,379 + PS417_28185 0.80 +0.5 6,086,418 + PS417_28185 0.84 +0.1 6,086,418 + PS417_28185 0.84 -0.5 6,086,418 + PS417_28185 0.84 -1.3 6,086,419 - PS417_28185 0.85 -0.5 6,086,540 + +1.1 6,086,540 + -0.2 6,086,540 + +0.3 6,086,567 - -0.5 6,086,615 + -0.3 6,087,016 - PS417_28190 0.42 -0.8 6,087,130 - PS417_28190 0.56 +0.2 6,087,137 + PS417_28190 0.57 -0.3 6,087,181 - PS417_28190 0.62 +1.3 6,087,211 - PS417_28190 0.66 +0.6 6,087,348 + PS417_28190 0.82 -0.5
Or see this region's nucleotide sequence