Experiment: 80% Methanol 2 mM; liquid stress
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_22565 and PS417_22570 are separated by 350 nucleotides PS417_22570 and PS417_22575 are separated by 89 nucleotides PS417_22575 and PS417_22580 are separated by 167 nucleotides PS417_22580 and PS417_22585 overlap by 4 nucleotides
PS417_22565: PS417_22565 - formyltetrahydrofolate deformylase, at 4,886,301 to 4,887,149
_22565
PS417_22570: PS417_22570 - H-NS histone, at 4,887,500 to 4,887,874
_22570
PS417_22575: PS417_22575 - exonuclease I, at 4,887,964 to 4,889,391
_22575
PS417_22580: PS417_22580 - membrane protein, at 4,889,559 to 4,890,251
_22580
PS417_22585: PS417_22585 - membrane protein, at 4,890,248 to 4,891,228
_22585
Position (kb) 4887
4888
4889
4890 Strain fitness (log2 ratio) -2
-1
0
1 at 4887.227 kb on + strand at 4887.228 kb on - strand at 4887.258 kb on + strand at 4887.261 kb on + strand at 4887.261 kb on + strand at 4887.283 kb on + strand at 4887.286 kb on - strand at 4887.408 kb on - strand at 4887.411 kb on + strand at 4887.427 kb on + strand at 4887.448 kb on + strand at 4887.449 kb on - strand at 4887.451 kb on + strand at 4887.451 kb on + strand at 4887.483 kb on + strand at 4887.483 kb on + strand at 4887.487 kb on + strand at 4887.488 kb on - strand at 4887.539 kb on - strand, within PS417_22570 at 4887.936 kb on + strand at 4887.937 kb on - strand at 4887.974 kb on - strand at 4887.974 kb on - strand at 4887.998 kb on + strand at 4888.087 kb on - strand at 4888.247 kb on - strand, within PS417_22575 at 4888.283 kb on - strand, within PS417_22575 at 4888.316 kb on + strand, within PS417_22575 at 4888.316 kb on + strand, within PS417_22575 at 4888.435 kb on - strand, within PS417_22575 at 4888.514 kb on + strand, within PS417_22575 at 4888.515 kb on - strand, within PS417_22575 at 4888.571 kb on + strand, within PS417_22575 at 4888.572 kb on - strand, within PS417_22575 at 4888.697 kb on + strand, within PS417_22575 at 4888.728 kb on + strand, within PS417_22575 at 4888.729 kb on - strand, within PS417_22575 at 4888.729 kb on - strand, within PS417_22575 at 4888.729 kb on - strand, within PS417_22575 at 4888.729 kb on - strand, within PS417_22575 at 4888.826 kb on - strand, within PS417_22575 at 4889.072 kb on + strand, within PS417_22575 at 4889.073 kb on - strand, within PS417_22575 at 4889.108 kb on + strand, within PS417_22575 at 4889.299 kb on + strand at 4889.299 kb on + strand at 4889.299 kb on + strand at 4889.366 kb on + strand at 4889.397 kb on - strand at 4889.778 kb on + strand, within PS417_22580 at 4889.778 kb on + strand, within PS417_22580 at 4889.779 kb on - strand, within PS417_22580 at 4889.779 kb on - strand, within PS417_22580 at 4890.042 kb on - strand, within PS417_22580 at 4890.042 kb on - strand, within PS417_22580
Per-strain Table
Position Strand Gene LocusTag Fraction 80% Methanol 2 mM; liquid stress remove 4,887,227 + +0.7 4,887,228 - -0.5 4,887,258 + +0.8 4,887,261 + +0.1 4,887,261 + +0.0 4,887,283 + -1.2 4,887,286 - -1.4 4,887,408 - -0.1 4,887,411 + -1.0 4,887,427 + +0.5 4,887,448 + -0.5 4,887,449 - +0.2 4,887,451 + -0.6 4,887,451 + -0.1 4,887,483 + -0.6 4,887,483 + -0.1 4,887,487 + -0.1 4,887,488 - +0.5 4,887,539 - PS417_22570 0.10 -2.1 4,887,936 + -0.1 4,887,937 - +0.8 4,887,974 - +0.5 4,887,974 - +0.4 4,887,998 + -0.2 4,888,087 - -0.9 4,888,247 - PS417_22575 0.20 -0.8 4,888,283 - PS417_22575 0.22 -0.4 4,888,316 + PS417_22575 0.25 -1.0 4,888,316 + PS417_22575 0.25 +1.2 4,888,435 - PS417_22575 0.33 -0.4 4,888,514 + PS417_22575 0.39 +0.2 4,888,515 - PS417_22575 0.39 -0.9 4,888,571 + PS417_22575 0.43 -1.6 4,888,572 - PS417_22575 0.43 -0.6 4,888,697 + PS417_22575 0.51 +0.9 4,888,728 + PS417_22575 0.54 -1.0 4,888,729 - PS417_22575 0.54 -0.7 4,888,729 - PS417_22575 0.54 -1.2 4,888,729 - PS417_22575 0.54 +1.2 4,888,729 - PS417_22575 0.54 +0.6 4,888,826 - PS417_22575 0.60 +0.3 4,889,072 + PS417_22575 0.78 -1.0 4,889,073 - PS417_22575 0.78 -0.7 4,889,108 + PS417_22575 0.80 -0.4 4,889,299 + -0.5 4,889,299 + +0.5 4,889,299 + -1.5 4,889,366 + -0.6 4,889,397 - -0.1 4,889,778 + PS417_22580 0.32 +0.4 4,889,778 + PS417_22580 0.32 +0.6 4,889,779 - PS417_22580 0.32 +0.9 4,889,779 - PS417_22580 0.32 -0.1 4,890,042 - PS417_22580 0.70 +1.2 4,890,042 - PS417_22580 0.70 -0.4
Or see this region's nucleotide sequence