Experiment: 80% Methanol 2 mM; liquid stress
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_06025 and PS417_06030 overlap by 4 nucleotides PS417_06030 and PS417_06035 are separated by 168 nucleotides PS417_06035 and PS417_06040 are separated by 186 nucleotides PS417_06040 and PS417_06045 are separated by 29 nucleotides
PS417_06025: PS417_06025 - alpha/beta hydrolase, at 1,339,405 to 1,340,310
_06025
PS417_06030: PS417_06030 - hypothetical protein, at 1,340,307 to 1,340,633
_06030
PS417_06035: PS417_06035 - hypothetical protein, at 1,340,802 to 1,341,146
_06035
PS417_06040: PS417_06040 - hypothetical protein, at 1,341,333 to 1,341,527
_06040
PS417_06045: PS417_06045 - membrane protein, at 1,341,557 to 1,342,342
_06045
Position (kb)
1340
1341 Strain fitness (log2 ratio)
-2
-1
0
1 at 1339.309 kb on + strand at 1339.309 kb on + strand at 1339.309 kb on + strand at 1339.309 kb on + strand at 1339.309 kb on + strand at 1339.309 kb on + strand at 1339.309 kb on + strand at 1339.310 kb on - strand at 1339.310 kb on - strand at 1339.310 kb on - strand at 1339.310 kb on - strand at 1339.310 kb on - strand at 1339.310 kb on - strand at 1339.310 kb on - strand at 1339.310 kb on - strand at 1339.310 kb on - strand at 1339.385 kb on + strand at 1339.386 kb on - strand at 1339.389 kb on - strand at 1339.433 kb on + strand at 1339.516 kb on + strand, within PS417_06025 at 1339.711 kb on + strand, within PS417_06025 at 1339.751 kb on - strand, within PS417_06025 at 1339.859 kb on - strand, within PS417_06025 at 1339.859 kb on - strand, within PS417_06025 at 1339.938 kb on - strand, within PS417_06025 at 1339.938 kb on - strand, within PS417_06025 at 1340.269 kb on + strand at 1340.270 kb on - strand at 1340.280 kb on + strand at 1340.389 kb on - strand, within PS417_06030 at 1340.389 kb on - strand, within PS417_06030 at 1340.394 kb on + strand, within PS417_06030 at 1340.473 kb on - strand, within PS417_06030 at 1340.473 kb on - strand, within PS417_06030 at 1340.687 kb on + strand at 1340.800 kb on - strand at 1340.800 kb on - strand at 1340.800 kb on - strand at 1340.800 kb on - strand at 1340.800 kb on - strand at 1340.802 kb on - strand at 1340.810 kb on + strand at 1340.915 kb on + strand, within PS417_06035 at 1340.927 kb on + strand, within PS417_06035 at 1341.201 kb on - strand at 1341.201 kb on - strand at 1341.288 kb on + strand at 1341.289 kb on - strand at 1341.289 kb on - strand at 1341.300 kb on - strand at 1341.314 kb on + strand at 1341.314 kb on + strand at 1341.318 kb on + strand at 1341.318 kb on + strand at 1341.319 kb on - strand at 1341.340 kb on + strand at 1341.388 kb on + strand, within PS417_06040 at 1341.388 kb on + strand, within PS417_06040 at 1341.389 kb on - strand, within PS417_06040 at 1341.417 kb on + strand, within PS417_06040 at 1341.526 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 80% Methanol 2 mM; liquid stress remove 1,339,309 + -1.6 1,339,309 + +0.5 1,339,309 + -0.4 1,339,309 + -0.3 1,339,309 + +0.6 1,339,309 + -0.3 1,339,309 + -0.4 1,339,310 - +0.1 1,339,310 - -0.4 1,339,310 - -0.2 1,339,310 - -0.3 1,339,310 - +0.0 1,339,310 - +0.8 1,339,310 - -0.3 1,339,310 - +1.5 1,339,310 - -0.3 1,339,385 + +0.5 1,339,386 - +0.3 1,339,389 - +1.0 1,339,433 + -0.8 1,339,516 + PS417_06025 0.12 +1.0 1,339,711 + PS417_06025 0.34 -0.5 1,339,751 - PS417_06025 0.38 +0.5 1,339,859 - PS417_06025 0.50 -1.2 1,339,859 - PS417_06025 0.50 +0.1 1,339,938 - PS417_06025 0.59 -0.1 1,339,938 - PS417_06025 0.59 +0.9 1,340,269 + -0.4 1,340,270 - -0.1 1,340,280 + -0.4 1,340,389 - PS417_06030 0.25 +0.5 1,340,389 - PS417_06030 0.25 -0.6 1,340,394 + PS417_06030 0.27 -1.4 1,340,473 - PS417_06030 0.51 -0.3 1,340,473 - PS417_06030 0.51 -0.4 1,340,687 + -0.4 1,340,800 - -0.8 1,340,800 - -0.4 1,340,800 - -0.2 1,340,800 - -1.1 1,340,800 - +0.5 1,340,802 - -2.6 1,340,810 + +0.6 1,340,915 + PS417_06035 0.33 +1.2 1,340,927 + PS417_06035 0.36 +0.4 1,341,201 - -0.4 1,341,201 - +0.9 1,341,288 + +0.5 1,341,289 - -0.6 1,341,289 - +0.5 1,341,300 - -0.9 1,341,314 + +0.5 1,341,314 + -0.2 1,341,318 + -0.8 1,341,318 + -1.5 1,341,319 - -0.3 1,341,340 + +0.5 1,341,388 + PS417_06040 0.28 -0.7 1,341,388 + PS417_06040 0.28 +0.5 1,341,389 - PS417_06040 0.29 -0.4 1,341,417 + PS417_06040 0.43 -0.0 1,341,526 - -0.0
Or see this region's nucleotide sequence