Strain Fitness in Escherichia coli BW25113 around b4415

Experiment: 22hr_mediaonly_rep1_lowinoculum

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntybdK and sokE are separated by 350 nucleotidessokE and hokE are separated by 43 nucleotideshokE and insL-2 are separated by 76 nucleotides b0581: ybdK - gamma-glutamyl:cysteine ligase (NCBI), at 605,488 to 606,606 ybdK b4700: sokE - sRNA at remnant mokE/hokE locus (RefSeq), at 606,957 to 607,015 sokE b4415: hokE - toxic polypeptide, small (NCBI), at 607,059 to 607,211 hokE b0582: insL-2 - IS186/IS421 transposase (NCBI), at 607,288 to 608,400 insL-2 Position (kb) 607 608Strain fitness (log2 ratio) -2 -1 0 1at 606.060 kb on + strand, within ybdKat 606.095 kb on + strand, within ybdKat 606.095 kb on + strand, within ybdKat 606.317 kb on - strand, within ybdKat 606.367 kb on - strand, within ybdKat 606.498 kb on + strandat 606.506 kb on - strandat 606.516 kb on - strandat 606.516 kb on - strandat 606.602 kb on + strandat 606.621 kb on - strandat 606.673 kb on + strandat 606.937 kb on + strandat 606.988 kb on + strand, within sokEat 607.102 kb on - strand, within hokEat 607.105 kb on - strand, within hokEat 607.105 kb on - strand, within hokEat 607.122 kb on + strand, within hokEat 607.185 kb on + strand, within hokEat 607.210 kb on - strandat 607.224 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 22hr_mediaonly_rep1_lowinoculum
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606,060 + ybdK b0581 0.51 +0.3
606,095 + ybdK b0581 0.54 +0.9
606,095 + ybdK b0581 0.54 -0.2
606,317 - ybdK b0581 0.74 -1.8
606,367 - ybdK b0581 0.79 -0.6
606,498 + +0.3
606,506 - +0.1
606,516 - +0.5
606,516 - +0.9
606,602 + -0.7
606,621 - -0.0
606,673 + +0.1
606,937 + +1.5
606,988 + sokE b4700 0.53 +0.4
607,102 - hokE b4415 0.28 -0.8
607,105 - hokE b4415 0.30 +0.3
607,105 - hokE b4415 0.30 -0.7
607,122 + hokE b4415 0.41 -0.1
607,185 + hokE b4415 0.82 +0.4
607,210 - -1.2
607,224 + +0.4

Or see this region's nucleotide sequence