Strain Fitness in Escherichia coli BW25113 around b2937

Experiment: 22hr_MSC_rep2_lowinoculum

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyggG and speB are separated by 205 nucleotidesspeB and speA are separated by 137 nucleotides b2936: yggG - orf, hypothetical protein (VIMSS), at 3,079,935 to 3,080,693 yggG b2937: speB - agmatinase (NCBI), at 3,080,899 to 3,081,819 speB b2938: speA - arginine decarboxylase (NCBI), at 3,081,957 to 3,083,933 speA Position (kb) 3080 3081 3082Strain fitness (log2 ratio) -1 0 1 2 3 4at 3079.977 kb on + strandat 3079.985 kb on - strandat 3080.005 kb on + strandat 3080.126 kb on + strand, within yggGat 3080.177 kb on + strand, within yggGat 3080.197 kb on - strand, within yggGat 3080.220 kb on - strand, within yggGat 3080.220 kb on - strand, within yggGat 3080.246 kb on + strand, within yggGat 3080.275 kb on + strand, within yggGat 3080.302 kb on - strand, within yggGat 3080.340 kb on + strand, within yggGat 3080.348 kb on - strand, within yggGat 3080.540 kb on + strand, within yggGat 3080.553 kb on - strand, within yggGat 3080.626 kb on - strandat 3080.683 kb on + strandat 3080.683 kb on + strandat 3080.691 kb on - strandat 3080.691 kb on - strandat 3080.954 kb on + strandat 3080.954 kb on + strandat 3081.056 kb on - strand, within speBat 3081.056 kb on - strand, within speBat 3081.158 kb on + strand, within speBat 3081.193 kb on - strand, within speBat 3081.216 kb on - strand, within speBat 3081.216 kb on - strand, within speBat 3081.263 kb on + strand, within speBat 3081.269 kb on - strand, within speBat 3081.292 kb on + strand, within speBat 3081.312 kb on + strand, within speBat 3081.314 kb on + strand, within speBat 3081.314 kb on + strand, within speBat 3081.314 kb on + strand, within speBat 3081.322 kb on - strand, within speBat 3081.322 kb on - strand, within speBat 3081.322 kb on - strand, within speBat 3081.322 kb on - strand, within speBat 3081.322 kb on - strand, within speBat 3081.347 kb on - strand, within speBat 3081.347 kb on - strand, within speBat 3081.421 kb on + strand, within speBat 3081.421 kb on + strand, within speBat 3081.450 kb on + strand, within speBat 3081.450 kb on + strand, within speBat 3081.540 kb on - strand, within speBat 3081.619 kb on + strand, within speBat 3081.648 kb on + strand, within speBat 3081.648 kb on + strand, within speBat 3081.675 kb on - strand, within speBat 3081.675 kb on - strand, within speBat 3081.708 kb on + strand, within speBat 3081.737 kb on + strandat 3081.804 kb on + strandat 3081.830 kb on + strandat 3081.867 kb on + strandat 3081.867 kb on + strandat 3081.956 kb on - strandat 3081.956 kb on - strandat 3081.957 kb on - strandat 3081.984 kb on - strandat 3082.038 kb on - strandat 3082.102 kb on - strandat 3082.153 kb on - strandat 3082.297 kb on + strand, within speAat 3082.322 kb on - strandat 3082.322 kb on - strand, within speAat 3082.326 kb on - strand, within speAat 3082.326 kb on - strand, within speAat 3082.334 kb on - strandat 3082.337 kb on - strand, within speAat 3082.362 kb on + strand, within speAat 3082.362 kb on - strand, within speAat 3082.370 kb on - strand, within speAat 3082.372 kb on + strand, within speAat 3082.414 kb on + strand, within speAat 3082.422 kb on - strand, within speAat 3082.430 kb on - strand, within speAat 3082.461 kb on - strand, within speAat 3082.652 kb on - strand, within speAat 3082.667 kb on + strand, within speAat 3082.667 kb on + strand, within speAat 3082.742 kb on - strand, within speAat 3082.778 kb on - strand, within speA

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Per-strain Table

Position Strand Gene LocusTag Fraction 22hr_MSC_rep2_lowinoculum
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3,079,977 + -0.5
3,079,985 - +0.2
3,080,005 + -0.0
3,080,126 + yggG b2936 0.25 +0.1
3,080,177 + yggG b2936 0.32 +1.4
3,080,197 - yggG b2936 0.35 +0.5
3,080,220 - yggG b2936 0.38 +0.1
3,080,220 - yggG b2936 0.38 +0.2
3,080,246 + yggG b2936 0.41 +0.2
3,080,275 + yggG b2936 0.45 -0.2
3,080,302 - yggG b2936 0.48 +0.8
3,080,340 + yggG b2936 0.53 +0.2
3,080,348 - yggG b2936 0.54 -0.1
3,080,540 + yggG b2936 0.80 +0.0
3,080,553 - yggG b2936 0.81 +0.4
3,080,626 - -0.5
3,080,683 + +0.5
3,080,683 + -0.2
3,080,691 - +1.3
3,080,691 - -0.3
3,080,954 + -0.1
3,080,954 + +0.3
3,081,056 - speB b2937 0.17 -0.4
3,081,056 - speB b2937 0.17 +1.1
3,081,158 + speB b2937 0.28 +0.0
3,081,193 - speB b2937 0.32 -0.3
3,081,216 - speB b2937 0.34 +0.7
3,081,216 - speB b2937 0.34 -0.4
3,081,263 + speB b2937 0.40 -0.3
3,081,269 - speB b2937 0.40 +0.3
3,081,292 + speB b2937 0.43 +0.1
3,081,312 + speB b2937 0.45 -0.3
3,081,314 + speB b2937 0.45 -0.1
3,081,314 + speB b2937 0.45 +0.2
3,081,314 + speB b2937 0.45 -0.4
3,081,322 - speB b2937 0.46 -1.4
3,081,322 - speB b2937 0.46 -1.7
3,081,322 - speB b2937 0.46 +0.6
3,081,322 - speB b2937 0.46 -1.3
3,081,322 - speB b2937 0.46 -1.1
3,081,347 - speB b2937 0.49 +2.3
3,081,347 - speB b2937 0.49 -0.1
3,081,421 + speB b2937 0.57 -0.1
3,081,421 + speB b2937 0.57 +0.8
3,081,450 + speB b2937 0.60 -1.0
3,081,450 + speB b2937 0.60 -0.5
3,081,540 - speB b2937 0.70 +1.5
3,081,619 + speB b2937 0.78 -0.5
3,081,648 + speB b2937 0.81 -0.3
3,081,648 + speB b2937 0.81 -0.4
3,081,675 - speB b2937 0.84 +0.2
3,081,675 - speB b2937 0.84 +0.5
3,081,708 + speB b2937 0.88 -0.6
3,081,737 + +0.4
3,081,804 + +0.8
3,081,830 + +0.4
3,081,867 + +1.6
3,081,867 + +1.1
3,081,956 - +0.3
3,081,956 - -0.5
3,081,957 - +0.2
3,081,984 - +3.0
3,082,038 - +2.6
3,082,102 - +3.8
3,082,153 - +1.3
3,082,297 + speA b2938 0.17 +2.1
3,082,322 - +1.1
3,082,322 - speA b2938 0.18 +1.4
3,082,326 - speA b2938 0.19 +0.1
3,082,326 - speA b2938 0.19 +1.2
3,082,334 - +0.8
3,082,337 - speA b2938 0.19 +1.5
3,082,362 + speA b2938 0.20 +2.3
3,082,362 - speA b2938 0.20 +1.4
3,082,370 - speA b2938 0.21 +1.7
3,082,372 + speA b2938 0.21 +1.9
3,082,414 + speA b2938 0.23 +1.9
3,082,422 - speA b2938 0.24 +1.6
3,082,430 - speA b2938 0.24 +0.4
3,082,461 - speA b2938 0.25 +1.4
3,082,652 - speA b2938 0.35 +1.3
3,082,667 + speA b2938 0.36 +2.3
3,082,667 + speA b2938 0.36 +2.3
3,082,742 - speA b2938 0.40 +1.2
3,082,778 - speA b2938 0.42 +1.8

Or see this region's nucleotide sequence