Strain Fitness in Escherichia coli BW25113 around b2053

Experiment: 3hr_mediaonly_rep2_lowinoculum

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntwcaH and fcl are separated by 2 nucleotidesfcl and gmd are separated by 2 nucleotidesgmd and wcaF are separated by 25 nucleotideswcaF and wcaE are separated by 15 nucleotides b2051: wcaH - GDP-mannose mannosyl hydrolase (VIMSS), at 2,123,767 to 2,124,246 wcaH b2052: fcl - bifunctional GDP-fucose synthetase: GDP-4-dehydro-6-deoxy-D-mannose epimerase/ GDP-4-dehydro-6-L-deoxygalactose reductase (NCBI), at 2,124,249 to 2,125,214 fcl b2053: gmd - GDP-D-mannose dehydratase, NAD(P)-binding (NCBI), at 2,125,217 to 2,126,338 gmd b2054: wcaF - predicted acyl transferase (NCBI), at 2,126,364 to 2,126,912 wcaF b2055: wcaE - predicted glycosyl transferase (NCBI), at 2,126,928 to 2,127,674 wcaE Position (kb) 2125 2126 2127Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 2124.282 kb on + strandat 2124.282 kb on + strandat 2124.465 kb on + strand, within fclat 2124.601 kb on + strand, within fclat 2124.630 kb on + strand, within fclat 2124.630 kb on + strand, within fclat 2124.722 kb on - strand, within fclat 2124.813 kb on - strand, within fclat 2124.848 kb on - strand, within fclat 2124.896 kb on + strand, within fclat 2124.951 kb on + strand, within fclat 2124.951 kb on + strand, within fclat 2125.046 kb on - strand, within fclat 2125.113 kb on + strand, within fclat 2125.189 kb on + strandat 2125.221 kb on - strandat 2125.556 kb on + strand, within gmdat 2125.566 kb on + strand, within gmdat 2125.570 kb on + strand, within gmdat 2125.570 kb on + strand, within gmdat 2125.643 kb on + strand, within gmdat 2125.706 kb on + strand, within gmdat 2125.707 kb on - strand, within gmdat 2125.797 kb on + strand, within gmdat 2125.797 kb on + strand, within gmdat 2125.828 kb on + strand, within gmdat 2125.887 kb on + strand, within gmdat 2125.941 kb on - strand, within gmdat 2126.048 kb on + strand, within gmdat 2126.090 kb on - strand, within gmdat 2126.093 kb on - strand, within gmdat 2126.151 kb on + strand, within gmdat 2126.181 kb on - strand, within gmdat 2126.225 kb on - strand, within gmdat 2126.225 kb on - strand, within gmdat 2126.247 kb on - strandat 2126.247 kb on - strandat 2126.247 kb on - strandat 2126.267 kb on + strandat 2126.388 kb on + strandat 2126.398 kb on - strandat 2126.451 kb on - strandat 2126.451 kb on - strand, within wcaFat 2126.456 kb on - strand, within wcaFat 2126.456 kb on - strand, within wcaFat 2126.498 kb on + strand, within wcaFat 2126.515 kb on + strand, within wcaFat 2126.550 kb on - strand, within wcaFat 2126.550 kb on - strand, within wcaFat 2126.562 kb on + strand, within wcaFat 2126.562 kb on - strand, within wcaFat 2126.562 kb on - strand, within wcaFat 2126.577 kb on + strand, within wcaFat 2126.577 kb on + strand, within wcaFat 2126.584 kb on - strand, within wcaFat 2126.587 kb on + strand, within wcaFat 2126.614 kb on + strand, within wcaFat 2126.616 kb on + strand, within wcaFat 2126.621 kb on + strand, within wcaFat 2126.629 kb on + strand, within wcaFat 2126.660 kb on - strand, within wcaFat 2126.681 kb on + strand, within wcaFat 2126.681 kb on + strand, within wcaFat 2126.914 kb on + strandat 2127.243 kb on - strand, within wcaE

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Per-strain Table

Position Strand Gene LocusTag Fraction 3hr_mediaonly_rep2_lowinoculum
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2,124,282 + -0.6
2,124,282 + +0.4
2,124,465 + fcl b2052 0.22 -1.3
2,124,601 + fcl b2052 0.36 -0.4
2,124,630 + fcl b2052 0.39 -3.0
2,124,630 + fcl b2052 0.39 +1.1
2,124,722 - fcl b2052 0.49 -1.4
2,124,813 - fcl b2052 0.58 +1.1
2,124,848 - fcl b2052 0.62 -0.3
2,124,896 + fcl b2052 0.67 -0.9
2,124,951 + fcl b2052 0.73 +0.6
2,124,951 + fcl b2052 0.73 +0.1
2,125,046 - fcl b2052 0.83 +1.1
2,125,113 + fcl b2052 0.89 -0.2
2,125,189 + -0.2
2,125,221 - -0.9
2,125,556 + gmd b2053 0.30 -0.2
2,125,566 + gmd b2053 0.31 -0.3
2,125,570 + gmd b2053 0.31 +0.4
2,125,570 + gmd b2053 0.31 -0.5
2,125,643 + gmd b2053 0.38 +0.5
2,125,706 + gmd b2053 0.44 -1.8
2,125,707 - gmd b2053 0.44 +0.1
2,125,797 + gmd b2053 0.52 +0.4
2,125,797 + gmd b2053 0.52 -2.2
2,125,828 + gmd b2053 0.54 -1.0
2,125,887 + gmd b2053 0.60 +0.1
2,125,941 - gmd b2053 0.65 +0.3
2,126,048 + gmd b2053 0.74 -0.6
2,126,090 - gmd b2053 0.78 +0.5
2,126,093 - gmd b2053 0.78 -0.8
2,126,151 + gmd b2053 0.83 -1.4
2,126,181 - gmd b2053 0.86 -1.2
2,126,225 - gmd b2053 0.90 -1.3
2,126,225 - gmd b2053 0.90 -2.2
2,126,247 - +0.2
2,126,247 - +1.3
2,126,247 - +0.1
2,126,267 + +0.2
2,126,388 + +0.6
2,126,398 - -0.3
2,126,451 - -0.1
2,126,451 - wcaF b2054 0.16 -2.1
2,126,456 - wcaF b2054 0.17 -1.9
2,126,456 - wcaF b2054 0.17 +0.4
2,126,498 + wcaF b2054 0.24 -0.9
2,126,515 + wcaF b2054 0.28 +0.9
2,126,550 - wcaF b2054 0.34 -2.1
2,126,550 - wcaF b2054 0.34 -0.1
2,126,562 + wcaF b2054 0.36 +0.4
2,126,562 - wcaF b2054 0.36 -3.2
2,126,562 - wcaF b2054 0.36 +0.7
2,126,577 + wcaF b2054 0.39 -1.5
2,126,577 + wcaF b2054 0.39 +0.9
2,126,584 - wcaF b2054 0.40 -1.1
2,126,587 + wcaF b2054 0.41 -1.3
2,126,614 + wcaF b2054 0.46 +0.7
2,126,616 + wcaF b2054 0.46 -0.8
2,126,621 + wcaF b2054 0.47 -4.0
2,126,629 + wcaF b2054 0.48 +0.3
2,126,660 - wcaF b2054 0.54 +1.7
2,126,681 + wcaF b2054 0.58 -0.5
2,126,681 + wcaF b2054 0.58 -1.9
2,126,914 + +2.7
2,127,243 - wcaE b2055 0.42 +2.7

Or see this region's nucleotide sequence