Experiment: 3hr_mediaonly_rep2_lowinoculum
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt wcaL and wcaK overlap by 4 nucleotides wcaK and wzxC are separated by 275 nucleotides
b2044: wcaL - predicted glycosyl transferase (NCBI), at 2,113,931 to 2,115,151
wcaL
b2045: wcaK - predicted pyruvyl transferase (NCBI), at 2,115,148 to 2,116,428
wcaK
b2046: wzxC - colanic acid exporter (NCBI), at 2,116,704 to 2,118,182
wzxC
Position (kb)
2115
2116
2117 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2114.251 kb on - strand, within wcaL at 2114.539 kb on - strand, within wcaL at 2114.789 kb on - strand, within wcaL at 2114.874 kb on - strand, within wcaL at 2115.177 kb on - strand at 2115.320 kb on + strand, within wcaK at 2115.414 kb on - strand, within wcaK at 2115.426 kb on - strand, within wcaK at 2115.426 kb on - strand, within wcaK at 2115.441 kb on + strand, within wcaK at 2115.507 kb on - strand, within wcaK at 2115.528 kb on + strand, within wcaK at 2115.548 kb on + strand, within wcaK at 2115.584 kb on + strand, within wcaK at 2115.585 kb on - strand, within wcaK at 2115.603 kb on - strand, within wcaK at 2115.651 kb on + strand, within wcaK at 2115.660 kb on + strand, within wcaK at 2115.708 kb on + strand, within wcaK at 2115.716 kb on - strand, within wcaK at 2115.754 kb on - strand, within wcaK at 2115.790 kb on + strand, within wcaK at 2115.941 kb on + strand, within wcaK at 2115.941 kb on + strand, within wcaK at 2115.949 kb on - strand, within wcaK at 2115.959 kb on - strand, within wcaK at 2115.959 kb on - strand, within wcaK at 2115.968 kb on + strand, within wcaK at 2115.968 kb on + strand, within wcaK at 2115.976 kb on - strand, within wcaK at 2115.995 kb on + strand, within wcaK at 2116.108 kb on + strand, within wcaK at 2116.108 kb on + strand, within wcaK at 2116.413 kb on + strand at 2116.513 kb on - strand at 2116.513 kb on - strand at 2116.566 kb on - strand at 2116.566 kb on - strand at 2116.604 kb on - strand at 2116.868 kb on + strand, within wzxC at 2116.868 kb on + strand, within wzxC at 2116.880 kb on + strand, within wzxC at 2116.888 kb on - strand, within wzxC at 2116.888 kb on - strand, within wzxC at 2116.925 kb on + strand, within wzxC at 2117.172 kb on - strand, within wzxC at 2117.175 kb on + strand, within wzxC at 2117.196 kb on - strand, within wzxC at 2117.246 kb on + strand, within wzxC at 2117.262 kb on - strand, within wzxC at 2117.262 kb on - strand, within wzxC at 2117.272 kb on + strand, within wzxC at 2117.301 kb on + strand, within wzxC
Per-strain Table
Position Strand Gene LocusTag Fraction 3hr_mediaonly_rep2_lowinoculum remove 2,114,251 - wcaL b2044 0.26 -0.5 2,114,539 - wcaL b2044 0.50 -1.1 2,114,789 - wcaL b2044 0.70 -0.1 2,114,874 - wcaL b2044 0.77 -0.9 2,115,177 - -1.3 2,115,320 + wcaK b2045 0.13 -0.4 2,115,414 - wcaK b2045 0.21 -1.0 2,115,426 - wcaK b2045 0.22 -0.0 2,115,426 - wcaK b2045 0.22 -1.9 2,115,441 + wcaK b2045 0.23 -1.2 2,115,507 - wcaK b2045 0.28 +0.1 2,115,528 + wcaK b2045 0.30 +1.3 2,115,548 + wcaK b2045 0.31 -0.1 2,115,584 + wcaK b2045 0.34 -0.2 2,115,585 - wcaK b2045 0.34 +0.0 2,115,603 - wcaK b2045 0.36 -0.1 2,115,651 + wcaK b2045 0.39 -0.5 2,115,660 + wcaK b2045 0.40 -0.8 2,115,708 + wcaK b2045 0.44 -1.2 2,115,716 - wcaK b2045 0.44 +0.3 2,115,754 - wcaK b2045 0.47 +0.1 2,115,790 + wcaK b2045 0.50 -2.0 2,115,941 + wcaK b2045 0.62 -0.8 2,115,941 + wcaK b2045 0.62 +1.3 2,115,949 - wcaK b2045 0.63 +0.4 2,115,959 - wcaK b2045 0.63 -1.1 2,115,959 - wcaK b2045 0.63 -0.8 2,115,968 + wcaK b2045 0.64 +0.2 2,115,968 + wcaK b2045 0.64 -0.2 2,115,976 - wcaK b2045 0.65 -1.7 2,115,995 + wcaK b2045 0.66 -0.6 2,116,108 + wcaK b2045 0.75 -1.3 2,116,108 + wcaK b2045 0.75 -0.9 2,116,413 + -0.8 2,116,513 - +0.3 2,116,513 - +0.1 2,116,566 - +1.5 2,116,566 - -0.1 2,116,604 - +0.4 2,116,868 + wzxC b2046 0.11 +2.7 2,116,868 + wzxC b2046 0.11 -0.2 2,116,880 + wzxC b2046 0.12 -0.5 2,116,888 - wzxC b2046 0.12 -0.7 2,116,888 - wzxC b2046 0.12 -0.4 2,116,925 + wzxC b2046 0.15 -3.3 2,117,172 - wzxC b2046 0.32 +0.1 2,117,175 + wzxC b2046 0.32 -2.4 2,117,196 - wzxC b2046 0.33 +0.8 2,117,246 + wzxC b2046 0.37 -0.6 2,117,262 - wzxC b2046 0.38 -0.1 2,117,262 - wzxC b2046 0.38 -2.4 2,117,272 + wzxC b2046 0.38 +0.6 2,117,301 + wzxC b2046 0.40 +0.3
Or see this region's nucleotide sequence