Strain Fitness in Escherichia coli BW25113 around b1065

Experiment: 3hr_MSC_rep2_lowinoculum

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyceB and grxB are separated by 133 nucleotidesgrxB and yceL are separated by 63 nucleotidesyceL and rimJ are separated by 235 nucleotidesrimJ and yceH are separated by 10 nucleotides b1063: yceB - predicted lipoprotein (NCBI), at 1,121,936 to 1,122,496 yceB b1064: grxB - glutaredoxin 2 (Grx2) (NCBI), at 1,122,630 to 1,123,277 grxB b1065: yceL - orf, hypothetical protein (VIMSS), at 1,123,341 to 1,124,549 yceL b1066: rimJ - ribosomal-protein-S5-alanine N-acetyltransferase (NCBI), at 1,124,785 to 1,125,369 rimJ b1067: yceH - hypothetical protein (NCBI), at 1,125,380 to 1,126,027 yceH Position (kb) 1123 1124 1125Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 1122.356 kb on + strand, within yceBat 1122.356 kb on + strand, within yceBat 1122.501 kb on + strandat 1122.533 kb on + strandat 1122.546 kb on - strandat 1122.549 kb on - strandat 1122.556 kb on + strandat 1122.689 kb on + strandat 1122.829 kb on - strand, within grxBat 1122.942 kb on - strand, within grxBat 1123.018 kb on - strand, within grxBat 1123.099 kb on + strand, within grxBat 1123.099 kb on + strand, within grxBat 1123.099 kb on + strand, within grxBat 1123.195 kb on - strand, within grxBat 1123.274 kb on + strandat 1123.322 kb on + strandat 1123.322 kb on + strandat 1123.329 kb on - strandat 1123.443 kb on + strandat 1123.891 kb on - strand, within yceLat 1123.895 kb on - strand, within yceLat 1123.978 kb on - strand, within yceLat 1124.118 kb on + strand, within yceLat 1124.126 kb on - strand, within yceLat 1124.164 kb on - strand, within yceLat 1124.215 kb on - strand, within yceLat 1124.329 kb on - strand, within yceLat 1124.396 kb on + strand, within yceLat 1124.427 kb on + strand, within yceLat 1124.427 kb on + strand, within yceLat 1124.715 kb on + strandat 1124.723 kb on + strandat 1124.731 kb on + strandat 1124.737 kb on - strandat 1124.739 kb on - strandat 1124.744 kb on - strandat 1124.750 kb on + strandat 1124.773 kb on + strandat 1124.773 kb on + strandat 1124.785 kb on - strandat 1124.785 kb on - strandat 1124.785 kb on - strandat 1124.786 kb on - strandat 1124.822 kb on + strandat 1124.825 kb on + strandat 1124.847 kb on - strand, within rimJat 1124.912 kb on + strand, within rimJat 1124.912 kb on + strand, within rimJat 1124.922 kb on - strand, within rimJat 1124.954 kb on - strand, within rimJat 1124.965 kb on + strand, within rimJat 1125.088 kb on + strand, within rimJat 1125.142 kb on - strand, within rimJat 1125.142 kb on - strand, within rimJat 1125.157 kb on - strand, within rimJat 1125.169 kb on + strand, within rimJat 1125.173 kb on + strand, within rimJat 1125.181 kb on - strand, within rimJat 1125.184 kb on - strand, within rimJat 1125.236 kb on - strand, within rimJat 1125.289 kb on - strand, within rimJat 1125.289 kb on - strand, within rimJat 1125.352 kb on - strandat 1125.407 kb on - strandat 1125.468 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 3hr_MSC_rep2_lowinoculum
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1,122,356 + yceB b1063 0.75 -3.3
1,122,356 + yceB b1063 0.75 -3.0
1,122,501 + +0.2
1,122,533 + -0.7
1,122,546 - +0.1
1,122,549 - -0.2
1,122,556 + -0.2
1,122,689 + -0.5
1,122,829 - grxB b1064 0.31 +0.5
1,122,942 - grxB b1064 0.48 -0.1
1,123,018 - grxB b1064 0.60 -2.8
1,123,099 + grxB b1064 0.72 +0.6
1,123,099 + grxB b1064 0.72 -0.7
1,123,099 + grxB b1064 0.72 -1.2
1,123,195 - grxB b1064 0.87 -0.1
1,123,274 + -1.4
1,123,322 + +0.8
1,123,322 + +0.5
1,123,329 - +1.3
1,123,443 + +0.4
1,123,891 - yceL b1065 0.45 +0.1
1,123,895 - yceL b1065 0.46 -0.6
1,123,978 - yceL b1065 0.53 -2.2
1,124,118 + yceL b1065 0.64 -2.8
1,124,126 - yceL b1065 0.65 -0.0
1,124,164 - yceL b1065 0.68 +0.0
1,124,215 - yceL b1065 0.72 -0.5
1,124,329 - yceL b1065 0.82 -1.2
1,124,396 + yceL b1065 0.87 -2.7
1,124,427 + yceL b1065 0.90 -2.1
1,124,427 + yceL b1065 0.90 +0.3
1,124,715 + -0.6
1,124,723 + +0.0
1,124,731 + +0.7
1,124,737 - -0.0
1,124,739 - +0.7
1,124,744 - -0.1
1,124,750 + -0.4
1,124,773 + +0.8
1,124,773 + -1.5
1,124,785 - +1.3
1,124,785 - +1.9
1,124,785 - -0.2
1,124,786 - -0.2
1,124,822 + -2.2
1,124,825 + -2.1
1,124,847 - rimJ b1066 0.11 -1.1
1,124,912 + rimJ b1066 0.22 +0.6
1,124,912 + rimJ b1066 0.22 +0.4
1,124,922 - rimJ b1066 0.23 -1.7
1,124,954 - rimJ b1066 0.29 -0.8
1,124,965 + rimJ b1066 0.31 -0.2
1,125,088 + rimJ b1066 0.52 -0.6
1,125,142 - rimJ b1066 0.61 -0.9
1,125,142 - rimJ b1066 0.61 -0.1
1,125,157 - rimJ b1066 0.64 -2.8
1,125,169 + rimJ b1066 0.66 +0.8
1,125,173 + rimJ b1066 0.66 -1.8
1,125,181 - rimJ b1066 0.68 +0.4
1,125,184 - rimJ b1066 0.68 -0.0
1,125,236 - rimJ b1066 0.77 -1.2
1,125,289 - rimJ b1066 0.86 -0.1
1,125,289 - rimJ b1066 0.86 +0.1
1,125,352 - -0.2
1,125,407 - -3.8
1,125,468 + -0.2

Or see this region's nucleotide sequence