Experiment: 3hr_MSC_rep2_lowinoculum
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybiI and ybiX are separated by 73 nucleotides ybiX and fiu are separated by 41 nucleotides fiu and ybiM are separated by 264 nucleotides ybiM and ybiN are separated by 275 nucleotides
b0803: ybiI - hypothetical protein (NCBI), at 837,413 to 837,679
ybiI
b0804: ybiX - putative enzyme (VIMSS), at 837,753 to 838,430
ybiX
b0805: fiu - predicted iron outer membrane transporter (NCBI), at 838,472 to 840,754
fiu
b0806: ybiM - orf, hypothetical protein (VIMSS), at 841,019 to 841,279
ybiM
b0807: ybiN - orf, hypothetical protein (VIMSS), at 841,555 to 842,481
ybiN
Position (kb) 838
839
840
841 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 837.550 kb on - strand, within ybiI at 837.567 kb on - strand, within ybiI at 837.576 kb on - strand, within ybiI at 837.630 kb on + strand, within ybiI at 837.638 kb on - strand, within ybiI at 837.723 kb on + strand at 837.800 kb on + strand at 837.853 kb on - strand, within ybiX at 838.096 kb on + strand, within ybiX at 838.096 kb on + strand, within ybiX at 838.140 kb on - strand, within ybiX at 838.140 kb on - strand, within ybiX at 838.195 kb on - strand, within ybiX at 838.195 kb on - strand, within ybiX at 838.195 kb on - strand, within ybiX at 838.253 kb on - strand, within ybiX at 838.511 kb on - strand at 838.511 kb on - strand at 838.519 kb on + strand at 838.563 kb on + strand at 838.596 kb on - strand at 838.790 kb on - strand, within fiu at 838.790 kb on - strand, within fiu at 838.790 kb on - strand, within fiu at 838.797 kb on + strand, within fiu at 838.800 kb on - strand, within fiu at 838.800 kb on - strand, within fiu at 838.983 kb on + strand, within fiu at 839.091 kb on + strand, within fiu at 839.110 kb on + strand, within fiu at 839.230 kb on + strand, within fiu at 839.507 kb on + strand, within fiu at 839.772 kb on - strand, within fiu at 839.772 kb on - strand, within fiu at 839.778 kb on - strand, within fiu at 839.793 kb on - strand, within fiu at 839.934 kb on - strand, within fiu at 840.054 kb on - strand, within fiu at 840.279 kb on + strand, within fiu at 840.279 kb on + strand, within fiu at 840.315 kb on + strand, within fiu at 840.315 kb on + strand, within fiu at 840.323 kb on - strand, within fiu at 840.323 kb on - strand, within fiu at 840.323 kb on - strand, within fiu at 840.324 kb on - strand, within fiu at 840.324 kb on - strand, within fiu at 840.361 kb on + strand, within fiu at 840.361 kb on + strand, within fiu at 840.380 kb on - strand, within fiu at 840.436 kb on - strand, within fiu at 840.436 kb on - strand, within fiu at 840.483 kb on - strand, within fiu at 840.539 kb on + strand at 840.539 kb on + strand at 840.820 kb on + strand at 840.832 kb on - strand at 840.832 kb on - strand at 841.192 kb on + strand, within ybiM at 841.223 kb on - strand, within ybiM at 841.327 kb on + strand at 841.327 kb on + strand at 841.546 kb on - strand at 841.559 kb on - strand at 841.559 kb on - strand at 841.559 kb on - strand at 841.658 kb on + strand, within ybiN at 841.678 kb on - strand, within ybiN at 841.682 kb on + strand, within ybiN at 841.689 kb on - strand, within ybiN
Per-strain Table
Position Strand Gene LocusTag Fraction 3hr_MSC_rep2_lowinoculum remove 837,550 - ybiI b0803 0.51 -0.6 837,567 - ybiI b0803 0.58 -0.3 837,576 - ybiI b0803 0.61 -3.5 837,630 + ybiI b0803 0.81 +1.1 837,638 - ybiI b0803 0.84 +0.4 837,723 + -0.9 837,800 + +0.6 837,853 - ybiX b0804 0.15 -1.8 838,096 + ybiX b0804 0.51 +0.8 838,096 + ybiX b0804 0.51 -1.7 838,140 - ybiX b0804 0.57 +0.9 838,140 - ybiX b0804 0.57 +0.4 838,195 - ybiX b0804 0.65 -0.4 838,195 - ybiX b0804 0.65 -1.3 838,195 - ybiX b0804 0.65 -0.4 838,253 - ybiX b0804 0.74 -0.0 838,511 - -1.8 838,511 - +0.1 838,519 + +0.4 838,563 + -0.3 838,596 - +0.1 838,790 - fiu b0805 0.14 -1.6 838,790 - fiu b0805 0.14 +0.1 838,790 - fiu b0805 0.14 -0.5 838,797 + fiu b0805 0.14 -3.5 838,800 - fiu b0805 0.14 -2.0 838,800 - fiu b0805 0.14 -2.2 838,983 + fiu b0805 0.22 -0.9 839,091 + fiu b0805 0.27 +0.3 839,110 + fiu b0805 0.28 -1.7 839,230 + fiu b0805 0.33 +1.2 839,507 + fiu b0805 0.45 -0.7 839,772 - fiu b0805 0.57 +0.8 839,772 - fiu b0805 0.57 +0.4 839,778 - fiu b0805 0.57 -1.9 839,793 - fiu b0805 0.58 -0.8 839,934 - fiu b0805 0.64 -3.6 840,054 - fiu b0805 0.69 -0.0 840,279 + fiu b0805 0.79 -0.7 840,279 + fiu b0805 0.79 -1.2 840,315 + fiu b0805 0.81 +0.4 840,315 + fiu b0805 0.81 +0.4 840,323 - fiu b0805 0.81 +0.2 840,323 - fiu b0805 0.81 -0.2 840,323 - fiu b0805 0.81 -1.2 840,324 - fiu b0805 0.81 -2.6 840,324 - fiu b0805 0.81 +0.5 840,361 + fiu b0805 0.83 -1.6 840,361 + fiu b0805 0.83 +0.5 840,380 - fiu b0805 0.84 -1.1 840,436 - fiu b0805 0.86 -0.1 840,436 - fiu b0805 0.86 -0.5 840,483 - fiu b0805 0.88 -0.6 840,539 + -1.3 840,539 + +0.3 840,820 + -0.2 840,832 - -0.6 840,832 - -1.7 841,192 + ybiM b0806 0.66 -0.7 841,223 - ybiM b0806 0.78 -0.1 841,327 + +1.1 841,327 + -1.3 841,546 - +0.3 841,559 - +0.0 841,559 - -0.4 841,559 - +0.5 841,658 + ybiN b0807 0.11 -3.5 841,678 - ybiN b0807 0.13 +0.4 841,682 + ybiN b0807 0.14 +0.6 841,689 - ybiN b0807 0.14 +0.5
Or see this region's nucleotide sequence