Experiment: 3hr_MSC_rep2_lowinoculum
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lipA and ybeF are separated by 208 nucleotides ybeF and lipB are separated by 258 nucleotides
b0628: lipA - lipoyl synthase (NCBI), at 658,474 to 659,439
lipA
b0629: ybeF - putative transcriptional regulator LYSR-type (VIMSS), at 659,648 to 660,601
ybeF
b0630: lipB - protein of lipoate biosynthesis (VIMSS), at 660,860 to 661,501
lipB
Position (kb) 659
660
661 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 659.441 kb on - strand at 659.567 kb on - strand at 659.567 kb on - strand at 659.630 kb on + strand at 659.689 kb on - strand at 659.689 kb on - strand at 659.699 kb on - strand at 659.786 kb on + strand, within ybeF at 660.067 kb on + strand, within ybeF at 660.067 kb on - strand, within ybeF at 660.116 kb on - strand, within ybeF at 660.209 kb on + strand, within ybeF at 660.233 kb on + strand, within ybeF at 660.260 kb on + strand, within ybeF at 660.260 kb on + strand, within ybeF at 660.273 kb on + strand, within ybeF at 660.281 kb on - strand, within ybeF at 660.281 kb on - strand, within ybeF at 660.298 kb on + strand, within ybeF at 660.298 kb on + strand, within ybeF at 660.308 kb on - strand, within ybeF at 660.310 kb on + strand, within ybeF at 660.317 kb on + strand, within ybeF at 660.322 kb on + strand, within ybeF at 660.326 kb on - strand, within ybeF at 660.327 kb on - strand, within ybeF at 660.327 kb on - strand, within ybeF at 660.330 kb on - strand, within ybeF at 660.343 kb on + strand, within ybeF at 660.343 kb on + strand, within ybeF at 660.349 kb on + strand, within ybeF at 660.350 kb on + strand, within ybeF at 660.351 kb on + strand, within ybeF at 660.351 kb on - strand, within ybeF at 660.351 kb on - strand, within ybeF at 660.354 kb on + strand, within ybeF at 660.354 kb on + strand, within ybeF at 660.356 kb on + strand, within ybeF at 660.356 kb on + strand, within ybeF at 660.359 kb on - strand, within ybeF at 660.359 kb on - strand, within ybeF at 660.364 kb on - strand, within ybeF at 660.401 kb on - strand, within ybeF at 660.401 kb on - strand, within ybeF at 660.403 kb on + strand, within ybeF at 660.403 kb on + strand, within ybeF at 660.403 kb on + strand, within ybeF at 660.403 kb on + strand, within ybeF at 660.411 kb on - strand, within ybeF at 660.417 kb on - strand, within ybeF at 660.420 kb on + strand, within ybeF at 660.432 kb on - strand, within ybeF at 660.439 kb on + strand, within ybeF at 660.476 kb on + strand, within ybeF at 660.476 kb on + strand, within ybeF at 660.476 kb on + strand, within ybeF at 660.476 kb on + strand, within ybeF at 660.479 kb on + strand, within ybeF at 660.479 kb on + strand, within ybeF at 660.505 kb on + strand, within ybeF at 660.505 kb on + strand, within ybeF at 660.506 kb on + strand at 660.527 kb on + strand at 660.543 kb on + strand at 660.543 kb on + strand at 660.552 kb on + strand at 660.596 kb on + strand at 660.730 kb on - strand at 660.850 kb on - strand at 660.850 kb on - strand at 661.084 kb on - strand, within lipB at 661.146 kb on + strand, within lipB at 661.264 kb on + strand, within lipB at 661.509 kb on + strand at 661.519 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 3hr_MSC_rep2_lowinoculum remove 659,441 - -2.4 659,567 - -1.9 659,567 - +0.9 659,630 + +0.5 659,689 - +0.5 659,689 - +0.3 659,699 - +0.0 659,786 + ybeF b0629 0.14 -0.2 660,067 + ybeF b0629 0.44 -1.0 660,067 - ybeF b0629 0.44 -1.4 660,116 - ybeF b0629 0.49 -0.1 660,209 + ybeF b0629 0.59 -1.8 660,233 + ybeF b0629 0.61 -0.4 660,260 + ybeF b0629 0.64 -0.6 660,260 + ybeF b0629 0.64 -0.6 660,273 + ybeF b0629 0.66 -2.9 660,281 - ybeF b0629 0.66 -1.4 660,281 - ybeF b0629 0.66 -0.4 660,298 + ybeF b0629 0.68 -0.8 660,298 + ybeF b0629 0.68 +0.2 660,308 - ybeF b0629 0.69 +0.3 660,310 + ybeF b0629 0.69 -0.3 660,317 + ybeF b0629 0.70 +0.1 660,322 + ybeF b0629 0.71 +1.1 660,326 - ybeF b0629 0.71 -1.0 660,327 - ybeF b0629 0.71 +0.6 660,327 - ybeF b0629 0.71 -2.1 660,330 - ybeF b0629 0.71 -0.1 660,343 + ybeF b0629 0.73 -1.1 660,343 + ybeF b0629 0.73 -1.5 660,349 + ybeF b0629 0.73 +1.1 660,350 + ybeF b0629 0.74 -2.7 660,351 + ybeF b0629 0.74 +0.2 660,351 - ybeF b0629 0.74 -1.5 660,351 - ybeF b0629 0.74 -1.2 660,354 + ybeF b0629 0.74 +0.4 660,354 + ybeF b0629 0.74 -3.6 660,356 + ybeF b0629 0.74 -1.1 660,356 + ybeF b0629 0.74 -2.9 660,359 - ybeF b0629 0.75 -1.5 660,359 - ybeF b0629 0.75 -2.3 660,364 - ybeF b0629 0.75 +2.5 660,401 - ybeF b0629 0.79 +0.5 660,401 - ybeF b0629 0.79 -0.5 660,403 + ybeF b0629 0.79 -2.5 660,403 + ybeF b0629 0.79 +0.8 660,403 + ybeF b0629 0.79 -2.2 660,403 + ybeF b0629 0.79 -1.2 660,411 - ybeF b0629 0.80 +0.7 660,417 - ybeF b0629 0.81 +2.5 660,420 + ybeF b0629 0.81 +0.3 660,432 - ybeF b0629 0.82 +2.5 660,439 + ybeF b0629 0.83 -0.1 660,476 + ybeF b0629 0.87 -0.7 660,476 + ybeF b0629 0.87 +0.3 660,476 + ybeF b0629 0.87 -1.0 660,476 + ybeF b0629 0.87 -0.9 660,479 + ybeF b0629 0.87 +0.0 660,479 + ybeF b0629 0.87 -0.4 660,505 + ybeF b0629 0.90 +2.9 660,505 + ybeF b0629 0.90 +0.5 660,506 + -0.2 660,527 + -0.5 660,543 + -1.2 660,543 + +0.4 660,552 + +0.4 660,596 + +0.3 660,730 - +2.5 660,850 - +2.5 660,850 - +2.5 661,084 - lipB b0630 0.35 +0.9 661,146 + lipB b0630 0.45 -0.1 661,264 + lipB b0630 0.63 -2.7 661,509 + -0.4 661,519 - +0.8
Or see this region's nucleotide sequence