Experiment: 3hr_MSC_rep2_lowinoculum
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yafL and yafM are separated by 175 nucleotides yafM and lfhA are separated by 223 nucleotides lfhA and mbhA are separated by 1 nucleotides mbhA and dinB are separated by 70 nucleotides
b0227: yafL - predicted lipoprotein and C40 family peptidase (NCBI), at 246,712 to 247,461
yafL
b0228: yafM - hypothetical protein (NCBI), at 247,637 to 248,134
yafM
b0229: lfhA - flagellar biosynthesis (VIMSS), at 248,358 to 250,070
lfhA
b0230: mbhA - putative motility protein (VIMSS), at 250,072 to 250,827
mbhA
b0231: dinB - DNA polymerase IV (NCBI), at 250,898 to 251,953
dinB
Position (kb) 248
249
250
251 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 247.409 kb on - strand at 247.493 kb on + strand at 247.502 kb on - strand at 247.729 kb on + strand, within yafM at 247.786 kb on - strand, within yafM at 247.786 kb on - strand, within yafM at 247.797 kb on - strand, within yafM at 247.824 kb on - strand, within yafM at 247.831 kb on - strand, within yafM at 248.035 kb on + strand, within yafM at 248.067 kb on + strand, within yafM at 248.185 kb on - strand at 248.185 kb on - strand at 248.336 kb on + strand at 248.337 kb on - strand at 248.479 kb on + strand at 248.479 kb on + strand at 248.479 kb on - strand at 248.500 kb on + strand at 248.539 kb on - strand, within lfhA at 248.579 kb on + strand, within lfhA at 248.579 kb on + strand, within lfhA at 248.594 kb on + strand, within lfhA at 248.609 kb on + strand, within lfhA at 248.666 kb on - strand, within lfhA at 248.911 kb on + strand, within lfhA at 248.977 kb on - strand, within lfhA at 249.077 kb on + strand, within lfhA at 249.103 kb on - strand, within lfhA at 249.388 kb on - strand, within lfhA at 249.432 kb on + strand, within lfhA at 249.572 kb on - strand, within lfhA at 249.572 kb on - strand, within lfhA at 249.594 kb on - strand, within lfhA at 249.687 kb on - strand, within lfhA at 249.744 kb on + strand, within lfhA at 250.046 kb on + strand at 250.049 kb on + strand at 250.049 kb on + strand at 250.295 kb on - strand, within mbhA at 250.295 kb on - strand, within mbhA at 250.355 kb on + strand, within mbhA at 250.374 kb on + strand, within mbhA at 250.389 kb on + strand, within mbhA at 250.426 kb on + strand, within mbhA at 250.442 kb on - strand, within mbhA at 250.473 kb on - strand, within mbhA at 250.501 kb on + strand, within mbhA at 250.559 kb on - strand, within mbhA at 250.594 kb on - strand, within mbhA at 250.697 kb on + strand, within mbhA at 250.718 kb on - strand, within mbhA at 250.718 kb on - strand, within mbhA at 250.729 kb on + strand, within mbhA at 250.959 kb on + strand at 250.988 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 3hr_MSC_rep2_lowinoculum remove 247,409 - -0.3 247,493 + +0.6 247,502 - +1.1 247,729 + yafM b0228 0.18 +0.1 247,786 - yafM b0228 0.30 -0.4 247,786 - yafM b0228 0.30 +0.4 247,797 - yafM b0228 0.32 -1.2 247,824 - yafM b0228 0.38 +0.7 247,831 - yafM b0228 0.39 -1.5 248,035 + yafM b0228 0.80 +0.0 248,067 + yafM b0228 0.86 +0.7 248,185 - -0.7 248,185 - +0.1 248,336 + -0.4 248,337 - +0.5 248,479 + +0.0 248,479 + +0.2 248,479 - -3.0 248,500 + -1.2 248,539 - lfhA b0229 0.11 +1.3 248,579 + lfhA b0229 0.13 -0.8 248,579 + lfhA b0229 0.13 +1.3 248,594 + lfhA b0229 0.14 -0.0 248,609 + lfhA b0229 0.15 +0.2 248,666 - lfhA b0229 0.18 +0.4 248,911 + lfhA b0229 0.32 +0.6 248,977 - lfhA b0229 0.36 +0.1 249,077 + lfhA b0229 0.42 +0.6 249,103 - lfhA b0229 0.43 -1.3 249,388 - lfhA b0229 0.60 -0.3 249,432 + lfhA b0229 0.63 +1.1 249,572 - lfhA b0229 0.71 +1.1 249,572 - lfhA b0229 0.71 +0.7 249,594 - lfhA b0229 0.72 +0.3 249,687 - lfhA b0229 0.78 -0.1 249,744 + lfhA b0229 0.81 +0.9 250,046 + -0.1 250,049 + +1.1 250,049 + +0.2 250,295 - mbhA b0230 0.29 -0.4 250,295 - mbhA b0230 0.29 +0.0 250,355 + mbhA b0230 0.37 -1.2 250,374 + mbhA b0230 0.40 +0.0 250,389 + mbhA b0230 0.42 -0.4 250,426 + mbhA b0230 0.47 -2.2 250,442 - mbhA b0230 0.49 -0.3 250,473 - mbhA b0230 0.53 -0.0 250,501 + mbhA b0230 0.57 +0.4 250,559 - mbhA b0230 0.64 -0.4 250,594 - mbhA b0230 0.69 +0.3 250,697 + mbhA b0230 0.83 -0.1 250,718 - mbhA b0230 0.85 +0.8 250,718 - mbhA b0230 0.85 -3.2 250,729 + mbhA b0230 0.87 +0.9 250,959 + +0.3 250,988 - +1.4
Or see this region's nucleotide sequence