Strain Fitness in Escherichia coli BW25113 around b3960

Experiment: 22hr_MSC_rep2_highinoculum

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntargC and argB are separated by 7 nucleotidesargB and argH are separated by 60 nucleotidesargH and oxyS are separated by 61 nucleotidesoxyS and oxyR are separated by 95 nucleotides b3958: argC - N-acetyl-gamma-glutamyl-phosphate reductase (NCBI), at 4,153,024 to 4,154,028 argC b3959: argB - acetylglutamate kinase (RefSeq), at 4,154,036 to 4,154,812 argB b3960: argH - argininosuccinate lyase (NCBI), at 4,154,873 to 4,156,246 argH b4458: oxyS - global regulatory RNA (NCBI), at 4,156,308 to 4,156,417 oxyS b3961: oxyR - DNA-binding transcriptional dual regulator (NCBI), at 4,156,513 to 4,157,430 oxyR Position (kb) 4154 4155 4156 4157Strain fitness (log2 ratio) -2 -1 0 1 2at 4153.917 kb on + strand, within argCat 4153.917 kb on + strand, within argCat 4153.939 kb on + strandat 4153.939 kb on + strandat 4153.972 kb on + strandat 4154.018 kb on + strandat 4154.018 kb on + strandat 4154.023 kb on + strandat 4154.023 kb on + strandat 4154.023 kb on + strandat 4154.074 kb on + strandat 4154.079 kb on + strandat 4154.087 kb on - strandat 4154.090 kb on - strandat 4154.098 kb on - strandat 4154.121 kb on + strand, within argBat 4154.144 kb on + strand, within argBat 4154.153 kb on + strand, within argBat 4154.249 kb on + strand, within argBat 4154.249 kb on + strand, within argBat 4154.353 kb on + strand, within argBat 4154.353 kb on + strand, within argBat 4154.389 kb on + strand, within argBat 4154.394 kb on - strand, within argBat 4154.403 kb on + strand, within argBat 4154.421 kb on + strand, within argBat 4154.421 kb on + strand, within argBat 4154.472 kb on + strand, within argBat 4154.487 kb on - strand, within argBat 4154.497 kb on - strand, within argBat 4154.509 kb on + strand, within argBat 4154.517 kb on - strand, within argBat 4154.549 kb on - strand, within argBat 4154.659 kb on + strand, within argBat 4154.667 kb on - strand, within argBat 4154.667 kb on - strand, within argBat 4154.671 kb on - strand, within argBat 4154.683 kb on - strand, within argBat 4154.683 kb on - strand, within argBat 4154.696 kb on + strand, within argBat 4154.701 kb on + strand, within argBat 4154.707 kb on + strand, within argBat 4154.729 kb on + strand, within argBat 4154.729 kb on + strand, within argBat 4154.750 kb on + strandat 4154.750 kb on + strandat 4154.761 kb on + strandat 4154.761 kb on + strandat 4154.763 kb on + strandat 4154.763 kb on + strandat 4154.771 kb on - strandat 4154.773 kb on + strandat 4154.878 kb on - strandat 4154.900 kb on - strandat 4154.901 kb on + strandat 4154.968 kb on + strandat 4155.017 kb on + strand, within argHat 4155.019 kb on - strand, within argHat 4155.128 kb on + strand, within argHat 4155.128 kb on + strand, within argHat 4155.156 kb on - strand, within argHat 4155.178 kb on + strand, within argHat 4155.178 kb on + strand, within argHat 4155.193 kb on - strand, within argHat 4155.193 kb on - strand, within argHat 4155.229 kb on - strand, within argHat 4155.237 kb on - strand, within argHat 4155.237 kb on - strand, within argHat 4155.237 kb on - strand, within argHat 4155.336 kb on - strand, within argHat 4155.338 kb on + strand, within argHat 4155.366 kb on - strand, within argHat 4155.366 kb on - strand, within argHat 4155.373 kb on + strand, within argHat 4155.381 kb on - strand, within argHat 4155.385 kb on - strand, within argHat 4155.436 kb on - strand, within argHat 4155.437 kb on + strand, within argHat 4155.437 kb on + strand, within argHat 4155.619 kb on - strand, within argHat 4155.699 kb on - strand, within argHat 4155.699 kb on - strand, within argHat 4155.713 kb on - strand, within argHat 4155.713 kb on - strand, within argHat 4155.767 kb on + strand, within argHat 4155.767 kb on + strand, within argHat 4155.770 kb on - strand, within argHat 4155.770 kb on - strand, within argHat 4155.865 kb on + strand, within argHat 4155.875 kb on + strand, within argHat 4155.876 kb on + strand, within argHat 4155.883 kb on - strand, within argHat 4155.885 kb on + strand, within argHat 4155.885 kb on + strand, within argHat 4155.958 kb on + strand, within argHat 4155.961 kb on - strand, within argHat 4156.012 kb on + strand, within argHat 4156.024 kb on + strand, within argHat 4156.024 kb on + strand, within argHat 4156.034 kb on - strand, within argHat 4156.046 kb on + strand, within argHat 4156.054 kb on - strand, within argHat 4156.117 kb on + strandat 4156.156 kb on + strandat 4156.156 kb on + strandat 4156.196 kb on + strandat 4156.196 kb on + strandat 4156.215 kb on - strandat 4156.235 kb on + strandat 4156.239 kb on - strandat 4156.322 kb on - strand, within oxySat 4156.322 kb on - strand, within oxySat 4156.476 kb on - strandat 4156.476 kb on - strandat 4156.476 kb on - strandat 4156.517 kb on - strandat 4156.555 kb on - strandat 4156.633 kb on - strand, within oxyRat 4156.633 kb on - strand, within oxyRat 4156.667 kb on + strand, within oxyRat 4156.703 kb on + strand, within oxyRat 4156.703 kb on + strand, within oxyRat 4156.750 kb on - strand, within oxyRat 4156.757 kb on + strand, within oxyRat 4156.775 kb on - strand, within oxyRat 4156.841 kb on + strand, within oxyRat 4156.841 kb on + strand, within oxyRat 4156.886 kb on + strand, within oxyRat 4156.886 kb on + strand, within oxyRat 4156.899 kb on - strand, within oxyRat 4156.926 kb on - strand, within oxyRat 4156.926 kb on - strand, within oxyRat 4157.000 kb on + strand, within oxyRat 4157.001 kb on + strand, within oxyRat 4157.021 kb on + strand, within oxyRat 4157.031 kb on + strand, within oxyRat 4157.050 kb on + strand, within oxyRat 4157.156 kb on + strand, within oxyRat 4157.166 kb on + strand, within oxyRat 4157.166 kb on + strand, within oxyRat 4157.180 kb on + strand, within oxyRat 4157.204 kb on - strand, within oxyRat 4157.213 kb on + strand, within oxyRat 4157.213 kb on + strand, within oxyRat 4157.230 kb on - strand, within oxyR

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Per-strain Table

Position Strand Gene LocusTag Fraction 22hr_MSC_rep2_highinoculum
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4,153,917 + argC b3958 0.89 +0.4
4,153,917 + argC b3958 0.89 -1.0
4,153,939 + +0.1
4,153,939 + +0.3
4,153,972 + -0.0
4,154,018 + +0.0
4,154,018 + -0.8
4,154,023 + -0.4
4,154,023 + +0.5
4,154,023 + +0.2
4,154,074 + +0.1
4,154,079 + +0.4
4,154,087 - +0.0
4,154,090 - +0.4
4,154,098 - +1.1
4,154,121 + argB b3959 0.11 -0.5
4,154,144 + argB b3959 0.14 -0.1
4,154,153 + argB b3959 0.15 -0.7
4,154,249 + argB b3959 0.27 +0.8
4,154,249 + argB b3959 0.27 +0.8
4,154,353 + argB b3959 0.41 -0.4
4,154,353 + argB b3959 0.41 -0.3
4,154,389 + argB b3959 0.45 -1.5
4,154,394 - argB b3959 0.46 +0.1
4,154,403 + argB b3959 0.47 -0.1
4,154,421 + argB b3959 0.50 +0.1
4,154,421 + argB b3959 0.50 +0.6
4,154,472 + argB b3959 0.56 +0.2
4,154,487 - argB b3959 0.58 -0.4
4,154,497 - argB b3959 0.59 -1.6
4,154,509 + argB b3959 0.61 +0.2
4,154,517 - argB b3959 0.62 +0.0
4,154,549 - argB b3959 0.66 -0.5
4,154,659 + argB b3959 0.80 +0.5
4,154,667 - argB b3959 0.81 -0.2
4,154,667 - argB b3959 0.81 -0.1
4,154,671 - argB b3959 0.82 -0.1
4,154,683 - argB b3959 0.83 +0.5
4,154,683 - argB b3959 0.83 +0.2
4,154,696 + argB b3959 0.85 -1.1
4,154,701 + argB b3959 0.86 +0.4
4,154,707 + argB b3959 0.86 -0.3
4,154,729 + argB b3959 0.89 -0.5
4,154,729 + argB b3959 0.89 -0.2
4,154,750 + +0.2
4,154,750 + -0.3
4,154,761 + +0.9
4,154,761 + +0.2
4,154,763 + -0.3
4,154,763 + +0.3
4,154,771 - +0.2
4,154,773 + -1.1
4,154,878 - +0.1
4,154,900 - +2.1
4,154,901 + -0.6
4,154,968 + +0.2
4,155,017 + argH b3960 0.10 -0.4
4,155,019 - argH b3960 0.11 +0.5
4,155,128 + argH b3960 0.19 -0.6
4,155,128 + argH b3960 0.19 -0.7
4,155,156 - argH b3960 0.21 -0.9
4,155,178 + argH b3960 0.22 -0.3
4,155,178 + argH b3960 0.22 -0.5
4,155,193 - argH b3960 0.23 -1.2
4,155,193 - argH b3960 0.23 +0.3
4,155,229 - argH b3960 0.26 -0.1
4,155,237 - argH b3960 0.26 +0.7
4,155,237 - argH b3960 0.26 -1.1
4,155,237 - argH b3960 0.26 +0.1
4,155,336 - argH b3960 0.34 +0.5
4,155,338 + argH b3960 0.34 +0.1
4,155,366 - argH b3960 0.36 -0.6
4,155,366 - argH b3960 0.36 -0.4
4,155,373 + argH b3960 0.36 +0.5
4,155,381 - argH b3960 0.37 -0.3
4,155,385 - argH b3960 0.37 -0.0
4,155,436 - argH b3960 0.41 +0.1
4,155,437 + argH b3960 0.41 -0.9
4,155,437 + argH b3960 0.41 -0.1
4,155,619 - argH b3960 0.54 -1.3
4,155,699 - argH b3960 0.60 +0.2
4,155,699 - argH b3960 0.60 -0.7
4,155,713 - argH b3960 0.61 -0.0
4,155,713 - argH b3960 0.61 +0.2
4,155,767 + argH b3960 0.65 -0.5
4,155,767 + argH b3960 0.65 +0.6
4,155,770 - argH b3960 0.65 +0.2
4,155,770 - argH b3960 0.65 +0.1
4,155,865 + argH b3960 0.72 -0.2
4,155,875 + argH b3960 0.73 +0.0
4,155,876 + argH b3960 0.73 +0.7
4,155,883 - argH b3960 0.74 -0.1
4,155,885 + argH b3960 0.74 -0.1
4,155,885 + argH b3960 0.74 +0.2
4,155,958 + argH b3960 0.79 +0.5
4,155,961 - argH b3960 0.79 -0.0
4,156,012 + argH b3960 0.83 +0.2
4,156,024 + argH b3960 0.84 +0.7
4,156,024 + argH b3960 0.84 +1.4
4,156,034 - argH b3960 0.84 +0.2
4,156,046 + argH b3960 0.85 +0.8
4,156,054 - argH b3960 0.86 +0.1
4,156,117 + +0.3
4,156,156 + -0.8
4,156,156 + +0.0
4,156,196 + -1.1
4,156,196 + -0.1
4,156,215 - +0.1
4,156,235 + -0.3
4,156,239 - +0.0
4,156,322 - oxyS b4458 0.13 +0.1
4,156,322 - oxyS b4458 0.13 -0.1
4,156,476 - +0.3
4,156,476 - -0.2
4,156,476 - -2.0
4,156,517 - -0.8
4,156,555 - -0.2
4,156,633 - oxyR b3961 0.13 -0.6
4,156,633 - oxyR b3961 0.13 -0.8
4,156,667 + oxyR b3961 0.17 -0.9
4,156,703 + oxyR b3961 0.21 -0.6
4,156,703 + oxyR b3961 0.21 -1.3
4,156,750 - oxyR b3961 0.26 +0.1
4,156,757 + oxyR b3961 0.27 -2.7
4,156,775 - oxyR b3961 0.29 -0.3
4,156,841 + oxyR b3961 0.36 -1.8
4,156,841 + oxyR b3961 0.36 -0.5
4,156,886 + oxyR b3961 0.41 -0.5
4,156,886 + oxyR b3961 0.41 -0.3
4,156,899 - oxyR b3961 0.42 -0.6
4,156,926 - oxyR b3961 0.45 -1.1
4,156,926 - oxyR b3961 0.45 -1.4
4,157,000 + oxyR b3961 0.53 -2.1
4,157,001 + oxyR b3961 0.53 -0.4
4,157,021 + oxyR b3961 0.55 -1.3
4,157,031 + oxyR b3961 0.56 -0.1
4,157,050 + oxyR b3961 0.58 -0.9
4,157,156 + oxyR b3961 0.70 -1.1
4,157,166 + oxyR b3961 0.71 -2.6
4,157,166 + oxyR b3961 0.71 -1.5
4,157,180 + oxyR b3961 0.73 -1.2
4,157,204 - oxyR b3961 0.75 -0.3
4,157,213 + oxyR b3961 0.76 +0.1
4,157,213 + oxyR b3961 0.76 -0.9
4,157,230 - oxyR b3961 0.78 -1.0

Or see this region's nucleotide sequence