Experiment: 22hr_MSC_rep1_highinoculum
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yhgA and bioH are separated by 28 nucleotides bioH and yhgH are separated by 37 nucleotides yhgH and gntY are separated by 58 nucleotides gntY and gntT are separated by 359 nucleotides
b3411: yhgA - predicted transposase (NCBI), at 3,541,189 to 3,542,067
yhgA
b3412: bioH - carboxylesterase of pimeloyl-CoA synthesis (NCBI), at 3,542,096 to 3,542,866
bioH
b3413: yhgH - orf, hypothetical protein (VIMSS), at 3,542,904 to 3,543,587
yhgH
b3414: gntY - predicted gluconate transport associated protein (NCBI), at 3,543,646 to 3,544,221
gntY
b3415: gntT - gluconate transporter, high-affinity GNT I system (NCBI), at 3,544,581 to 3,545,897
gntT
Position (kb)
3542
3543
3544 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3541.913 kb on + strand, within yhgA at 3541.963 kb on + strand, within yhgA at 3541.968 kb on + strand, within yhgA at 3541.986 kb on - strand at 3541.986 kb on - strand at 3542.095 kb on + strand at 3542.103 kb on - strand at 3542.109 kb on - strand at 3542.115 kb on - strand at 3542.182 kb on - strand, within bioH at 3542.182 kb on - strand, within bioH at 3542.236 kb on + strand, within bioH at 3542.244 kb on - strand, within bioH at 3542.244 kb on - strand, within bioH at 3542.244 kb on - strand, within bioH at 3542.244 kb on - strand, within bioH at 3542.361 kb on + strand, within bioH at 3542.361 kb on + strand, within bioH at 3542.366 kb on + strand, within bioH at 3542.405 kb on - strand, within bioH at 3542.441 kb on - strand, within bioH at 3542.576 kb on + strand, within bioH at 3542.576 kb on + strand, within bioH at 3542.580 kb on + strand, within bioH at 3542.584 kb on + strand, within bioH at 3542.593 kb on - strand, within bioH at 3542.692 kb on + strand, within bioH at 3542.741 kb on - strand, within bioH at 3542.741 kb on - strand, within bioH at 3542.767 kb on + strand, within bioH at 3542.767 kb on + strand, within bioH at 3542.775 kb on - strand, within bioH at 3542.775 kb on - strand, within bioH at 3542.785 kb on - strand, within bioH at 3542.797 kb on + strand at 3542.797 kb on + strand at 3542.800 kb on - strand at 3542.805 kb on - strand at 3542.805 kb on - strand at 3542.805 kb on - strand at 3542.812 kb on - strand at 3542.826 kb on + strand at 3542.830 kb on + strand at 3542.830 kb on + strand at 3542.893 kb on + strand at 3542.903 kb on + strand at 3542.903 kb on + strand at 3542.953 kb on - strand at 3542.953 kb on - strand at 3542.974 kb on - strand, within yhgH at 3542.974 kb on - strand, within yhgH at 3543.006 kb on + strand, within yhgH at 3543.028 kb on - strand, within yhgH at 3543.093 kb on - strand, within yhgH at 3543.102 kb on + strand, within yhgH at 3543.102 kb on + strand, within yhgH at 3543.130 kb on + strand, within yhgH at 3543.140 kb on - strand, within yhgH at 3543.185 kb on - strand, within yhgH at 3543.194 kb on - strand, within yhgH at 3543.194 kb on - strand, within yhgH at 3543.214 kb on + strand, within yhgH at 3543.218 kb on + strand, within yhgH at 3543.300 kb on - strand, within yhgH at 3543.362 kb on + strand, within yhgH at 3543.377 kb on + strand, within yhgH at 3543.379 kb on - strand, within yhgH at 3543.379 kb on - strand, within yhgH at 3543.385 kb on - strand, within yhgH at 3543.411 kb on - strand, within yhgH at 3543.411 kb on - strand, within yhgH at 3543.450 kb on - strand, within yhgH at 3543.472 kb on + strand, within yhgH at 3543.477 kb on + strand, within yhgH at 3543.552 kb on - strand at 3543.555 kb on + strand at 3543.580 kb on - strand at 3543.580 kb on - strand at 3543.595 kb on + strand at 3543.632 kb on + strand at 3543.706 kb on + strand, within gntY at 3543.706 kb on + strand, within gntY at 3543.727 kb on + strand, within gntY at 3543.737 kb on + strand, within gntY at 3543.737 kb on + strand, within gntY at 3543.737 kb on + strand, within gntY at 3543.790 kb on + strand, within gntY at 3543.796 kb on + strand, within gntY at 3543.808 kb on + strand, within gntY at 3543.836 kb on + strand, within gntY at 3543.836 kb on + strand, within gntY at 3543.849 kb on + strand, within gntY at 3543.849 kb on + strand, within gntY at 3543.860 kb on + strand, within gntY at 3543.953 kb on + strand, within gntY at 3543.964 kb on + strand, within gntY at 3543.966 kb on + strand, within gntY at 3543.966 kb on + strand, within gntY at 3543.988 kb on - strand, within gntY at 3543.991 kb on - strand, within gntY at 3544.004 kb on + strand, within gntY at 3544.026 kb on - strand, within gntY at 3544.095 kb on + strand, within gntY at 3544.181 kb on + strand at 3544.232 kb on + strand at 3544.232 kb on + strand at 3544.232 kb on + strand at 3544.240 kb on - strand at 3544.240 kb on - strand at 3544.286 kb on - strand at 3544.286 kb on - strand at 3544.288 kb on - strand at 3544.305 kb on + strand at 3544.320 kb on - strand at 3544.322 kb on - strand at 3544.329 kb on - strand at 3544.329 kb on - strand at 3544.391 kb on + strand at 3544.404 kb on + strand at 3544.404 kb on + strand at 3544.456 kb on - strand at 3544.457 kb on + strand at 3544.457 kb on + strand at 3544.493 kb on + strand at 3544.507 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 22hr_MSC_rep1_highinoculum remove 3,541,913 + yhgA b3411 0.82 +0.2 3,541,963 + yhgA b3411 0.88 +0.3 3,541,968 + yhgA b3411 0.89 -0.3 3,541,986 - +2.2 3,541,986 - -0.1 3,542,095 + +1.3 3,542,103 - -0.2 3,542,109 - +0.9 3,542,115 - +0.1 3,542,182 - bioH b3412 0.11 +0.4 3,542,182 - bioH b3412 0.11 +0.9 3,542,236 + bioH b3412 0.18 +0.0 3,542,244 - bioH b3412 0.19 +0.6 3,542,244 - bioH b3412 0.19 -0.7 3,542,244 - bioH b3412 0.19 -0.6 3,542,244 - bioH b3412 0.19 +0.4 3,542,361 + bioH b3412 0.34 -0.4 3,542,361 + bioH b3412 0.34 -0.0 3,542,366 + bioH b3412 0.35 -0.7 3,542,405 - bioH b3412 0.40 -0.0 3,542,441 - bioH b3412 0.45 +2.4 3,542,576 + bioH b3412 0.62 +0.2 3,542,576 + bioH b3412 0.62 +1.2 3,542,580 + bioH b3412 0.63 -1.0 3,542,584 + bioH b3412 0.63 -0.1 3,542,593 - bioH b3412 0.64 -1.0 3,542,692 + bioH b3412 0.77 +0.5 3,542,741 - bioH b3412 0.84 +0.6 3,542,741 - bioH b3412 0.84 -0.8 3,542,767 + bioH b3412 0.87 +1.4 3,542,767 + bioH b3412 0.87 +0.6 3,542,775 - bioH b3412 0.88 +0.6 3,542,775 - bioH b3412 0.88 -0.7 3,542,785 - bioH b3412 0.89 -1.3 3,542,797 + +0.6 3,542,797 + -1.1 3,542,800 - +0.5 3,542,805 - -0.3 3,542,805 - -2.6 3,542,805 - -0.5 3,542,812 - +0.7 3,542,826 + -1.3 3,542,830 + +0.2 3,542,830 + +0.3 3,542,893 + +0.9 3,542,903 + +0.1 3,542,903 + +0.1 3,542,953 - -0.7 3,542,953 - -0.0 3,542,974 - yhgH b3413 0.10 -1.1 3,542,974 - yhgH b3413 0.10 -0.2 3,543,006 + yhgH b3413 0.15 -4.0 3,543,028 - yhgH b3413 0.18 -0.1 3,543,093 - yhgH b3413 0.28 +0.1 3,543,102 + yhgH b3413 0.29 -1.8 3,543,102 + yhgH b3413 0.29 -1.0 3,543,130 + yhgH b3413 0.33 +0.5 3,543,140 - yhgH b3413 0.35 +0.2 3,543,185 - yhgH b3413 0.41 -0.9 3,543,194 - yhgH b3413 0.42 -0.5 3,543,194 - yhgH b3413 0.42 +1.2 3,543,214 + yhgH b3413 0.45 -2.6 3,543,218 + yhgH b3413 0.46 -0.9 3,543,300 - yhgH b3413 0.58 +0.2 3,543,362 + yhgH b3413 0.67 +0.8 3,543,377 + yhgH b3413 0.69 -0.8 3,543,379 - yhgH b3413 0.69 +0.2 3,543,379 - yhgH b3413 0.69 -0.4 3,543,385 - yhgH b3413 0.70 -0.7 3,543,411 - yhgH b3413 0.74 -0.3 3,543,411 - yhgH b3413 0.74 +0.8 3,543,450 - yhgH b3413 0.80 +0.4 3,543,472 + yhgH b3413 0.83 -3.6 3,543,477 + yhgH b3413 0.84 -1.3 3,543,552 - +0.7 3,543,555 + -4.5 3,543,580 - +0.8 3,543,580 - +1.6 3,543,595 + +0.3 3,543,632 + +0.7 3,543,706 + gntY b3414 0.10 -2.6 3,543,706 + gntY b3414 0.10 -2.7 3,543,727 + gntY b3414 0.14 +0.1 3,543,737 + gntY b3414 0.16 +1.2 3,543,737 + gntY b3414 0.16 -1.0 3,543,737 + gntY b3414 0.16 +2.8 3,543,790 + gntY b3414 0.25 -2.7 3,543,796 + gntY b3414 0.26 -1.2 3,543,808 + gntY b3414 0.28 -2.6 3,543,836 + gntY b3414 0.33 -1.8 3,543,836 + gntY b3414 0.33 +1.9 3,543,849 + gntY b3414 0.35 -1.0 3,543,849 + gntY b3414 0.35 -2.1 3,543,860 + gntY b3414 0.37 -0.5 3,543,953 + gntY b3414 0.53 -0.3 3,543,964 + gntY b3414 0.55 -0.0 3,543,966 + gntY b3414 0.56 -0.8 3,543,966 + gntY b3414 0.56 -1.6 3,543,988 - gntY b3414 0.59 -2.1 3,543,991 - gntY b3414 0.60 +0.2 3,544,004 + gntY b3414 0.62 -3.2 3,544,026 - gntY b3414 0.66 +0.2 3,544,095 + gntY b3414 0.78 +1.1 3,544,181 + +0.4 3,544,232 + -0.6 3,544,232 + -0.7 3,544,232 + +0.1 3,544,240 - +0.3 3,544,240 - -1.4 3,544,286 - +0.5 3,544,286 - +0.1 3,544,288 - +0.9 3,544,305 + +0.6 3,544,320 - -1.6 3,544,322 - -0.2 3,544,329 - +0.5 3,544,329 - +0.6 3,544,391 + +0.4 3,544,404 + +0.2 3,544,404 + +1.2 3,544,456 - +1.0 3,544,457 + +0.8 3,544,457 + +0.6 3,544,493 + +0.3 3,544,507 - -0.6
Or see this region's nucleotide sequence