Experiment: 3hr_MSC_rep2_highinoculum
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mazG and chpA are separated by 70 nucleotides chpA and chpR overlap by 1 nucleotides chpR and relA are separated by 77 nucleotides relA and rumA are separated by 47 nucleotides
b2781: mazG - nucleoside triphosphate pyrophosphohydrolase (NCBI), at 2,907,916 to 2,908,707
mazG
b2782: chpA - toxin of the ChpA-ChpR toxin-antitoxin system, endoribonuclease (NCBI), at 2,908,778 to 2,909,113
chpA
b2783: chpR - antitoxin of the ChpA-ChpR toxin-antitoxin system (NCBI), at 2,909,113 to 2,909,361
chpR
b2784: relA - (p)ppGpp synthetase I/GTP pyrophosphokinase (NCBI), at 2,909,439 to 2,911,673
relA
b2785: rumA - 23S rRNA (uracil-5-)-methyltransferase (NCBI), at 2,911,721 to 2,913,022
rumA
Position (kb)
2909
2910
2911
2912 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2908.538 kb on + strand, within mazG at 2908.571 kb on + strand, within mazG at 2908.624 kb on + strand, within mazG at 2908.674 kb on + strand at 2908.674 kb on + strand at 2908.674 kb on + strand at 2908.674 kb on + strand at 2908.682 kb on - strand at 2908.754 kb on - strand at 2908.754 kb on - strand at 2908.764 kb on - strand at 2908.764 kb on - strand at 2908.772 kb on + strand at 2908.772 kb on + strand at 2908.854 kb on + strand, within chpA at 2908.991 kb on - strand, within chpA at 2909.017 kb on - strand, within chpA at 2909.017 kb on - strand, within chpA at 2909.032 kb on + strand, within chpA at 2909.032 kb on + strand, within chpA at 2909.253 kb on + strand, within chpR at 2909.258 kb on + strand, within chpR at 2909.311 kb on + strand, within chpR at 2909.487 kb on + strand at 2909.487 kb on + strand at 2909.490 kb on - strand at 2909.490 kb on - strand at 2909.556 kb on - strand at 2909.669 kb on - strand, within relA at 2909.776 kb on + strand, within relA at 2909.776 kb on + strand, within relA at 2910.051 kb on - strand, within relA at 2910.051 kb on - strand, within relA at 2910.174 kb on + strand, within relA at 2910.174 kb on + strand, within relA at 2910.294 kb on - strand, within relA at 2910.313 kb on + strand, within relA at 2910.476 kb on + strand, within relA at 2910.476 kb on + strand, within relA at 2910.488 kb on - strand, within relA at 2910.567 kb on + strand, within relA at 2910.632 kb on + strand, within relA at 2910.632 kb on + strand, within relA at 2910.685 kb on + strand, within relA at 2910.721 kb on - strand, within relA at 2910.721 kb on - strand, within relA at 2910.721 kb on - strand, within relA at 2910.788 kb on - strand, within relA at 2910.788 kb on - strand, within relA at 2910.788 kb on - strand, within relA at 2910.895 kb on - strand, within relA at 2910.895 kb on - strand, within relA at 2910.898 kb on + strand, within relA at 2910.898 kb on + strand, within relA at 2910.921 kb on + strand, within relA at 2910.921 kb on + strand, within relA at 2910.985 kb on + strand, within relA at 2911.020 kb on - strand, within relA at 2911.134 kb on - strand, within relA at 2911.196 kb on + strand, within relA at 2911.201 kb on + strand, within relA at 2911.201 kb on + strand, within relA at 2911.283 kb on + strand, within relA at 2911.283 kb on + strand, within relA at 2911.283 kb on + strand, within relA at 2911.297 kb on + strand, within relA at 2911.300 kb on - strand, within relA at 2911.301 kb on - strand, within relA at 2911.301 kb on - strand, within relA at 2911.331 kb on + strand, within relA at 2911.331 kb on + strand, within relA at 2911.360 kb on - strand, within relA at 2911.456 kb on - strand at 2911.488 kb on - strand at 2911.525 kb on + strand at 2911.525 kb on + strand at 2911.568 kb on - strand at 2911.568 kb on - strand at 2911.572 kb on + strand at 2911.572 kb on + strand at 2911.598 kb on + strand at 2911.690 kb on - strand at 2911.795 kb on - strand at 2911.851 kb on + strand at 2911.851 kb on + strand at 2911.854 kb on + strand, within rumA at 2911.859 kb on + strand, within rumA at 2911.860 kb on - strand, within rumA at 2911.861 kb on - strand, within rumA at 2911.870 kb on + strand, within rumA at 2911.903 kb on + strand, within rumA at 2911.931 kb on - strand, within rumA at 2911.978 kb on - strand, within rumA at 2912.077 kb on + strand, within rumA at 2912.087 kb on + strand, within rumA at 2912.087 kb on + strand, within rumA at 2912.117 kb on - strand, within rumA at 2912.168 kb on + strand, within rumA at 2912.263 kb on + strand, within rumA at 2912.343 kb on - strand, within rumA at 2912.387 kb on + strand, within rumA at 2912.433 kb on + strand, within rumA at 2912.462 kb on + strand, within rumA at 2912.465 kb on - strand, within rumA at 2912.480 kb on + strand, within rumA at 2912.556 kb on + strand, within rumA at 2912.559 kb on + strand, within rumA at 2912.646 kb on + strand, within rumA at 2912.654 kb on - strand, within rumA at 2912.668 kb on + strand, within rumA at 2912.668 kb on + strand, within rumA at 2912.668 kb on + strand, within rumA at 2912.668 kb on + strand, within rumA
Per-strain Table
Position Strand Gene LocusTag Fraction 3hr_MSC_rep2_highinoculum remove 2,908,538 + mazG b2781 0.79 -0.8 2,908,571 + mazG b2781 0.83 -0.3 2,908,624 + mazG b2781 0.89 -1.1 2,908,674 + +0.2 2,908,674 + -0.5 2,908,674 + -0.4 2,908,674 + +0.5 2,908,682 - +0.2 2,908,754 - -0.3 2,908,754 - -0.4 2,908,764 - +0.2 2,908,764 - +0.0 2,908,772 + -0.4 2,908,772 + -0.9 2,908,854 + chpA b2782 0.23 +0.0 2,908,991 - chpA b2782 0.63 -0.9 2,909,017 - chpA b2782 0.71 -0.7 2,909,017 - chpA b2782 0.71 -0.2 2,909,032 + chpA b2782 0.76 -0.3 2,909,032 + chpA b2782 0.76 +0.2 2,909,253 + chpR b2783 0.56 -0.4 2,909,258 + chpR b2783 0.58 -0.2 2,909,311 + chpR b2783 0.80 +0.1 2,909,487 + -0.7 2,909,487 + -1.3 2,909,490 - -0.2 2,909,490 - -1.1 2,909,556 - -2.5 2,909,669 - relA b2784 0.10 -1.4 2,909,776 + relA b2784 0.15 -0.9 2,909,776 + relA b2784 0.15 -3.5 2,910,051 - relA b2784 0.27 -1.1 2,910,051 - relA b2784 0.27 -1.5 2,910,174 + relA b2784 0.33 -2.9 2,910,174 + relA b2784 0.33 -2.2 2,910,294 - relA b2784 0.38 -1.4 2,910,313 + relA b2784 0.39 -0.6 2,910,476 + relA b2784 0.46 -0.9 2,910,476 + relA b2784 0.46 -3.9 2,910,488 - relA b2784 0.47 -2.2 2,910,567 + relA b2784 0.50 -2.2 2,910,632 + relA b2784 0.53 +0.6 2,910,632 + relA b2784 0.53 -0.7 2,910,685 + relA b2784 0.56 -2.3 2,910,721 - relA b2784 0.57 -1.7 2,910,721 - relA b2784 0.57 -3.2 2,910,721 - relA b2784 0.57 -2.7 2,910,788 - relA b2784 0.60 -2.5 2,910,788 - relA b2784 0.60 -1.9 2,910,788 - relA b2784 0.60 -1.9 2,910,895 - relA b2784 0.65 -2.4 2,910,895 - relA b2784 0.65 -2.2 2,910,898 + relA b2784 0.65 -1.5 2,910,898 + relA b2784 0.65 -2.3 2,910,921 + relA b2784 0.66 -2.5 2,910,921 + relA b2784 0.66 -0.7 2,910,985 + relA b2784 0.69 -1.6 2,911,020 - relA b2784 0.71 -2.5 2,911,134 - relA b2784 0.76 -1.4 2,911,196 + relA b2784 0.79 -4.4 2,911,201 + relA b2784 0.79 -1.6 2,911,201 + relA b2784 0.79 -3.0 2,911,283 + relA b2784 0.83 +0.5 2,911,283 + relA b2784 0.83 -2.5 2,911,283 + relA b2784 0.83 -2.2 2,911,297 + relA b2784 0.83 -1.8 2,911,300 - relA b2784 0.83 -1.9 2,911,301 - relA b2784 0.83 -0.2 2,911,301 - relA b2784 0.83 -1.1 2,911,331 + relA b2784 0.85 -2.0 2,911,331 + relA b2784 0.85 -1.2 2,911,360 - relA b2784 0.86 -0.9 2,911,456 - -2.7 2,911,488 - -1.2 2,911,525 + -1.9 2,911,525 + -4.2 2,911,568 - -2.0 2,911,568 - -1.4 2,911,572 + -0.8 2,911,572 + -2.2 2,911,598 + -0.7 2,911,690 - +0.2 2,911,795 - +0.5 2,911,851 + -2.0 2,911,851 + -1.7 2,911,854 + rumA b2785 0.10 -0.2 2,911,859 + rumA b2785 0.11 -1.9 2,911,860 - rumA b2785 0.11 -0.5 2,911,861 - rumA b2785 0.11 -0.1 2,911,870 + rumA b2785 0.11 -0.1 2,911,903 + rumA b2785 0.14 -0.8 2,911,931 - rumA b2785 0.16 +0.8 2,911,978 - rumA b2785 0.20 -0.2 2,912,077 + rumA b2785 0.27 -0.1 2,912,087 + rumA b2785 0.28 -0.3 2,912,087 + rumA b2785 0.28 -0.5 2,912,117 - rumA b2785 0.30 +0.2 2,912,168 + rumA b2785 0.34 -0.5 2,912,263 + rumA b2785 0.42 -0.2 2,912,343 - rumA b2785 0.48 +0.1 2,912,387 + rumA b2785 0.51 -0.1 2,912,433 + rumA b2785 0.55 -0.4 2,912,462 + rumA b2785 0.57 +0.0 2,912,465 - rumA b2785 0.57 -0.0 2,912,480 + rumA b2785 0.58 -0.2 2,912,556 + rumA b2785 0.64 -0.0 2,912,559 + rumA b2785 0.64 -0.7 2,912,646 + rumA b2785 0.71 -0.8 2,912,654 - rumA b2785 0.72 +0.0 2,912,668 + rumA b2785 0.73 +0.0 2,912,668 + rumA b2785 0.73 -0.2 2,912,668 + rumA b2785 0.73 +0.3 2,912,668 + rumA b2785 0.73 +0.1
Or see this region's nucleotide sequence