Strain Fitness in Escherichia coli BW25113 around b0592

Experiment: 3hr_MSC_rep2_highinoculum

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntybdA and fepB are separated by 3 nucleotidesfepB and entC are separated by 374 nucleotides b0591: ybdA - predicted transporter (NCBI), at 621,523 to 622,773 ybdA b0592: fepB - iron-enterobactin transporter subunit (NCBI), at 622,777 to 623,733 fepB b0593: entC - isochorismate synthase (NCBI), at 624,108 to 625,283 entC Position (kb) 622 623 624Strain fitness (log2 ratio) -2 -1 0 1at 621.819 kb on + strand, within ybdAat 621.846 kb on + strand, within ybdAat 621.846 kb on + strand, within ybdAat 621.966 kb on + strand, within ybdAat 622.236 kb on + strand, within ybdAat 622.246 kb on + strand, within ybdAat 622.255 kb on - strand, within ybdAat 622.255 kb on - strand, within ybdAat 622.365 kb on + strand, within ybdAat 622.365 kb on + strand, within ybdAat 622.409 kb on - strand, within ybdAat 622.434 kb on + strand, within ybdAat 622.441 kb on - strand, within ybdAat 622.441 kb on - strand, within ybdAat 622.472 kb on + strand, within ybdAat 622.472 kb on + strand, within ybdAat 622.491 kb on + strand, within ybdAat 622.596 kb on + strand, within ybdAat 622.596 kb on + strand, within ybdAat 622.611 kb on - strand, within ybdAat 622.611 kb on - strand, within ybdAat 622.615 kb on + strand, within ybdAat 622.615 kb on + strand, within ybdAat 622.801 kb on - strandat 622.819 kb on + strandat 622.819 kb on + strandat 622.847 kb on - strandat 622.875 kb on + strand, within fepBat 622.875 kb on + strand, within fepBat 622.899 kb on + strand, within fepBat 622.917 kb on + strand, within fepBat 622.923 kb on + strand, within fepBat 622.958 kb on + strand, within fepBat 623.019 kb on + strand, within fepBat 623.019 kb on + strand, within fepBat 623.028 kb on - strand, within fepBat 623.028 kb on - strand, within fepBat 623.032 kb on + strand, within fepBat 623.119 kb on + strand, within fepBat 623.140 kb on - strand, within fepBat 623.148 kb on - strand, within fepBat 623.148 kb on - strand, within fepBat 623.199 kb on - strand, within fepBat 623.253 kb on - strand, within fepBat 623.341 kb on - strand, within fepBat 623.405 kb on - strand, within fepBat 623.405 kb on - strand, within fepBat 623.426 kb on + strand, within fepBat 623.426 kb on + strand, within fepBat 623.458 kb on - strand, within fepBat 623.458 kb on - strand, within fepBat 623.467 kb on - strand, within fepBat 623.467 kb on - strand, within fepBat 623.470 kb on - strand, within fepBat 623.470 kb on - strand, within fepBat 623.563 kb on + strand, within fepBat 623.576 kb on - strand, within fepBat 623.576 kb on - strand, within fepBat 623.591 kb on - strand, within fepBat 623.591 kb on - strand, within fepBat 623.610 kb on + strand, within fepBat 623.672 kb on + strandat 623.708 kb on + strandat 623.806 kb on + strandat 623.822 kb on + strandat 623.822 kb on + strandat 623.830 kb on - strandat 623.832 kb on - strandat 623.841 kb on + strandat 623.857 kb on + strandat 623.865 kb on + strandat 623.869 kb on + strandat 623.869 kb on + strandat 623.879 kb on - strandat 623.907 kb on - strandat 623.907 kb on - strandat 624.008 kb on + strandat 624.130 kb on - strandat 624.130 kb on - strandat 624.138 kb on - strandat 624.138 kb on - strandat 624.259 kb on + strand, within entCat 624.428 kb on - strand, within entCat 624.428 kb on - strand, within entCat 624.490 kb on + strand, within entCat 624.547 kb on - strand, within entCat 624.580 kb on - strand, within entCat 624.691 kb on + strand, within entCat 624.691 kb on + strand, within entCat 624.699 kb on - strand, within entCat 624.699 kb on - strand, within entCat 624.699 kb on - strand, within entC

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Per-strain Table

Position Strand Gene LocusTag Fraction 3hr_MSC_rep2_highinoculum
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621,819 + ybdA b0591 0.24 -0.2
621,846 + ybdA b0591 0.26 -0.4
621,846 + ybdA b0591 0.26 +0.0
621,966 + ybdA b0591 0.35 -0.1
622,236 + ybdA b0591 0.57 -0.0
622,246 + ybdA b0591 0.58 -0.3
622,255 - ybdA b0591 0.59 +0.0
622,255 - ybdA b0591 0.59 +0.3
622,365 + ybdA b0591 0.67 -0.3
622,365 + ybdA b0591 0.67 -0.2
622,409 - ybdA b0591 0.71 -0.8
622,434 + ybdA b0591 0.73 +0.3
622,441 - ybdA b0591 0.73 +1.1
622,441 - ybdA b0591 0.73 +0.5
622,472 + ybdA b0591 0.76 +0.1
622,472 + ybdA b0591 0.76 +0.4
622,491 + ybdA b0591 0.77 -0.0
622,596 + ybdA b0591 0.86 +0.8
622,596 + ybdA b0591 0.86 +0.0
622,611 - ybdA b0591 0.87 -0.2
622,611 - ybdA b0591 0.87 +0.5
622,615 + ybdA b0591 0.87 +0.1
622,615 + ybdA b0591 0.87 -0.2
622,801 - -0.5
622,819 + -0.6
622,819 + -0.2
622,847 - -0.1
622,875 + fepB b0592 0.10 -1.0
622,875 + fepB b0592 0.10 +0.7
622,899 + fepB b0592 0.13 +0.1
622,917 + fepB b0592 0.15 -0.5
622,923 + fepB b0592 0.15 -1.1
622,958 + fepB b0592 0.19 -0.1
623,019 + fepB b0592 0.25 +1.1
623,019 + fepB b0592 0.25 -0.7
623,028 - fepB b0592 0.26 -0.7
623,028 - fepB b0592 0.26 -0.2
623,032 + fepB b0592 0.27 +0.2
623,119 + fepB b0592 0.36 -0.3
623,140 - fepB b0592 0.38 -0.3
623,148 - fepB b0592 0.39 -0.5
623,148 - fepB b0592 0.39 -0.6
623,199 - fepB b0592 0.44 -0.6
623,253 - fepB b0592 0.50 +0.3
623,341 - fepB b0592 0.59 -2.5
623,405 - fepB b0592 0.66 -1.5
623,405 - fepB b0592 0.66 -1.7
623,426 + fepB b0592 0.68 -0.4
623,426 + fepB b0592 0.68 -0.4
623,458 - fepB b0592 0.71 -0.8
623,458 - fepB b0592 0.71 -1.2
623,467 - fepB b0592 0.72 -0.8
623,467 - fepB b0592 0.72 +0.1
623,470 - fepB b0592 0.72 -1.0
623,470 - fepB b0592 0.72 -0.3
623,563 + fepB b0592 0.82 -0.9
623,576 - fepB b0592 0.83 -0.8
623,576 - fepB b0592 0.83 +0.0
623,591 - fepB b0592 0.85 -0.8
623,591 - fepB b0592 0.85 -0.9
623,610 + fepB b0592 0.87 -0.5
623,672 + -0.0
623,708 + +0.1
623,806 + -0.6
623,822 + +0.3
623,822 + +0.6
623,830 - +0.1
623,832 - +0.1
623,841 + -0.1
623,857 + -2.0
623,865 + +0.3
623,869 + +0.2
623,869 + -0.3
623,879 - +0.0
623,907 - +0.8
623,907 - +0.1
624,008 + -0.0
624,130 - -0.2
624,130 - -0.2
624,138 - -0.2
624,138 - -0.6
624,259 + entC b0593 0.13 -0.2
624,428 - entC b0593 0.27 +0.3
624,428 - entC b0593 0.27 +0.5
624,490 + entC b0593 0.32 -0.5
624,547 - entC b0593 0.37 +1.6
624,580 - entC b0593 0.40 -0.4
624,691 + entC b0593 0.50 +0.3
624,691 + entC b0593 0.50 +0.1
624,699 - entC b0593 0.50 +0.1
624,699 - entC b0593 0.50 +0.2
624,699 - entC b0593 0.50 +0.6

Or see this region's nucleotide sequence