Experiment: 3hr_MSC_rep2_highinoculum
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt phoR and brnQ are separated by 406 nucleotides brnQ and proY are separated by 75 nucleotides
b0400: phoR - sensory histidine kinase in two-component regulatory system with PhoB (NCBI), at 417,113 to 418,408
phoR
b0401: brnQ - predicted branched chain amino acid transporter (LIV-II) (NCBI), at 418,815 to 420,134
brnQ
b0402: proY - predicted cryptic proline transporter (NCBI), at 420,210 to 421,583
proY
Position (kb) 418
419
420
421 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 417.889 kb on + strand, within phoR at 418.088 kb on + strand, within phoR at 418.096 kb on - strand, within phoR at 418.096 kb on - strand, within phoR at 418.144 kb on - strand, within phoR at 418.148 kb on + strand, within phoR at 418.175 kb on + strand, within phoR at 418.183 kb on - strand, within phoR at 418.183 kb on - strand, within phoR at 418.191 kb on + strand, within phoR at 418.191 kb on + strand, within phoR at 418.241 kb on + strand, within phoR at 418.283 kb on - strand at 418.327 kb on + strand at 418.333 kb on + strand at 418.333 kb on + strand at 418.336 kb on - strand at 418.346 kb on - strand at 418.423 kb on - strand at 418.611 kb on + strand at 418.658 kb on - strand at 418.794 kb on + strand at 418.856 kb on - strand at 418.899 kb on - strand at 418.899 kb on - strand at 418.915 kb on + strand at 418.942 kb on - strand at 418.949 kb on + strand, within brnQ at 418.969 kb on + strand, within brnQ at 418.992 kb on + strand, within brnQ at 419.112 kb on - strand, within brnQ at 419.153 kb on + strand, within brnQ at 419.195 kb on + strand, within brnQ at 419.243 kb on + strand, within brnQ at 419.249 kb on - strand, within brnQ at 419.263 kb on + strand, within brnQ at 419.263 kb on + strand, within brnQ at 419.308 kb on + strand, within brnQ at 419.328 kb on + strand, within brnQ at 419.404 kb on + strand, within brnQ at 419.526 kb on + strand, within brnQ at 419.542 kb on + strand, within brnQ at 419.545 kb on - strand, within brnQ at 419.545 kb on - strand, within brnQ at 419.547 kb on + strand, within brnQ at 419.547 kb on + strand, within brnQ at 419.645 kb on + strand, within brnQ at 419.703 kb on + strand, within brnQ at 419.703 kb on + strand, within brnQ at 419.703 kb on + strand, within brnQ at 419.709 kb on + strand, within brnQ at 419.733 kb on + strand, within brnQ at 419.746 kb on - strand, within brnQ at 419.746 kb on - strand, within brnQ at 419.763 kb on - strand, within brnQ at 419.817 kb on - strand, within brnQ at 419.909 kb on - strand, within brnQ at 419.909 kb on - strand, within brnQ at 420.005 kb on + strand at 420.119 kb on + strand at 420.119 kb on + strand at 420.234 kb on - strand at 420.260 kb on + strand at 420.339 kb on - strand at 420.364 kb on + strand, within proY at 420.364 kb on + strand, within proY at 420.374 kb on + strand, within proY at 420.382 kb on - strand, within proY at 420.382 kb on - strand, within proY at 420.410 kb on + strand, within proY at 420.410 kb on + strand, within proY at 420.545 kb on + strand, within proY at 420.545 kb on + strand, within proY at 420.553 kb on - strand, within proY at 420.553 kb on - strand, within proY at 420.573 kb on - strand, within proY at 420.573 kb on - strand, within proY at 420.573 kb on - strand, within proY at 420.573 kb on - strand, within proY at 420.588 kb on - strand, within proY at 420.624 kb on - strand, within proY at 420.694 kb on + strand, within proY at 420.694 kb on + strand, within proY at 420.723 kb on + strand, within proY at 420.934 kb on + strand, within proY at 420.944 kb on + strand, within proY at 420.984 kb on - strand, within proY at 421.003 kb on - strand, within proY at 421.036 kb on + strand, within proY at 421.037 kb on - strand, within proY at 421.126 kb on + strand, within proY
Per-strain Table
Position Strand Gene LocusTag Fraction 3hr_MSC_rep2_highinoculum remove 417,889 + phoR b0400 0.60 -0.3 418,088 + phoR b0400 0.75 +0.3 418,096 - phoR b0400 0.76 -0.5 418,096 - phoR b0400 0.76 +0.1 418,144 - phoR b0400 0.80 -0.4 418,148 + phoR b0400 0.80 -0.3 418,175 + phoR b0400 0.82 +0.4 418,183 - phoR b0400 0.83 +0.0 418,183 - phoR b0400 0.83 -0.3 418,191 + phoR b0400 0.83 +0.4 418,191 + phoR b0400 0.83 -0.2 418,241 + phoR b0400 0.87 -0.4 418,283 - -0.1 418,327 + -0.0 418,333 + +0.6 418,333 + -0.5 418,336 - -0.5 418,346 - -0.3 418,423 - -0.2 418,611 + -0.2 418,658 - -0.8 418,794 + +0.3 418,856 - -2.2 418,899 - -1.0 418,899 - -0.8 418,915 + -1.1 418,942 - -1.3 418,949 + brnQ b0401 0.10 +0.3 418,969 + brnQ b0401 0.12 -1.2 418,992 + brnQ b0401 0.13 -0.9 419,112 - brnQ b0401 0.23 -1.5 419,153 + brnQ b0401 0.26 -1.1 419,195 + brnQ b0401 0.29 -1.7 419,243 + brnQ b0401 0.32 -1.0 419,249 - brnQ b0401 0.33 -1.5 419,263 + brnQ b0401 0.34 -0.6 419,263 + brnQ b0401 0.34 -1.0 419,308 + brnQ b0401 0.37 -1.5 419,328 + brnQ b0401 0.39 -1.2 419,404 + brnQ b0401 0.45 -0.7 419,526 + brnQ b0401 0.54 -0.8 419,542 + brnQ b0401 0.55 -0.7 419,545 - brnQ b0401 0.55 -3.4 419,545 - brnQ b0401 0.55 +1.2 419,547 + brnQ b0401 0.55 -1.3 419,547 + brnQ b0401 0.55 -0.6 419,645 + brnQ b0401 0.63 -1.6 419,703 + brnQ b0401 0.67 -1.3 419,703 + brnQ b0401 0.67 -0.8 419,703 + brnQ b0401 0.67 -1.0 419,709 + brnQ b0401 0.68 -0.6 419,733 + brnQ b0401 0.70 -1.3 419,746 - brnQ b0401 0.71 -1.8 419,746 - brnQ b0401 0.71 -1.2 419,763 - brnQ b0401 0.72 -1.0 419,817 - brnQ b0401 0.76 -1.3 419,909 - brnQ b0401 0.83 -0.5 419,909 - brnQ b0401 0.83 -1.4 420,005 + -0.3 420,119 + -1.5 420,119 + -0.7 420,234 - -0.1 420,260 + -0.3 420,339 - -0.2 420,364 + proY b0402 0.11 +0.2 420,364 + proY b0402 0.11 +0.2 420,374 + proY b0402 0.12 -0.3 420,382 - proY b0402 0.13 -1.1 420,382 - proY b0402 0.13 -0.4 420,410 + proY b0402 0.15 -0.8 420,410 + proY b0402 0.15 +0.0 420,545 + proY b0402 0.24 +0.3 420,545 + proY b0402 0.24 -0.6 420,553 - proY b0402 0.25 -1.2 420,553 - proY b0402 0.25 -0.4 420,573 - proY b0402 0.26 +0.4 420,573 - proY b0402 0.26 +0.1 420,573 - proY b0402 0.26 -0.2 420,573 - proY b0402 0.26 -0.3 420,588 - proY b0402 0.28 -0.5 420,624 - proY b0402 0.30 -0.1 420,694 + proY b0402 0.35 -2.2 420,694 + proY b0402 0.35 -0.2 420,723 + proY b0402 0.37 -0.2 420,934 + proY b0402 0.53 -0.6 420,944 + proY b0402 0.53 -0.6 420,984 - proY b0402 0.56 +0.0 421,003 - proY b0402 0.58 +0.4 421,036 + proY b0402 0.60 +0.3 421,037 - proY b0402 0.60 -0.6 421,126 + proY b0402 0.67 -0.2
Or see this region's nucleotide sequence