Experiment: 3hr_MSC_rep1_highinoculum
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gcl and hyi are separated by 12 nucleotides hyi and glxR are separated by 99 nucleotides
b0507: gcl - glyoxylate carboligase (NCBI), at 533,140 to 534,921
gcl
b0508: hyi - hydroxypyruvate isomerase (NCBI), at 534,934 to 535,710
hyi
b0509: glxR - tartronate semialdehyde reductase, NADH-dependent (NCBI), at 535,810 to 536,688
glxR
Position (kb)
534
535
536 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 533.996 kb on - strand, within gcl at 534.135 kb on + strand, within gcl at 534.199 kb on - strand, within gcl at 534.217 kb on - strand, within gcl at 534.263 kb on + strand, within gcl at 534.279 kb on - strand, within gcl at 534.279 kb on - strand, within gcl at 534.281 kb on - strand, within gcl at 534.314 kb on + strand, within gcl at 534.539 kb on + strand, within gcl at 534.539 kb on + strand, within gcl at 534.544 kb on - strand, within gcl at 534.557 kb on + strand, within gcl at 534.557 kb on + strand, within gcl at 534.571 kb on - strand, within gcl at 534.661 kb on + strand, within gcl at 534.670 kb on - strand, within gcl at 534.672 kb on + strand, within gcl at 534.696 kb on + strand, within gcl at 534.740 kb on + strand, within gcl at 534.740 kb on + strand, within gcl at 534.740 kb on + strand, within gcl at 534.756 kb on + strand at 534.756 kb on + strand at 534.830 kb on + strand at 534.830 kb on + strand at 534.830 kb on + strand at 534.833 kb on + strand at 534.833 kb on + strand at 534.835 kb on + strand at 534.835 kb on + strand at 534.843 kb on - strand at 534.865 kb on + strand at 534.868 kb on + strand at 534.868 kb on + strand at 534.868 kb on + strand at 534.878 kb on + strand at 534.878 kb on + strand at 534.889 kb on + strand at 534.891 kb on + strand at 534.891 kb on + strand at 534.892 kb on + strand at 534.892 kb on + strand at 534.899 kb on - strand at 534.911 kb on + strand at 534.912 kb on + strand at 534.912 kb on + strand at 535.001 kb on + strand at 535.011 kb on + strand at 535.128 kb on - strand, within hyi at 535.143 kb on - strand, within hyi at 535.159 kb on + strand, within hyi at 535.162 kb on - strand, within hyi at 535.162 kb on - strand, within hyi at 535.207 kb on + strand, within hyi at 535.207 kb on + strand, within hyi at 535.207 kb on + strand, within hyi at 535.214 kb on + strand, within hyi at 535.214 kb on + strand, within hyi at 535.267 kb on + strand, within hyi at 535.356 kb on + strand, within hyi at 535.403 kb on + strand, within hyi at 535.435 kb on + strand, within hyi at 535.485 kb on + strand, within hyi at 535.511 kb on + strand, within hyi at 535.532 kb on - strand, within hyi at 535.681 kb on + strand at 535.686 kb on + strand at 535.691 kb on + strand at 535.691 kb on + strand at 535.696 kb on + strand at 535.704 kb on - strand at 535.717 kb on + strand at 535.717 kb on + strand at 535.722 kb on + strand at 535.759 kb on - strand at 535.759 kb on - strand at 535.760 kb on + strand at 535.760 kb on + strand at 535.760 kb on + strand at 535.841 kb on + strand at 535.849 kb on - strand at 535.860 kb on - strand at 535.882 kb on + strand at 535.890 kb on - strand at 535.892 kb on - strand at 535.952 kb on - strand, within glxR at 535.967 kb on - strand, within glxR at 535.969 kb on - strand, within glxR at 535.998 kb on + strand, within glxR at 536.006 kb on + strand, within glxR at 536.007 kb on + strand, within glxR at 536.007 kb on + strand, within glxR at 536.048 kb on - strand, within glxR at 536.128 kb on + strand, within glxR at 536.174 kb on + strand, within glxR at 536.187 kb on + strand, within glxR at 536.195 kb on - strand, within glxR at 536.295 kb on - strand, within glxR at 536.308 kb on - strand, within glxR at 536.393 kb on - strand, within glxR at 536.429 kb on + strand, within glxR at 536.492 kb on - strand, within glxR at 536.522 kb on + strand, within glxR at 536.533 kb on + strand, within glxR at 536.533 kb on + strand, within glxR at 536.536 kb on - strand, within glxR at 536.595 kb on + strand, within glxR at 536.595 kb on + strand, within glxR at 536.626 kb on + strand at 536.626 kb on + strand at 536.631 kb on + strand at 536.636 kb on + strand at 536.637 kb on - strand at 536.639 kb on + strand at 536.641 kb on + strand at 536.641 kb on + strand at 536.641 kb on + strand at 536.641 kb on + strand at 536.658 kb on + strand at 536.658 kb on + strand at 536.672 kb on + strand at 536.672 kb on + strand at 536.672 kb on + strand at 536.672 kb on + strand at 536.672 kb on + strand at 536.672 kb on + strand at 536.672 kb on + strand at 536.672 kb on + strand at 536.672 kb on + strand at 536.672 kb on + strand at 536.672 kb on + strand at 536.672 kb on + strand at 536.672 kb on + strand at 536.672 kb on + strand at 536.673 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 3hr_MSC_rep1_highinoculum remove 533,996 - gcl b0507 0.48 +0.0 534,135 + gcl b0507 0.56 -0.2 534,199 - gcl b0507 0.59 +0.3 534,217 - gcl b0507 0.60 -0.7 534,263 + gcl b0507 0.63 -0.2 534,279 - gcl b0507 0.64 -0.2 534,279 - gcl b0507 0.64 +0.6 534,281 - gcl b0507 0.64 +0.1 534,314 + gcl b0507 0.66 -0.1 534,539 + gcl b0507 0.79 -0.1 534,539 + gcl b0507 0.79 +0.6 534,544 - gcl b0507 0.79 -2.1 534,557 + gcl b0507 0.80 +0.5 534,557 + gcl b0507 0.80 -0.9 534,571 - gcl b0507 0.80 +0.9 534,661 + gcl b0507 0.85 -0.2 534,670 - gcl b0507 0.86 +2.6 534,672 + gcl b0507 0.86 -0.0 534,696 + gcl b0507 0.87 +0.5 534,740 + gcl b0507 0.90 -0.8 534,740 + gcl b0507 0.90 -1.6 534,740 + gcl b0507 0.90 -0.0 534,756 + -0.3 534,756 + -0.2 534,830 + -0.3 534,830 + -0.8 534,830 + +0.5 534,833 + -0.3 534,833 + -0.4 534,835 + +0.8 534,835 + +0.2 534,843 - +2.6 534,865 + +0.0 534,868 + -0.3 534,868 + +0.2 534,868 + +0.6 534,878 + -0.8 534,878 + -1.1 534,889 + -1.0 534,891 + -0.1 534,891 + -0.2 534,892 + +0.3 534,892 + +0.8 534,899 - +2.6 534,911 + -0.1 534,912 + +0.1 534,912 + -0.1 535,001 + +1.2 535,011 + +0.3 535,128 - hyi b0508 0.25 +0.6 535,143 - hyi b0508 0.27 +0.6 535,159 + hyi b0508 0.29 +1.6 535,162 - hyi b0508 0.29 +2.6 535,162 - hyi b0508 0.29 +2.6 535,207 + hyi b0508 0.35 -2.1 535,207 + hyi b0508 0.35 +0.5 535,207 + hyi b0508 0.35 +1.6 535,214 + hyi b0508 0.36 -0.6 535,214 + hyi b0508 0.36 -0.2 535,267 + hyi b0508 0.43 -0.7 535,356 + hyi b0508 0.54 +0.3 535,403 + hyi b0508 0.60 -0.7 535,435 + hyi b0508 0.64 -1.3 535,485 + hyi b0508 0.71 +2.6 535,511 + hyi b0508 0.74 -0.0 535,532 - hyi b0508 0.77 +2.6 535,681 + +0.5 535,686 + +0.4 535,691 + -0.1 535,691 + -1.6 535,696 + +1.1 535,704 - -0.8 535,717 + -0.0 535,717 + -0.0 535,722 + -0.1 535,759 - +0.6 535,759 - +2.1 535,760 + +0.6 535,760 + +0.2 535,760 + +0.2 535,841 + +5.0 535,849 - +0.1 535,860 - -1.5 535,882 + -0.5 535,890 - +0.6 535,892 - +0.1 535,952 - glxR b0509 0.16 +0.3 535,967 - glxR b0509 0.18 +0.6 535,969 - glxR b0509 0.18 +0.1 535,998 + glxR b0509 0.21 +0.4 536,006 + glxR b0509 0.22 +0.2 536,007 + glxR b0509 0.22 +0.1 536,007 + glxR b0509 0.22 +0.3 536,048 - glxR b0509 0.27 -0.5 536,128 + glxR b0509 0.36 -0.4 536,174 + glxR b0509 0.41 -0.3 536,187 + glxR b0509 0.43 -0.0 536,195 - glxR b0509 0.44 -0.9 536,295 - glxR b0509 0.55 +1.3 536,308 - glxR b0509 0.57 -0.1 536,393 - glxR b0509 0.66 +1.1 536,429 + glxR b0509 0.70 -0.4 536,492 - glxR b0509 0.78 +1.3 536,522 + glxR b0509 0.81 -0.2 536,533 + glxR b0509 0.82 -0.1 536,533 + glxR b0509 0.82 +0.1 536,536 - glxR b0509 0.83 +2.6 536,595 + glxR b0509 0.89 +0.1 536,595 + glxR b0509 0.89 -0.5 536,626 + -0.2 536,626 + -0.2 536,631 + +0.5 536,636 + +0.2 536,637 - +2.6 536,639 + -0.3 536,641 + +0.4 536,641 + -0.2 536,641 + -0.2 536,641 + +0.1 536,658 + -0.3 536,658 + +0.6 536,672 + -0.2 536,672 + +0.2 536,672 + -0.1 536,672 + +0.3 536,672 + +0.1 536,672 + -0.2 536,672 + -0.3 536,672 + -0.1 536,672 + -0.1 536,672 + -0.0 536,672 + -0.3 536,672 + -0.3 536,672 + +0.5 536,672 + -1.3 536,673 + -0.1
Or see this region's nucleotide sequence