Strain Fitness in Escherichia coli BW25113 around b4408

Experiment: RCH2_defined_1percent_glucose with Chloride 200 mM and Betaine 100 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyqcB and yqcC overlap by 1 nucleotidesyqcC and csrB are separated by 42 nucleotidescsrB and syd are separated by 210 nucleotidessyd and yqcD are separated by 67 nucleotides b2791: yqcB - tRNA pseudouridine synthase (NCBI), at 2,921,024 to 2,921,806 yqcB b2792: yqcC - hypothetical protein (NCBI), at 2,921,806 to 2,922,135 yqcC b4408: csrB - CsrA-binding sRNA, antagonizing CsrA regulation (NCBI), at 2,922,178 to 2,922,546 csrB b2793: syd - SecY interacting protein Syd (NCBI), at 2,922,757 to 2,923,302 syd b2794: yqcD - hypothetical protein (NCBI), at 2,923,370 to 2,924,218 yqcD Position (kb) 2922 2923Strain fitness (log2 ratio) -3 -2 -1 0 1at 2921.209 kb on - strand, within yqcBat 2921.317 kb on + strand, within yqcBat 2921.351 kb on + strand, within yqcBat 2921.504 kb on + strand, within yqcBat 2921.504 kb on + strand, within yqcBat 2921.547 kb on - strand, within yqcBat 2921.631 kb on - strand, within yqcBat 2921.637 kb on - strand, within yqcBat 2921.637 kb on - strand, within yqcBat 2921.710 kb on - strand, within yqcBat 2921.710 kb on - strand, within yqcBat 2921.785 kb on - strandat 2921.795 kb on - strandat 2921.795 kb on - strandat 2921.820 kb on - strandat 2921.882 kb on + strand, within yqcCat 2921.980 kb on - strand, within yqcCat 2921.980 kb on - strand, within yqcCat 2921.992 kb on + strand, within yqcCat 2922.015 kb on - strand, within yqcCat 2922.118 kb on - strandat 2922.131 kb on + strandat 2922.194 kb on + strandat 2922.204 kb on - strandat 2922.317 kb on - strand, within csrBat 2922.407 kb on + strand, within csrBat 2922.407 kb on + strand, within csrBat 2922.418 kb on + strand, within csrBat 2922.443 kb on - strand, within csrBat 2922.488 kb on - strand, within csrBat 2922.501 kb on - strand, within csrBat 2922.544 kb on + strandat 2922.573 kb on + strandat 2922.573 kb on + strandat 2922.686 kb on - strandat 2922.705 kb on + strandat 2922.705 kb on + strandat 2922.717 kb on - strandat 2922.717 kb on - strandat 2922.846 kb on + strand, within sydat 2922.848 kb on + strand, within sydat 2922.848 kb on + strand, within sydat 2922.859 kb on + strand, within sydat 2922.859 kb on + strand, within sydat 2922.869 kb on + strand, within sydat 2922.892 kb on - strand, within sydat 2922.929 kb on - strand, within sydat 2922.946 kb on - strand, within sydat 2922.970 kb on - strand, within sydat 2922.982 kb on - strand, within sydat 2923.017 kb on - strand, within sydat 2923.017 kb on - strand, within sydat 2923.042 kb on - strand, within sydat 2923.048 kb on + strand, within sydat 2923.048 kb on + strand, within sydat 2923.066 kb on + strand, within sydat 2923.139 kb on + strand, within sydat 2923.161 kb on - strand, within sydat 2923.161 kb on - strand, within sydat 2923.161 kb on - strand, within sydat 2923.207 kb on - strand, within sydat 2923.225 kb on + strand, within sydat 2923.233 kb on - strand, within sydat 2923.235 kb on + strand, within sydat 2923.235 kb on + strand, within sydat 2923.255 kb on + strandat 2923.301 kb on + strandat 2923.361 kb on - strandat 2923.361 kb on - strandat 2923.454 kb on - strandat 2923.460 kb on - strand, within yqcDat 2923.537 kb on - strand, within yqcDat 2923.537 kb on - strand, within yqcD

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_1percent_glucose with Chloride 200 mM and Betaine 100 uM
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2,921,209 - yqcB b2791 0.24 +0.4
2,921,317 + yqcB b2791 0.37 -0.6
2,921,351 + yqcB b2791 0.42 +0.7
2,921,504 + yqcB b2791 0.61 +0.2
2,921,504 + yqcB b2791 0.61 +0.7
2,921,547 - yqcB b2791 0.67 -0.3
2,921,631 - yqcB b2791 0.78 +0.1
2,921,637 - yqcB b2791 0.78 -0.0
2,921,637 - yqcB b2791 0.78 -0.5
2,921,710 - yqcB b2791 0.88 -0.2
2,921,710 - yqcB b2791 0.88 +0.3
2,921,785 - -0.5
2,921,795 - +1.1
2,921,795 - +1.1
2,921,820 - +0.2
2,921,882 + yqcC b2792 0.23 +0.4
2,921,980 - yqcC b2792 0.53 +0.5
2,921,980 - yqcC b2792 0.53 +0.5
2,921,992 + yqcC b2792 0.56 +0.2
2,922,015 - yqcC b2792 0.63 +0.3
2,922,118 - +0.2
2,922,131 + -0.1
2,922,194 + -0.7
2,922,204 - -0.2
2,922,317 - csrB b4408 0.38 +0.5
2,922,407 + csrB b4408 0.62 +1.5
2,922,407 + csrB b4408 0.62 +0.1
2,922,418 + csrB b4408 0.65 -0.7
2,922,443 - csrB b4408 0.72 +0.1
2,922,488 - csrB b4408 0.84 +1.0
2,922,501 - csrB b4408 0.88 +1.0
2,922,544 + -1.4
2,922,573 + -0.9
2,922,573 + +0.0
2,922,686 - -0.0
2,922,705 + +0.3
2,922,705 + +0.2
2,922,717 - -0.3
2,922,717 - +0.3
2,922,846 + syd b2793 0.16 -0.3
2,922,848 + syd b2793 0.17 +0.5
2,922,848 + syd b2793 0.17 +0.1
2,922,859 + syd b2793 0.19 -0.4
2,922,859 + syd b2793 0.19 -0.0
2,922,869 + syd b2793 0.21 +0.2
2,922,892 - syd b2793 0.25 -0.2
2,922,929 - syd b2793 0.32 +0.1
2,922,946 - syd b2793 0.35 -0.8
2,922,970 - syd b2793 0.39 -0.2
2,922,982 - syd b2793 0.41 +0.3
2,923,017 - syd b2793 0.48 -0.1
2,923,017 - syd b2793 0.48 -0.8
2,923,042 - syd b2793 0.52 +0.7
2,923,048 + syd b2793 0.53 -0.6
2,923,048 + syd b2793 0.53 +0.7
2,923,066 + syd b2793 0.57 -0.0
2,923,139 + syd b2793 0.70 +0.6
2,923,161 - syd b2793 0.74 +1.2
2,923,161 - syd b2793 0.74 +0.4
2,923,161 - syd b2793 0.74 +0.2
2,923,207 - syd b2793 0.82 -0.3
2,923,225 + syd b2793 0.86 +0.1
2,923,233 - syd b2793 0.87 -0.1
2,923,235 + syd b2793 0.88 -0.7
2,923,235 + syd b2793 0.88 -2.1
2,923,255 + +0.3
2,923,301 + -2.8
2,923,361 - -1.8
2,923,361 - -0.1
2,923,454 - -0.4
2,923,460 - yqcD b2794 0.11 -0.6
2,923,537 - yqcD b2794 0.20 -1.5
2,923,537 - yqcD b2794 0.20 +0.4

Or see this region's nucleotide sequence