Strain Fitness in Escherichia coli BW25113 around b4076

Experiment: RCH2_defined_1percent_glucose with Chloride 200 mM and Betaine 100 uM

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntnrfE and nrfF overlap by 8 nucleotidesnrfF and nrfG overlap by 4 nucleotidesnrfG and gltP are separated by 341 nucleotides b4074: nrfE - heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfE (NCBI), at 4,289,535 to 4,291,193 nrfE b4075: nrfF - heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfF (NCBI), at 4,291,186 to 4,291,569 nrfF b4076: nrfG - heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfG (NCBI), at 4,291,566 to 4,292,162 nrfG b4077: gltP - glutamate/aspartate:proton symporter (NCBI), at 4,292,504 to 4,293,817 gltP Position (kb) 4291 4292 4293Strain fitness (log2 ratio) -3 -2 -1 0 1at 4290.566 kb on + strand, within nrfEat 4290.572 kb on - strand, within nrfEat 4290.619 kb on + strand, within nrfEat 4290.619 kb on + strand, within nrfEat 4290.726 kb on + strand, within nrfEat 4290.797 kb on - strand, within nrfEat 4290.832 kb on + strand, within nrfEat 4290.839 kb on + strand, within nrfEat 4290.839 kb on + strand, within nrfEat 4290.847 kb on - strand, within nrfEat 4290.930 kb on + strand, within nrfEat 4290.952 kb on + strand, within nrfEat 4291.075 kb on + strandat 4291.075 kb on + strandat 4291.155 kb on + strandat 4291.208 kb on - strandat 4291.236 kb on + strand, within nrfFat 4291.270 kb on + strand, within nrfFat 4291.423 kb on - strand, within nrfFat 4291.464 kb on + strand, within nrfFat 4291.472 kb on - strand, within nrfFat 4291.483 kb on + strand, within nrfFat 4291.497 kb on + strand, within nrfFat 4291.503 kb on + strand, within nrfFat 4291.503 kb on + strand, within nrfFat 4291.711 kb on - strand, within nrfGat 4291.800 kb on - strand, within nrfGat 4291.854 kb on + strand, within nrfGat 4291.859 kb on + strand, within nrfGat 4291.911 kb on + strand, within nrfGat 4291.911 kb on + strand, within nrfGat 4291.934 kb on + strand, within nrfGat 4291.982 kb on + strand, within nrfGat 4291.982 kb on + strand, within nrfGat 4292.106 kb on + strandat 4292.109 kb on + strandat 4292.112 kb on - strandat 4292.112 kb on - strandat 4292.114 kb on + strandat 4292.114 kb on + strandat 4292.131 kb on - strandat 4292.368 kb on + strandat 4292.368 kb on + strandat 4292.388 kb on + strandat 4292.479 kb on + strandat 4292.479 kb on + strandat 4292.479 kb on + strandat 4292.479 kb on + strandat 4292.479 kb on + strandat 4292.574 kb on + strandat 4292.594 kb on - strandat 4292.594 kb on - strandat 4292.699 kb on - strand, within gltPat 4292.771 kb on + strand, within gltPat 4292.771 kb on + strand, within gltPat 4292.792 kb on - strand, within gltPat 4292.851 kb on + strand, within gltPat 4292.851 kb on + strand, within gltPat 4292.897 kb on + strand, within gltPat 4292.897 kb on + strand, within gltPat 4292.897 kb on + strand, within gltPat 4292.960 kb on - strand, within gltPat 4293.079 kb on + strand, within gltPat 4293.115 kb on + strand, within gltPat 4293.115 kb on + strand, within gltP

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_1percent_glucose with Chloride 200 mM and Betaine 100 uM
remove
4,290,566 + nrfE b4074 0.62 -0.1
4,290,572 - nrfE b4074 0.63 +0.8
4,290,619 + nrfE b4074 0.65 +0.7
4,290,619 + nrfE b4074 0.65 -0.5
4,290,726 + nrfE b4074 0.72 +0.3
4,290,797 - nrfE b4074 0.76 -0.0
4,290,832 + nrfE b4074 0.78 +0.0
4,290,839 + nrfE b4074 0.79 -1.1
4,290,839 + nrfE b4074 0.79 -0.7
4,290,847 - nrfE b4074 0.79 +0.2
4,290,930 + nrfE b4074 0.84 -0.3
4,290,952 + nrfE b4074 0.85 -3.7
4,291,075 + +0.1
4,291,075 + +0.6
4,291,155 + -0.2
4,291,208 - +0.3
4,291,236 + nrfF b4075 0.13 +0.8
4,291,270 + nrfF b4075 0.22 -0.9
4,291,423 - nrfF b4075 0.62 -1.4
4,291,464 + nrfF b4075 0.72 +0.6
4,291,472 - nrfF b4075 0.74 +0.2
4,291,483 + nrfF b4075 0.77 +0.5
4,291,497 + nrfF b4075 0.81 -0.4
4,291,503 + nrfF b4075 0.83 -0.1
4,291,503 + nrfF b4075 0.83 -0.4
4,291,711 - nrfG b4076 0.24 +0.3
4,291,800 - nrfG b4076 0.39 +0.1
4,291,854 + nrfG b4076 0.48 +0.2
4,291,859 + nrfG b4076 0.49 +0.1
4,291,911 + nrfG b4076 0.58 +1.1
4,291,911 + nrfG b4076 0.58 -1.7
4,291,934 + nrfG b4076 0.62 +1.6
4,291,982 + nrfG b4076 0.70 +0.6
4,291,982 + nrfG b4076 0.70 -0.1
4,292,106 + -0.7
4,292,109 + -0.1
4,292,112 - -0.3
4,292,112 - -0.9
4,292,114 + -2.2
4,292,114 + +0.0
4,292,131 - -3.1
4,292,368 + +0.3
4,292,368 + +0.5
4,292,388 + +0.4
4,292,479 + -0.1
4,292,479 + -0.6
4,292,479 + +0.3
4,292,479 + -0.2
4,292,479 + -0.3
4,292,574 + +0.3
4,292,594 - -0.4
4,292,594 - -0.7
4,292,699 - gltP b4077 0.15 -1.8
4,292,771 + gltP b4077 0.20 +0.0
4,292,771 + gltP b4077 0.20 -0.3
4,292,792 - gltP b4077 0.22 -1.5
4,292,851 + gltP b4077 0.26 -0.4
4,292,851 + gltP b4077 0.26 -0.1
4,292,897 + gltP b4077 0.30 +1.0
4,292,897 + gltP b4077 0.30 +0.9
4,292,897 + gltP b4077 0.30 -0.3
4,292,960 - gltP b4077 0.35 -0.2
4,293,079 + gltP b4077 0.44 -0.2
4,293,115 + gltP b4077 0.46 -0.4
4,293,115 + gltP b4077 0.46 +0.4

Or see this region's nucleotide sequence