Strain Fitness in Escherichia coli BW25113 around b3052

Experiment: RCH2_defined_1percent_glucose with Chloride 200 mM and Betaine 100 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyqiK and rygD are separated by 197 nucleotidesrygD and ibsD overlap by 100 nucleotidesibsD and rygE are separated by 273 nucleotidesrygE and ibsE overlap by 100 nucleotidesibsE and rfaE are separated by 119 nucleotidesrfaE and glnE are separated by 47 nucleotides b3051: yqiK - hypothetical protein (NCBI), at 3,190,886 to 3,192,547 yqiK b4447: rygD - Putative sRNA, function unknown; paralogous to the other QUAD sRNA genes (RefSeq), at 3,192,745 to 3,192,887 rygD b4664: ibsD - toxic membrane protein (RefSeq), at 3,192,788 to 3,192,847 ibsD b4611: rygE - Putative sRNA, function unknown; paralogous to the other QUAD sRNA genes (RefSeq), at 3,193,121 to 3,193,262 rygE b4666: ibsE - toxic membrane protein (RefSeq), at 3,193,163 to 3,193,222 ibsE b3052: rfaE - fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI), at 3,193,342 to 3,194,775 rfaE b3053: glnE - fused deadenylyltransferase/adenylyltransferase for glutamine synthetase (NCBI), at 3,194,823 to 3,197,663 glnE Position (kb) 3193 3194 3195Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 3192.427 kb on - strandat 3192.563 kb on - strandat 3192.698 kb on - strandat 3192.698 kb on - strandat 3192.740 kb on - strandat 3192.943 kb on + strandat 3193.398 kb on + strandat 3193.441 kb on - strandat 3193.545 kb on + strand, within rfaEat 3193.553 kb on - strand, within rfaEat 3193.583 kb on + strand, within rfaEat 3193.736 kb on + strand, within rfaEat 3193.771 kb on - strand, within rfaEat 3193.924 kb on - strand, within rfaEat 3193.930 kb on - strand, within rfaEat 3194.000 kb on + strand, within rfaEat 3194.000 kb on + strand, within rfaEat 3194.010 kb on + strand, within rfaEat 3194.010 kb on - strand, within rfaEat 3194.059 kb on - strand, within rfaEat 3194.059 kb on - strand, within rfaEat 3194.063 kb on + strand, within rfaEat 3194.071 kb on - strand, within rfaEat 3194.092 kb on - strand, within rfaEat 3194.151 kb on - strand, within rfaEat 3194.213 kb on + strand, within rfaEat 3194.221 kb on - strand, within rfaEat 3194.300 kb on - strandat 3194.300 kb on - strand, within rfaEat 3194.300 kb on - strand, within rfaEat 3194.396 kb on - strand, within rfaEat 3194.430 kb on + strand, within rfaEat 3194.495 kb on - strand, within rfaEat 3194.556 kb on - strand, within rfaEat 3194.556 kb on - strand, within rfaEat 3194.585 kb on - strand, within rfaEat 3194.618 kb on - strand, within rfaEat 3194.631 kb on - strand, within rfaEat 3194.768 kb on + strandat 3194.768 kb on + strandat 3194.790 kb on - strandat 3194.821 kb on - strandat 3194.821 kb on - strandat 3194.824 kb on - strandat 3194.954 kb on + strandat 3194.958 kb on - strandat 3194.958 kb on - strandat 3194.964 kb on - strandat 3194.964 kb on - strandat 3194.992 kb on - strandat 3195.011 kb on + strandat 3195.011 kb on + strandat 3195.069 kb on + strandat 3195.130 kb on + strand, within glnEat 3195.130 kb on + strand, within glnEat 3195.130 kb on + strand, within glnEat 3195.208 kb on + strand, within glnEat 3195.208 kb on + strand, within glnEat 3195.287 kb on + strand, within glnEat 3195.385 kb on + strand, within glnEat 3195.442 kb on - strand, within glnEat 3195.442 kb on - strand, within glnEat 3195.463 kb on - strand, within glnEat 3195.478 kb on + strand, within glnEat 3195.478 kb on + strand, within glnEat 3195.479 kb on + strand, within glnEat 3195.555 kb on + strand, within glnEat 3195.581 kb on - strand, within glnEat 3195.718 kb on + strand, within glnEat 3195.731 kb on - strand, within glnE

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_1percent_glucose with Chloride 200 mM and Betaine 100 uM
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3,192,427 - +1.3
3,192,563 - -1.1
3,192,698 - +0.7
3,192,698 - -0.3
3,192,740 - -0.0
3,192,943 + +0.4
3,193,398 + +2.3
3,193,441 - +1.1
3,193,545 + rfaE b3052 0.14 +1.3
3,193,553 - rfaE b3052 0.15 +0.7
3,193,583 + rfaE b3052 0.17 -1.7
3,193,736 + rfaE b3052 0.27 +1.0
3,193,771 - rfaE b3052 0.30 +1.7
3,193,924 - rfaE b3052 0.41 +0.1
3,193,930 - rfaE b3052 0.41 +0.7
3,194,000 + rfaE b3052 0.46 +1.3
3,194,000 + rfaE b3052 0.46 +0.9
3,194,010 + rfaE b3052 0.47 +1.0
3,194,010 - rfaE b3052 0.47 +1.1
3,194,059 - rfaE b3052 0.50 -0.9
3,194,059 - rfaE b3052 0.50 +1.5
3,194,063 + rfaE b3052 0.50 +1.3
3,194,071 - rfaE b3052 0.51 +0.8
3,194,092 - rfaE b3052 0.52 +0.3
3,194,151 - rfaE b3052 0.56 -1.9
3,194,213 + rfaE b3052 0.61 +1.5
3,194,221 - rfaE b3052 0.61 +0.7
3,194,300 - +1.3
3,194,300 - rfaE b3052 0.67 +0.6
3,194,300 - rfaE b3052 0.67 +1.7
3,194,396 - rfaE b3052 0.74 +4.7
3,194,430 + rfaE b3052 0.76 +1.9
3,194,495 - rfaE b3052 0.80 +0.5
3,194,556 - rfaE b3052 0.85 +1.6
3,194,556 - rfaE b3052 0.85 +1.0
3,194,585 - rfaE b3052 0.87 +2.4
3,194,618 - rfaE b3052 0.89 +1.2
3,194,631 - rfaE b3052 0.90 +1.4
3,194,768 + +0.0
3,194,768 + +0.5
3,194,790 - -0.6
3,194,821 - +0.0
3,194,821 - +0.2
3,194,824 - +0.2
3,194,954 + +0.8
3,194,958 - +0.9
3,194,958 - +0.0
3,194,964 - -0.1
3,194,964 - +0.5
3,194,992 - +0.3
3,195,011 + -0.1
3,195,011 + -0.7
3,195,069 + +0.1
3,195,130 + glnE b3053 0.11 +0.0
3,195,130 + glnE b3053 0.11 +1.2
3,195,130 + glnE b3053 0.11 +0.4
3,195,208 + glnE b3053 0.14 -0.0
3,195,208 + glnE b3053 0.14 +1.1
3,195,287 + glnE b3053 0.16 +0.3
3,195,385 + glnE b3053 0.20 +0.4
3,195,442 - glnE b3053 0.22 -0.1
3,195,442 - glnE b3053 0.22 +1.3
3,195,463 - glnE b3053 0.23 +0.9
3,195,478 + glnE b3053 0.23 +0.3
3,195,478 + glnE b3053 0.23 -0.9
3,195,479 + glnE b3053 0.23 -0.1
3,195,555 + glnE b3053 0.26 +0.1
3,195,581 - glnE b3053 0.27 -0.2
3,195,718 + glnE b3053 0.32 -0.1
3,195,731 - glnE b3053 0.32 -0.2

Or see this region's nucleotide sequence