Experiment: RCH2_defined_1percent_glucose with Chloride 200 mM and Betaine 100 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yqiK and rygD are separated by 197 nucleotides rygD and ibsD overlap by 100 nucleotides ibsD and rygE are separated by 273 nucleotides rygE and ibsE overlap by 100 nucleotides ibsE and rfaE are separated by 119 nucleotides rfaE and glnE are separated by 47 nucleotides
b3051: yqiK - hypothetical protein (NCBI), at 3,190,886 to 3,192,547
yqiK
b4447: rygD - Putative sRNA, function unknown; paralogous to the other QUAD sRNA genes (RefSeq), at 3,192,745 to 3,192,887
rygD
b4664: ibsD - toxic membrane protein (RefSeq), at 3,192,788 to 3,192,847
ibsD
b4611: rygE - Putative sRNA, function unknown; paralogous to the other QUAD sRNA genes (RefSeq), at 3,193,121 to 3,193,262
rygE
b4666: ibsE - toxic membrane protein (RefSeq), at 3,193,163 to 3,193,222
ibsE
b3052: rfaE - fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI), at 3,193,342 to 3,194,775
rfaE
b3053: glnE - fused deadenylyltransferase/adenylyltransferase for glutamine synthetase (NCBI), at 3,194,823 to 3,197,663
glnE
Position (kb)
3193
3194
3195 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3192.427 kb on - strand at 3192.563 kb on - strand at 3192.698 kb on - strand at 3192.698 kb on - strand at 3192.740 kb on - strand at 3192.943 kb on + strand at 3193.398 kb on + strand at 3193.441 kb on - strand at 3193.545 kb on + strand, within rfaE at 3193.553 kb on - strand, within rfaE at 3193.583 kb on + strand, within rfaE at 3193.736 kb on + strand, within rfaE at 3193.771 kb on - strand, within rfaE at 3193.924 kb on - strand, within rfaE at 3193.930 kb on - strand, within rfaE at 3194.000 kb on + strand, within rfaE at 3194.000 kb on + strand, within rfaE at 3194.010 kb on + strand, within rfaE at 3194.010 kb on - strand, within rfaE at 3194.059 kb on - strand, within rfaE at 3194.059 kb on - strand, within rfaE at 3194.063 kb on + strand, within rfaE at 3194.071 kb on - strand, within rfaE at 3194.092 kb on - strand, within rfaE at 3194.151 kb on - strand, within rfaE at 3194.213 kb on + strand, within rfaE at 3194.221 kb on - strand, within rfaE at 3194.300 kb on - strand at 3194.300 kb on - strand, within rfaE at 3194.300 kb on - strand, within rfaE at 3194.396 kb on - strand, within rfaE at 3194.430 kb on + strand, within rfaE at 3194.495 kb on - strand, within rfaE at 3194.556 kb on - strand, within rfaE at 3194.556 kb on - strand, within rfaE at 3194.585 kb on - strand, within rfaE at 3194.618 kb on - strand, within rfaE at 3194.631 kb on - strand, within rfaE at 3194.768 kb on + strand at 3194.768 kb on + strand at 3194.790 kb on - strand at 3194.821 kb on - strand at 3194.821 kb on - strand at 3194.824 kb on - strand at 3194.954 kb on + strand at 3194.958 kb on - strand at 3194.958 kb on - strand at 3194.964 kb on - strand at 3194.964 kb on - strand at 3194.992 kb on - strand at 3195.011 kb on + strand at 3195.011 kb on + strand at 3195.069 kb on + strand at 3195.130 kb on + strand, within glnE at 3195.130 kb on + strand, within glnE at 3195.130 kb on + strand, within glnE at 3195.208 kb on + strand, within glnE at 3195.208 kb on + strand, within glnE at 3195.287 kb on + strand, within glnE at 3195.385 kb on + strand, within glnE at 3195.442 kb on - strand, within glnE at 3195.442 kb on - strand, within glnE at 3195.463 kb on - strand, within glnE at 3195.478 kb on + strand, within glnE at 3195.478 kb on + strand, within glnE at 3195.479 kb on + strand, within glnE at 3195.555 kb on + strand, within glnE at 3195.581 kb on - strand, within glnE at 3195.718 kb on + strand, within glnE at 3195.731 kb on - strand, within glnE
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_1percent_glucose with Chloride 200 mM and Betaine 100 uM remove 3,192,427 - +1.3 3,192,563 - -1.1 3,192,698 - +0.7 3,192,698 - -0.3 3,192,740 - -0.0 3,192,943 + +0.4 3,193,398 + +2.3 3,193,441 - +1.1 3,193,545 + rfaE b3052 0.14 +1.3 3,193,553 - rfaE b3052 0.15 +0.7 3,193,583 + rfaE b3052 0.17 -1.7 3,193,736 + rfaE b3052 0.27 +1.0 3,193,771 - rfaE b3052 0.30 +1.7 3,193,924 - rfaE b3052 0.41 +0.1 3,193,930 - rfaE b3052 0.41 +0.7 3,194,000 + rfaE b3052 0.46 +1.3 3,194,000 + rfaE b3052 0.46 +0.9 3,194,010 + rfaE b3052 0.47 +1.0 3,194,010 - rfaE b3052 0.47 +1.1 3,194,059 - rfaE b3052 0.50 -0.9 3,194,059 - rfaE b3052 0.50 +1.5 3,194,063 + rfaE b3052 0.50 +1.3 3,194,071 - rfaE b3052 0.51 +0.8 3,194,092 - rfaE b3052 0.52 +0.3 3,194,151 - rfaE b3052 0.56 -1.9 3,194,213 + rfaE b3052 0.61 +1.5 3,194,221 - rfaE b3052 0.61 +0.7 3,194,300 - +1.3 3,194,300 - rfaE b3052 0.67 +0.6 3,194,300 - rfaE b3052 0.67 +1.7 3,194,396 - rfaE b3052 0.74 +4.7 3,194,430 + rfaE b3052 0.76 +1.9 3,194,495 - rfaE b3052 0.80 +0.5 3,194,556 - rfaE b3052 0.85 +1.6 3,194,556 - rfaE b3052 0.85 +1.0 3,194,585 - rfaE b3052 0.87 +2.4 3,194,618 - rfaE b3052 0.89 +1.2 3,194,631 - rfaE b3052 0.90 +1.4 3,194,768 + +0.0 3,194,768 + +0.5 3,194,790 - -0.6 3,194,821 - +0.0 3,194,821 - +0.2 3,194,824 - +0.2 3,194,954 + +0.8 3,194,958 - +0.9 3,194,958 - +0.0 3,194,964 - -0.1 3,194,964 - +0.5 3,194,992 - +0.3 3,195,011 + -0.1 3,195,011 + -0.7 3,195,069 + +0.1 3,195,130 + glnE b3053 0.11 +0.0 3,195,130 + glnE b3053 0.11 +1.2 3,195,130 + glnE b3053 0.11 +0.4 3,195,208 + glnE b3053 0.14 -0.0 3,195,208 + glnE b3053 0.14 +1.1 3,195,287 + glnE b3053 0.16 +0.3 3,195,385 + glnE b3053 0.20 +0.4 3,195,442 - glnE b3053 0.22 -0.1 3,195,442 - glnE b3053 0.22 +1.3 3,195,463 - glnE b3053 0.23 +0.9 3,195,478 + glnE b3053 0.23 +0.3 3,195,478 + glnE b3053 0.23 -0.9 3,195,479 + glnE b3053 0.23 -0.1 3,195,555 + glnE b3053 0.26 +0.1 3,195,581 - glnE b3053 0.27 -0.2 3,195,718 + glnE b3053 0.32 -0.1 3,195,731 - glnE b3053 0.32 -0.2
Or see this region's nucleotide sequence