Strain Fitness in Escherichia coli BW25113 around b2969

Experiment: RCH2_defined_1percent_glucose with Chloride 200 mM and Betaine 100 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyqgA and pheV are separated by 105 nucleotidespheV and yghD are separated by 148 nucleotidesyghD and yghE are separated by 1 nucleotidesyghE and yghF are separated by 2 nucleotidesyghF and yghG are separated by 125 nucleotides b2966: yqgA - predicted inner membrane protein (NCBI), at 3,107,575 to 3,108,282 yqgA b2967: pheV - tRNA-Phe (NCBI), at 3,108,388 to 3,108,463 pheV b2968: yghD - predicted secretion pathway M-type protein, membrane anchored (NCBI), at 3,108,612 to 3,109,148 yghD b2969: yghE - predicted secretion pathway protein, L-type protein (pseudogene) (RefSeq), at 3,109,150 to 3,110,112 yghE b2970: yghF - predicted secretion pathway protein, C-type protein (NCBI), at 3,110,115 to 3,110,963 yghF b2971: yghG - hypothetical protein (NCBI), at 3,111,089 to 3,111,499 yghG Position (kb) 3109 3110 3111Strain fitness (log2 ratio) -2 -1 0 1at 3108.160 kb on + strand, within yqgAat 3108.181 kb on - strand, within yqgAat 3108.221 kb on - strandat 3108.222 kb on + strandat 3108.222 kb on + strandat 3108.378 kb on - strandat 3108.378 kb on - strandat 3108.593 kb on + strandat 3108.629 kb on - strandat 3108.629 kb on - strandat 3108.649 kb on + strandat 3108.766 kb on + strand, within yghDat 3108.821 kb on + strand, within yghDat 3108.833 kb on - strand, within yghDat 3108.833 kb on - strand, within yghDat 3108.878 kb on + strand, within yghDat 3108.931 kb on - strand, within yghDat 3108.940 kb on + strand, within yghDat 3108.940 kb on + strand, within yghDat 3109.078 kb on - strand, within yghDat 3109.098 kb on - strandat 3109.123 kb on - strandat 3109.174 kb on + strandat 3109.220 kb on - strandat 3109.437 kb on + strand, within yghEat 3109.538 kb on + strand, within yghEat 3109.551 kb on + strand, within yghEat 3109.704 kb on + strand, within yghEat 3109.704 kb on + strand, within yghEat 3109.712 kb on - strand, within yghEat 3109.886 kb on - strand, within yghEat 3109.933 kb on - strand, within yghEat 3110.044 kb on - strandat 3110.073 kb on + strandat 3110.073 kb on + strandat 3110.085 kb on - strandat 3110.085 kb on - strandat 3110.184 kb on + strandat 3110.209 kb on - strand, within yghFat 3110.231 kb on - strand, within yghFat 3110.296 kb on - strand, within yghFat 3110.328 kb on + strand, within yghFat 3110.328 kb on + strand, within yghFat 3110.329 kb on - strand, within yghFat 3110.342 kb on - strand, within yghFat 3110.426 kb on + strand, within yghFat 3110.427 kb on + strand, within yghFat 3110.427 kb on + strand, within yghFat 3110.507 kb on + strand, within yghFat 3110.550 kb on - strand, within yghFat 3110.622 kb on - strand, within yghFat 3110.622 kb on - strand, within yghFat 3110.664 kb on + strand, within yghFat 3110.723 kb on + strand, within yghFat 3110.732 kb on + strand, within yghFat 3110.741 kb on + strand, within yghFat 3110.819 kb on + strand, within yghFat 3110.826 kb on - strand, within yghFat 3110.855 kb on + strand, within yghFat 3110.855 kb on + strand, within yghFat 3110.855 kb on + strand, within yghFat 3110.857 kb on + strand, within yghFat 3110.865 kb on - strand, within yghFat 3110.877 kb on + strand, within yghFat 3110.877 kb on + strand, within yghFat 3110.886 kb on + strandat 3110.886 kb on + strandat 3110.888 kb on + strandat 3110.888 kb on + strandat 3110.894 kb on - strandat 3110.935 kb on - strandat 3110.935 kb on - strandat 3110.977 kb on + strandat 3110.977 kb on + strandat 3110.986 kb on - strandat 3110.994 kb on + strandat 3110.996 kb on + strandat 3111.031 kb on - strandat 3111.031 kb on - strandat 3111.070 kb on + strandat 3111.086 kb on + strandat 3111.102 kb on + strandat 3111.103 kb on + strandat 3111.108 kb on - strandat 3111.108 kb on - strandat 3111.110 kb on - strandat 3111.110 kb on - strandat 3111.111 kb on - strandat 3111.111 kb on - strandat 3111.111 kb on - strandat 3111.111 kb on - strandat 3111.111 kb on - strandat 3111.111 kb on - strandat 3111.111 kb on - strandat 3111.111 kb on - strandat 3111.111 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_1percent_glucose with Chloride 200 mM and Betaine 100 uM
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3,108,160 + yqgA b2966 0.83 +0.2
3,108,181 - yqgA b2966 0.86 -0.9
3,108,221 - -1.2
3,108,222 + -0.3
3,108,222 + +0.9
3,108,378 - -1.5
3,108,378 - +0.6
3,108,593 + +0.3
3,108,629 - +0.4
3,108,629 - +0.1
3,108,649 + -1.4
3,108,766 + yghD b2968 0.29 -0.6
3,108,821 + yghD b2968 0.39 +0.4
3,108,833 - yghD b2968 0.41 +0.4
3,108,833 - yghD b2968 0.41 +1.3
3,108,878 + yghD b2968 0.50 -0.1
3,108,931 - yghD b2968 0.59 -0.8
3,108,940 + yghD b2968 0.61 +1.1
3,108,940 + yghD b2968 0.61 -0.4
3,109,078 - yghD b2968 0.87 -0.5
3,109,098 - -0.4
3,109,123 - +1.3
3,109,174 + -0.2
3,109,220 - +0.6
3,109,437 + yghE b2969 0.30 +0.6
3,109,538 + yghE b2969 0.40 +0.3
3,109,551 + yghE b2969 0.42 +0.5
3,109,704 + yghE b2969 0.58 +1.7
3,109,704 + yghE b2969 0.58 +0.5
3,109,712 - yghE b2969 0.58 +0.5
3,109,886 - yghE b2969 0.76 +0.4
3,109,933 - yghE b2969 0.81 -0.2
3,110,044 - -0.4
3,110,073 + +0.7
3,110,073 + -0.8
3,110,085 - +0.5
3,110,085 - -0.0
3,110,184 + -0.2
3,110,209 - yghF b2970 0.11 +0.6
3,110,231 - yghF b2970 0.14 +0.1
3,110,296 - yghF b2970 0.21 +0.3
3,110,328 + yghF b2970 0.25 +0.9
3,110,328 + yghF b2970 0.25 -0.4
3,110,329 - yghF b2970 0.25 +0.5
3,110,342 - yghF b2970 0.27 +0.5
3,110,426 + yghF b2970 0.37 -0.2
3,110,427 + yghF b2970 0.37 -0.6
3,110,427 + yghF b2970 0.37 -0.3
3,110,507 + yghF b2970 0.46 +0.1
3,110,550 - yghF b2970 0.51 +0.5
3,110,622 - yghF b2970 0.60 +0.2
3,110,622 - yghF b2970 0.60 -0.7
3,110,664 + yghF b2970 0.65 -0.9
3,110,723 + yghF b2970 0.72 -0.5
3,110,732 + yghF b2970 0.73 +0.1
3,110,741 + yghF b2970 0.74 +0.2
3,110,819 + yghF b2970 0.83 -0.4
3,110,826 - yghF b2970 0.84 +0.0
3,110,855 + yghF b2970 0.87 -0.3
3,110,855 + yghF b2970 0.87 +0.7
3,110,855 + yghF b2970 0.87 -2.3
3,110,857 + yghF b2970 0.87 +0.3
3,110,865 - yghF b2970 0.88 -1.0
3,110,877 + yghF b2970 0.90 +0.9
3,110,877 + yghF b2970 0.90 -0.6
3,110,886 + -0.1
3,110,886 + -0.1
3,110,888 + -0.7
3,110,888 + -0.7
3,110,894 - -0.0
3,110,935 - -0.7
3,110,935 - +0.4
3,110,977 + -0.8
3,110,977 + +0.1
3,110,986 - +0.3
3,110,994 + -0.7
3,110,996 + -1.5
3,111,031 - +0.5
3,111,031 - +0.1
3,111,070 + +0.5
3,111,086 + -0.9
3,111,102 + +0.2
3,111,103 + -1.3
3,111,108 - -0.5
3,111,108 - +0.1
3,111,110 - +1.3
3,111,110 - +0.8
3,111,111 - -1.4
3,111,111 - -0.5
3,111,111 - -0.2
3,111,111 - +0.2
3,111,111 - -0.2
3,111,111 - -1.2
3,111,111 - +1.7
3,111,111 - +0.3
3,111,111 - +0.1

Or see this region's nucleotide sequence