Strain Fitness in Escherichia coli BW25113 around b2050

Experiment: RCH2_defined_1percent_glucose with Chloride 200 mM and Betaine 100 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcpsB and wcaI are separated by 2 nucleotideswcaI and wcaH overlap by 4 nucleotideswcaH and fcl are separated by 2 nucleotides b2049: cpsB - mannose-1-phosphate guanyltransferase (NCBI), at 2,121,108 to 2,122,544 cpsB b2050: wcaI - predicted glycosyl transferase (NCBI), at 2,122,547 to 2,123,770 wcaI b2051: wcaH - GDP-mannose mannosyl hydrolase (VIMSS), at 2,123,767 to 2,124,246 wcaH b2052: fcl - bifunctional GDP-fucose synthetase: GDP-4-dehydro-6-deoxy-D-mannose epimerase/ GDP-4-dehydro-6-L-deoxygalactose reductase (NCBI), at 2,124,249 to 2,125,214 fcl Position (kb) 2122 2123 2124Strain fitness (log2 ratio) -3 -2 -1 0 1at 2121.605 kb on + strand, within cpsBat 2121.742 kb on + strand, within cpsBat 2121.742 kb on + strand, within cpsBat 2121.969 kb on + strand, within cpsBat 2122.061 kb on + strand, within cpsBat 2122.061 kb on + strand, within cpsBat 2122.748 kb on + strand, within wcaIat 2122.748 kb on + strand, within wcaIat 2123.290 kb on + strand, within wcaIat 2123.290 kb on + strand, within wcaIat 2123.479 kb on + strand, within wcaIat 2123.610 kb on + strand, within wcaIat 2123.668 kb on + strandat 2123.822 kb on + strand, within wcaHat 2124.177 kb on - strand, within wcaHat 2124.282 kb on + strandat 2124.282 kb on + strandat 2124.465 kb on + strand, within fclat 2124.601 kb on + strand, within fclat 2124.630 kb on + strand, within fclat 2124.630 kb on + strand, within fclat 2124.722 kb on - strand, within fcl

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_1percent_glucose with Chloride 200 mM and Betaine 100 uM
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2,121,605 + cpsB b2049 0.35 -0.7
2,121,742 + cpsB b2049 0.44 +0.0
2,121,742 + cpsB b2049 0.44 -0.6
2,121,969 + cpsB b2049 0.60 -1.4
2,122,061 + cpsB b2049 0.66 -0.3
2,122,061 + cpsB b2049 0.66 -0.9
2,122,748 + wcaI b2050 0.16 +0.7
2,122,748 + wcaI b2050 0.16 -0.2
2,123,290 + wcaI b2050 0.61 +1.2
2,123,290 + wcaI b2050 0.61 +0.5
2,123,479 + wcaI b2050 0.76 -0.1
2,123,610 + wcaI b2050 0.87 +0.2
2,123,668 + +0.6
2,123,822 + wcaH b2051 0.11 +0.4
2,124,177 - wcaH b2051 0.85 -1.2
2,124,282 + +0.2
2,124,282 + +0.4
2,124,465 + fcl b2052 0.22 -0.6
2,124,601 + fcl b2052 0.36 +0.2
2,124,630 + fcl b2052 0.39 -3.1
2,124,630 + fcl b2052 0.39 -0.3
2,124,722 - fcl b2052 0.49 -0.5

Or see this region's nucleotide sequence