Strain Fitness in Escherichia coli BW25113 around b1861

Experiment: RCH2_defined_1percent_glucose with Chloride 200 mM and Betaine 100 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntruvB and ruvA are separated by 8 nucleotidesruvA and yobI are separated by 138 nucleotidesyobI and yebB are separated by 70 nucleotidesyebB and ruvC are separated by 1 nucleotides b1860: ruvB - Holliday junction DNA helicase B (NCBI), at 1,942,370 to 1,943,380 ruvB b1861: ruvA - Holliday junction DNA helicase motor protein (NCBI), at 1,943,389 to 1,944,000 ruvA b4677: yobI - expressed protein (RefSeq), at 1,944,139 to 1,944,204 yobI b1862: yebB - orf, hypothetical protein (VIMSS), at 1,944,275 to 1,944,877 yebB b1863: ruvC - Holliday junction resolvase (NCBI), at 1,944,879 to 1,945,400 ruvC Position (kb) 1943 1944 1945Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1942.423 kb on + strandat 1942.445 kb on - strandat 1942.446 kb on - strandat 1942.806 kb on + strand, within ruvBat 1942.985 kb on + strand, within ruvBat 1943.892 kb on + strand, within ruvAat 1943.919 kb on - strand, within ruvAat 1943.934 kb on - strand, within ruvAat 1943.990 kb on - strandat 1943.996 kb on + strandat 1943.996 kb on + strandat 1944.021 kb on + strandat 1944.102 kb on + strandat 1944.102 kb on + strandat 1944.137 kb on + strandat 1944.333 kb on - strandat 1944.333 kb on - strandat 1944.436 kb on - strand, within yebBat 1944.511 kb on - strand, within yebBat 1944.560 kb on - strand, within yebBat 1944.582 kb on + strand, within yebBat 1944.622 kb on + strand, within yebBat 1944.625 kb on - strand, within yebBat 1944.625 kb on - strand, within yebBat 1944.625 kb on - strand, within yebBat 1944.669 kb on - strand, within yebBat 1944.674 kb on - strand, within yebBat 1944.723 kb on - strand, within yebBat 1944.818 kb on + strandat 1944.818 kb on + strandat 1944.826 kb on - strandat 1944.826 kb on - strandat 1944.829 kb on + strandat 1944.846 kb on - strandat 1944.885 kb on - strandat 1944.885 kb on - strandat 1944.895 kb on - strandat 1944.908 kb on + strandat 1944.916 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_1percent_glucose with Chloride 200 mM and Betaine 100 uM
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1,942,423 + -1.6
1,942,445 - +0.0
1,942,446 - -2.7
1,942,806 + ruvB b1860 0.43 +2.4
1,942,985 + ruvB b1860 0.61 +0.3
1,943,892 + ruvA b1861 0.82 -0.5
1,943,919 - ruvA b1861 0.87 +3.3
1,943,934 - ruvA b1861 0.89 -0.7
1,943,990 - -1.3
1,943,996 + -1.7
1,943,996 + -0.3
1,944,021 + +1.4
1,944,102 + +2.3
1,944,102 + -0.1
1,944,137 + +0.3
1,944,333 - +1.2
1,944,333 - +0.4
1,944,436 - yebB b1862 0.27 +0.3
1,944,511 - yebB b1862 0.39 +1.3
1,944,560 - yebB b1862 0.47 -0.5
1,944,582 + yebB b1862 0.51 +1.3
1,944,622 + yebB b1862 0.58 +1.3
1,944,625 - yebB b1862 0.58 -1.5
1,944,625 - yebB b1862 0.58 +1.2
1,944,625 - yebB b1862 0.58 +0.7
1,944,669 - yebB b1862 0.65 -1.3
1,944,674 - yebB b1862 0.66 +1.4
1,944,723 - yebB b1862 0.74 -0.7
1,944,818 + +2.8
1,944,818 + +1.3
1,944,826 - -0.1
1,944,826 - +1.3
1,944,829 + +0.3
1,944,846 - -1.9
1,944,885 - +0.5
1,944,885 - +0.4
1,944,895 - +0.0
1,944,908 + +1.3
1,944,916 - +0.0

Or see this region's nucleotide sequence