Strain Fitness in Escherichia coli BW25113 around b0885

Experiment: RCH2_defined_1percent_glucose with Chloride 200 mM and Betaine 100 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntserW and infA are separated by 253 nucleotidesinfA and aat are separated by 284 nucleotidesaat and cydC are separated by 41 nucleotides b0883: serW - tRNA-Ser (NCBI), at 925,107 to 925,194 serW b0884: infA - translation initiation factor IF-1 (NCBI), at 925,448 to 925,666 infA b0885: aat - leucyl/phenylalanyl-tRNA--protein transferase (NCBI), at 925,951 to 926,655 aat b0886: cydC - fused cysteine transporter subunits of ABC superfamily: membrane component/ATP-binding component (NCBI), at 926,697 to 928,418 cydC Position (kb) 925 926 927Strain fitness (log2 ratio) -1 0 1at 924.954 kb on + strandat 925.246 kb on - strandat 925.246 kb on - strandat 925.319 kb on + strandat 925.319 kb on + strandat 925.319 kb on + strandat 925.327 kb on - strandat 925.686 kb on - strandat 925.687 kb on - strandat 925.687 kb on - strandat 925.779 kb on - strandat 925.885 kb on + strandat 925.967 kb on + strandat 925.967 kb on + strandat 926.089 kb on + strand, within aatat 926.206 kb on + strand, within aatat 926.258 kb on + strand, within aatat 926.299 kb on + strand, within aatat 926.361 kb on - strand, within aatat 926.423 kb on + strand, within aatat 926.453 kb on - strand, within aatat 926.479 kb on + strand, within aatat 926.693 kb on - strandat 926.717 kb on + strandat 926.730 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_1percent_glucose with Chloride 200 mM and Betaine 100 uM
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924,954 + +0.0
925,246 - +0.8
925,246 - -0.0
925,319 + +0.1
925,319 + -0.3
925,319 + +0.3
925,327 - -0.4
925,686 - +1.3
925,687 - +1.1
925,687 - +0.7
925,779 - -0.4
925,885 + +0.5
925,967 + -0.0
925,967 + -0.8
926,089 + aat b0885 0.20 +0.6
926,206 + aat b0885 0.36 +0.6
926,258 + aat b0885 0.44 +0.8
926,299 + aat b0885 0.49 +0.1
926,361 - aat b0885 0.58 +0.5
926,423 + aat b0885 0.67 +0.4
926,453 - aat b0885 0.71 -0.5
926,479 + aat b0885 0.75 +0.8
926,693 - -1.3
926,717 + -0.0
926,730 - -0.4

Or see this region's nucleotide sequence