Strain Fitness in Escherichia coli BW25113 around b0018

Experiment: RCH2_defined_1percent_glucose with Chloride 200 mM and Betaine 100 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntinsL-1 and mokC are separated by 193 nucleotidesmokC and hokC overlap by 210 nucleotideshokC and sokC are separated by 48 nucleotidessokC and nhaA are separated by 482 nucleotides b0016: insL-1 - IS186/IS421 transposase (NCBI), at 15,445 to 16,557 insL-1 b0018: mokC - regulatory protein for HokC, overlaps CDS of hokC (NCBI), at 16,751 to 16,960 mokC b4412: hokC - toxic membrane protein, small (NCBI), at 16,751 to 16,903 hokC b4413: sokC - regulatory antisense RNA (NCBI), at 16,952 to 17,006 sokC b0019: nhaA - pH-dependent sodium/proton antiporter (NCBI), at 17,489 to 18,655 nhaA Position (kb) 16 17Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4at 16.703 kb on + strandat 16.729 kb on + strandat 16.729 kb on + strandat 16.895 kb on - strandat 16.895 kb on - strandat 16.920 kb on + strand, within mokCat 16.942 kb on + strandat 16.950 kb on - strandat 16.950 kb on - strandat 16.950 kb on - strandat 16.950 kb on - strandat 16.950 kb on - strandat 16.950 kb on - strandat 16.955 kb on - strandat 16.957 kb on + strandat 16.957 kb on + strandat 16.957 kb on + strandat 16.976 kb on - strand, within sokCat 17.024 kb on - strandat 17.032 kb on + strandat 17.037 kb on + strandat 17.037 kb on + strandat 17.058 kb on + strandat 17.058 kb on + strandat 17.058 kb on + strandat 17.060 kb on + strandat 17.060 kb on + strandat 17.096 kb on + strandat 17.097 kb on + strandat 17.104 kb on + strandat 17.104 kb on + strandat 17.112 kb on - strandat 17.306 kb on + strandat 17.318 kb on - strandat 17.330 kb on - strandat 17.339 kb on - strandat 17.339 kb on - strandat 17.503 kb on - strandat 17.517 kb on - strandat 17.517 kb on - strandat 17.517 kb on - strandat 17.537 kb on + strandat 17.555 kb on + strandat 17.581 kb on - strandat 17.600 kb on + strandat 17.621 kb on + strand, within nhaAat 17.671 kb on + strand, within nhaAat 17.676 kb on - strandat 17.749 kb on - strand, within nhaAat 17.749 kb on - strand, within nhaAat 17.765 kb on - strand, within nhaAat 17.765 kb on - strand, within nhaAat 17.813 kb on + strand, within nhaAat 17.879 kb on + strand, within nhaAat 17.887 kb on - strand, within nhaAat 17.902 kb on - strand, within nhaAat 17.904 kb on + strand, within nhaAat 17.917 kb on + strand, within nhaAat 17.925 kb on - strand, within nhaAat 17.925 kb on - strand, within nhaAat 17.925 kb on - strand, within nhaA

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_1percent_glucose with Chloride 200 mM and Betaine 100 uM
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16,703 + +0.5
16,729 + -2.5
16,729 + -0.4
16,895 - +0.3
16,895 - +0.0
16,920 + mokC b0018 0.80 +1.0
16,942 + -0.4
16,950 - -2.3
16,950 - -0.1
16,950 - +0.8
16,950 - +0.2
16,950 - +0.8
16,950 - +0.7
16,955 - +1.3
16,957 + -0.8
16,957 + +1.2
16,957 + -0.6
16,976 - sokC b4413 0.44 -0.2
17,024 - +0.1
17,032 + +0.7
17,037 + +1.5
17,037 + +1.7
17,058 + +1.1
17,058 + +0.7
17,058 + +1.5
17,060 + +2.4
17,060 + -2.8
17,096 + -0.5
17,097 + -0.2
17,104 + +1.1
17,104 + +4.2
17,112 - -1.6
17,306 + +1.8
17,318 - -0.9
17,330 - -2.0
17,339 - -0.4
17,339 - -0.7
17,503 - -4.3
17,517 - -1.5
17,517 - -3.2
17,517 - -0.3
17,537 + -3.4
17,555 + -3.1
17,581 - -3.0
17,600 + -1.8
17,621 + nhaA b0019 0.11 -1.7
17,671 + nhaA b0019 0.16 -2.0
17,676 - -0.5
17,749 - nhaA b0019 0.22 -2.1
17,749 - nhaA b0019 0.22 -4.0
17,765 - nhaA b0019 0.24 -2.7
17,765 - nhaA b0019 0.24 -3.9
17,813 + nhaA b0019 0.28 -3.6
17,879 + nhaA b0019 0.33 -4.1
17,887 - nhaA b0019 0.34 -3.9
17,902 - nhaA b0019 0.35 -4.3
17,904 + nhaA b0019 0.36 -0.5
17,917 + nhaA b0019 0.37 -3.9
17,925 - nhaA b0019 0.37 -1.3
17,925 - nhaA b0019 0.37 -1.8
17,925 - nhaA b0019 0.37 -2.7

Or see this region's nucleotide sequence