Strain Fitness in Escherichia coli BW25113 around b4224

Experiment: RCH2_defined_1percent_glucose with Chloride 100 mM and Betaine 100 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntytfN and ytfP are separated by 2 nucleotidesytfP and chpS are separated by 211 nucleotideschpS and chpB overlap by 7 nucleotideschpB and ppa are separated by 79 nucleotides b4221: ytfN - hypothetical protein (NCBI), at 4,442,135 to 4,445,914 ytfN b4222: ytfP - hypothetical protein (NCBI), at 4,445,917 to 4,446,258 ytfP b4224: chpS - suppressor of inhibitory function of ChpB, PemI-like, autoregulated (VIMSS), at 4,446,470 to 4,446,721 chpS b4225: chpB - toxin of the ChpB-ChpS toxin-antitoxin system (NCBI), at 4,446,715 to 4,447,065 chpB b4226: ppa - inorganic pyrophosphatase (NCBI), at 4,447,145 to 4,447,675 ppa Position (kb) 4446 4447Strain fitness (log2 ratio) -1 0 1 2at 4445.535 kb on - strand, within ytfNat 4445.639 kb on + strandat 4445.668 kb on - strandat 4445.668 kb on - strandat 4445.736 kb on + strandat 4445.737 kb on + strandat 4445.737 kb on + strandat 4445.770 kb on - strandat 4445.794 kb on + strandat 4445.806 kb on + strandat 4445.806 kb on + strandat 4445.806 kb on + strandat 4445.836 kb on + strandat 4445.836 kb on + strandat 4445.859 kb on - strandat 4445.866 kb on + strandat 4445.866 kb on + strandat 4445.962 kb on + strand, within ytfPat 4445.962 kb on + strand, within ytfPat 4446.046 kb on - strand, within ytfPat 4446.131 kb on + strand, within ytfPat 4446.166 kb on + strand, within ytfPat 4446.166 kb on + strand, within ytfPat 4446.166 kb on + strand, within ytfPat 4446.176 kb on + strand, within ytfPat 4446.176 kb on - strand, within ytfPat 4446.176 kb on - strand, within ytfPat 4446.176 kb on - strand, within ytfPat 4446.184 kb on - strand, within ytfPat 4446.184 kb on - strand, within ytfPat 4446.240 kb on + strandat 4446.259 kb on + strandat 4446.260 kb on + strandat 4446.272 kb on - strandat 4446.273 kb on - strandat 4446.273 kb on - strandat 4446.375 kb on - strandat 4446.454 kb on + strandat 4446.454 kb on + strandat 4446.496 kb on + strand, within chpSat 4446.501 kb on + strand, within chpSat 4446.501 kb on + strand, within chpSat 4446.509 kb on - strand, within chpSat 4446.509 kb on - strand, within chpSat 4446.514 kb on - strand, within chpSat 4446.559 kb on + strand, within chpSat 4446.569 kb on - strand, within chpSat 4446.569 kb on - strand, within chpSat 4446.646 kb on - strand, within chpSat 4446.652 kb on - strand, within chpSat 4446.746 kb on + strandat 4446.777 kb on + strand, within chpBat 4446.787 kb on + strand, within chpBat 4446.787 kb on + strand, within chpBat 4446.787 kb on - strand, within chpBat 4446.787 kb on - strand, within chpBat 4446.794 kb on + strand, within chpBat 4446.910 kb on + strand, within chpBat 4446.916 kb on - strand, within chpBat 4446.945 kb on + strand, within chpBat 4446.949 kb on - strand, within chpBat 4446.960 kb on + strand, within chpBat 4447.006 kb on - strand, within chpBat 4447.006 kb on - strand, within chpBat 4447.057 kb on + strandat 4447.060 kb on + strandat 4447.060 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_1percent_glucose with Chloride 100 mM and Betaine 100 uM
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4,445,535 - ytfN b4221 0.90 +0.6
4,445,639 + -0.5
4,445,668 - +0.7
4,445,668 - -0.9
4,445,736 + -0.6
4,445,737 + -1.5
4,445,737 + +0.6
4,445,770 - +0.9
4,445,794 + +0.6
4,445,806 + -0.1
4,445,806 + +0.3
4,445,806 + +0.1
4,445,836 + -0.6
4,445,836 + -0.6
4,445,859 - -0.0
4,445,866 + +0.4
4,445,866 + -0.1
4,445,962 + ytfP b4222 0.13 +0.1
4,445,962 + ytfP b4222 0.13 -0.2
4,446,046 - ytfP b4222 0.38 -0.8
4,446,131 + ytfP b4222 0.63 +1.1
4,446,166 + ytfP b4222 0.73 +0.4
4,446,166 + ytfP b4222 0.73 -0.9
4,446,166 + ytfP b4222 0.73 +0.3
4,446,176 + ytfP b4222 0.76 -0.7
4,446,176 - ytfP b4222 0.76 -0.1
4,446,176 - ytfP b4222 0.76 +0.2
4,446,176 - ytfP b4222 0.76 -1.3
4,446,184 - ytfP b4222 0.78 +0.7
4,446,184 - ytfP b4222 0.78 -0.3
4,446,240 + -0.3
4,446,259 + -0.2
4,446,260 + +0.2
4,446,272 - -0.6
4,446,273 - +1.0
4,446,273 - -1.2
4,446,375 - +0.9
4,446,454 + -0.9
4,446,454 + +0.2
4,446,496 + chpS b4224 0.10 +0.2
4,446,501 + chpS b4224 0.12 +0.4
4,446,501 + chpS b4224 0.12 +0.5
4,446,509 - chpS b4224 0.15 -0.2
4,446,509 - chpS b4224 0.15 +0.5
4,446,514 - chpS b4224 0.17 +0.2
4,446,559 + chpS b4224 0.35 +1.9
4,446,569 - chpS b4224 0.39 -0.4
4,446,569 - chpS b4224 0.39 +1.3
4,446,646 - chpS b4224 0.70 -0.2
4,446,652 - chpS b4224 0.72 +0.1
4,446,746 + -0.1
4,446,777 + chpB b4225 0.18 -0.2
4,446,787 + chpB b4225 0.21 -0.2
4,446,787 + chpB b4225 0.21 -0.4
4,446,787 - chpB b4225 0.21 +0.6
4,446,787 - chpB b4225 0.21 -0.6
4,446,794 + chpB b4225 0.23 +0.1
4,446,910 + chpB b4225 0.56 -0.7
4,446,916 - chpB b4225 0.57 -0.2
4,446,945 + chpB b4225 0.66 -0.7
4,446,949 - chpB b4225 0.67 -0.0
4,446,960 + chpB b4225 0.70 +0.0
4,447,006 - chpB b4225 0.83 +0.2
4,447,006 - chpB b4225 0.83 -0.2
4,447,057 + +0.1
4,447,060 + +0.1
4,447,060 + +0.6

Or see this region's nucleotide sequence