Experiment: RCH2_defined_1percent_glucose with Chloride 100 mM and Betaine 100 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yiaF and yiaG are separated by 433 nucleotides yiaG and cspA are separated by 280 nucleotides cspA and hokA are separated by 186 nucleotides hokA and mokA overlap by 153 nucleotides mokA and insJ are separated by 47 nucleotides
b3554: yiaF - orf, hypothetical protein (VIMSS), at 3,716,357 to 3,717,067
yiaF
b3555: yiaG - predicted transcriptional regulator (NCBI), at 3,717,501 to 3,717,791
yiaG
b3556: cspA - major cold shock protein (NCBI), at 3,718,072 to 3,718,284
cspA
b4455: hokA - toxic polypeptide, small (NCBI), at 3,718,471 to 3,718,623
hokA
b4647: mokA - no description, at 3,718,471 to 3,718,655
mokA
b3557: insJ - IS150 protein InsA (NCBI), at 3,718,703 to 3,719,224
insJ
Position (kb)
3717
3718 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3716.502 kb on - strand, within yiaF at 3716.502 kb on - strand, within yiaF at 3716.504 kb on + strand, within yiaF at 3716.504 kb on + strand, within yiaF at 3716.509 kb on + strand, within yiaF at 3716.509 kb on + strand, within yiaF at 3716.514 kb on + strand, within yiaF at 3716.514 kb on + strand, within yiaF at 3716.522 kb on - strand, within yiaF at 3716.522 kb on - strand, within yiaF at 3716.522 kb on - strand, within yiaF at 3716.540 kb on - strand, within yiaF at 3716.594 kb on + strand, within yiaF at 3716.600 kb on - strand, within yiaF at 3716.618 kb on + strand, within yiaF at 3716.660 kb on - strand, within yiaF at 3716.695 kb on + strand, within yiaF at 3716.695 kb on + strand, within yiaF at 3716.710 kb on - strand, within yiaF at 3716.744 kb on + strand, within yiaF at 3716.786 kb on - strand, within yiaF at 3716.789 kb on + strand, within yiaF at 3716.797 kb on - strand, within yiaF at 3716.797 kb on - strand, within yiaF at 3716.841 kb on + strand, within yiaF at 3716.869 kb on - strand, within yiaF at 3716.905 kb on + strand, within yiaF at 3716.920 kb on - strand, within yiaF at 3716.920 kb on - strand, within yiaF at 3716.934 kb on + strand, within yiaF at 3716.942 kb on - strand, within yiaF at 3716.959 kb on - strand, within yiaF at 3716.959 kb on - strand, within yiaF at 3716.966 kb on + strand, within yiaF at 3716.988 kb on - strand, within yiaF at 3717.001 kb on + strand at 3717.001 kb on + strand at 3717.085 kb on - strand at 3717.085 kb on - strand at 3717.133 kb on + strand at 3717.133 kb on + strand at 3717.133 kb on + strand at 3717.133 kb on + strand at 3717.133 kb on + strand at 3717.136 kb on - strand at 3717.141 kb on - strand at 3717.141 kb on - strand at 3717.141 kb on - strand at 3717.141 kb on - strand at 3717.143 kb on + strand at 3717.151 kb on - strand at 3717.151 kb on - strand at 3717.264 kb on + strand at 3717.264 kb on + strand at 3717.322 kb on - strand at 3717.325 kb on - strand at 3717.362 kb on - strand at 3717.369 kb on - strand at 3717.407 kb on - strand at 3717.444 kb on + strand at 3717.475 kb on - strand at 3717.480 kb on - strand at 3717.480 kb on - strand at 3717.484 kb on + strand at 3717.536 kb on - strand, within yiaG at 3717.536 kb on - strand, within yiaG at 3717.536 kb on - strand, within yiaG at 3717.602 kb on - strand, within yiaG at 3717.602 kb on - strand, within yiaG at 3717.786 kb on + strand at 3717.805 kb on + strand at 3717.908 kb on + strand at 3717.914 kb on - strand at 3718.170 kb on + strand at 3718.267 kb on + strand at 3718.419 kb on + strand at 3718.476 kb on - strand at 3718.506 kb on + strand at 3718.507 kb on + strand at 3718.507 kb on + strand at 3718.507 kb on + strand at 3718.507 kb on + strand at 3718.514 kb on - strand at 3718.514 kb on - strand at 3718.514 kb on - strand at 3718.515 kb on - strand at 3718.515 kb on - strand at 3718.515 kb on - strand at 3718.516 kb on - strand at 3718.555 kb on - strand at 3718.555 kb on - strand at 3718.567 kb on + strand at 3718.624 kb on + strand, within mokA at 3718.624 kb on + strand, within mokA at 3718.636 kb on + strand, within mokA at 3718.636 kb on + strand, within mokA at 3718.638 kb on + strand at 3718.652 kb on + strand at 3718.652 kb on + strand at 3718.652 kb on + strand at 3718.667 kb on + strand at 3718.668 kb on + strand at 3718.756 kb on + strand, within insJ at 3718.756 kb on + strand, within insJ at 3718.763 kb on + strand, within insJ
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_1percent_glucose with Chloride 100 mM and Betaine 100 uM remove 3,716,502 - yiaF b3554 0.20 -0.0 3,716,502 - yiaF b3554 0.20 +0.3 3,716,504 + yiaF b3554 0.21 -0.7 3,716,504 + yiaF b3554 0.21 -1.5 3,716,509 + yiaF b3554 0.21 -0.3 3,716,509 + yiaF b3554 0.21 +1.2 3,716,514 + yiaF b3554 0.22 +0.2 3,716,514 + yiaF b3554 0.22 +0.1 3,716,522 - yiaF b3554 0.23 -0.1 3,716,522 - yiaF b3554 0.23 -0.3 3,716,522 - yiaF b3554 0.23 -0.7 3,716,540 - yiaF b3554 0.26 +0.0 3,716,594 + yiaF b3554 0.33 -0.1 3,716,600 - yiaF b3554 0.34 -0.2 3,716,618 + yiaF b3554 0.37 -0.3 3,716,660 - yiaF b3554 0.43 -0.0 3,716,695 + yiaF b3554 0.48 -0.2 3,716,695 + yiaF b3554 0.48 -0.8 3,716,710 - yiaF b3554 0.50 -1.0 3,716,744 + yiaF b3554 0.54 +0.8 3,716,786 - yiaF b3554 0.60 -0.3 3,716,789 + yiaF b3554 0.61 -0.6 3,716,797 - yiaF b3554 0.62 -0.7 3,716,797 - yiaF b3554 0.62 +0.3 3,716,841 + yiaF b3554 0.68 -0.2 3,716,869 - yiaF b3554 0.72 -0.9 3,716,905 + yiaF b3554 0.77 +0.4 3,716,920 - yiaF b3554 0.79 +0.8 3,716,920 - yiaF b3554 0.79 -0.4 3,716,934 + yiaF b3554 0.81 +0.1 3,716,942 - yiaF b3554 0.82 +0.5 3,716,959 - yiaF b3554 0.85 +0.4 3,716,959 - yiaF b3554 0.85 +1.7 3,716,966 + yiaF b3554 0.86 +1.0 3,716,988 - yiaF b3554 0.89 -0.0 3,717,001 + -0.2 3,717,001 + -0.1 3,717,085 - +0.7 3,717,085 - +1.0 3,717,133 + -0.1 3,717,133 + -0.8 3,717,133 + -0.3 3,717,133 + +0.5 3,717,133 + +0.1 3,717,136 - +0.4 3,717,141 - +0.0 3,717,141 - -0.0 3,717,141 - -0.2 3,717,141 - +0.3 3,717,143 + +0.5 3,717,151 - +0.2 3,717,151 - +0.9 3,717,264 + -0.0 3,717,264 + -0.1 3,717,322 - +0.2 3,717,325 - -0.7 3,717,362 - -0.1 3,717,369 - +0.2 3,717,407 - -0.4 3,717,444 + +0.7 3,717,475 - +0.9 3,717,480 - -0.4 3,717,480 - -0.5 3,717,484 + +0.6 3,717,536 - yiaG b3555 0.12 +1.0 3,717,536 - yiaG b3555 0.12 +0.1 3,717,536 - yiaG b3555 0.12 +0.3 3,717,602 - yiaG b3555 0.35 +0.3 3,717,602 - yiaG b3555 0.35 +0.7 3,717,786 + -1.9 3,717,805 + +0.5 3,717,908 + +1.2 3,717,914 - -0.2 3,718,170 + +2.9 3,718,267 + +0.7 3,718,419 + +0.3 3,718,476 - +0.2 3,718,506 + -0.1 3,718,507 + +1.9 3,718,507 + +1.9 3,718,507 + -0.3 3,718,507 + +1.9 3,718,514 - +0.2 3,718,514 - +0.5 3,718,514 - -0.2 3,718,515 - -0.5 3,718,515 - -0.4 3,718,515 - -1.1 3,718,516 - +0.3 3,718,555 - -0.0 3,718,555 - +0.2 3,718,567 + +0.9 3,718,624 + mokA b4647 0.83 +3.5 3,718,624 + mokA b4647 0.83 -1.4 3,718,636 + mokA b4647 0.89 -0.4 3,718,636 + mokA b4647 0.89 -1.1 3,718,638 + -0.4 3,718,652 + +2.4 3,718,652 + -0.4 3,718,652 + -0.1 3,718,667 + +0.9 3,718,668 + +0.4 3,718,756 + insJ b3557 0.10 -1.1 3,718,756 + insJ b3557 0.10 +0.3 3,718,763 + insJ b3557 0.11 +2.1
Or see this region's nucleotide sequence