Experiment: RCH2_defined_1percent_glucose with Chloride 100 mM and Betaine 100 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yegQ and cyaR are separated by 84 nucleotides cyaR and ogrK are separated by 101 nucleotides ogrK and yegZ are separated by 81 nucleotides yegZ and b2085 are separated by 174 nucleotides b2085 and yegS are separated by 405 nucleotides
b2081: yegQ - predicted peptidase (NCBI), at 2,163,692 to 2,165,053
yegQ
b4438: cyaR - sRNA effector of ompX mRNA instability, cAMP-induced; hfq-dependent (RefSeq), at 2,165,138 to 2,165,224
cyaR
b2082: ogrK - DNA-binding transcriptional regulator prophage P2 remnant (NCBI), at 2,165,326 to 2,165,544
ogrK
b2083: yegZ - orf, hypothetical protein (VIMSS), at 2,165,626 to 2,165,838
yegZ
b2085: b2085 - orf, hypothetical protein (VIMSS), at 2,166,013 to 2,166,330
b2085
b2086: yegS - hypothetical protein (NCBI), at 2,166,736 to 2,167,635
yegS
Position (kb)
2165
2166 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2164.653 kb on + strand, within yegQ at 2164.657 kb on + strand, within yegQ at 2164.713 kb on + strand, within yegQ at 2164.713 kb on + strand, within yegQ at 2164.798 kb on + strand, within yegQ at 2164.927 kb on - strand at 2164.950 kb on + strand at 2165.031 kb on + strand at 2165.109 kb on - strand at 2165.109 kb on - strand at 2165.177 kb on - strand, within cyaR at 2165.184 kb on + strand, within cyaR at 2165.184 kb on + strand, within cyaR at 2165.383 kb on - strand, within ogrK at 2165.423 kb on - strand, within ogrK at 2165.658 kb on - strand, within yegZ at 2165.732 kb on + strand, within yegZ at 2165.732 kb on + strand, within yegZ at 2165.872 kb on + strand at 2165.894 kb on - strand at 2165.907 kb on + strand at 2165.929 kb on + strand at 2165.929 kb on + strand at 2165.964 kb on + strand at 2165.968 kb on + strand at 2166.018 kb on + strand at 2166.018 kb on + strand at 2166.023 kb on + strand at 2166.047 kb on + strand, within b2085 at 2166.047 kb on + strand, within b2085 at 2166.047 kb on - strand, within b2085 at 2166.047 kb on - strand, within b2085 at 2166.052 kb on - strand, within b2085 at 2166.054 kb on + strand, within b2085 at 2166.054 kb on + strand, within b2085 at 2166.054 kb on + strand, within b2085 at 2166.054 kb on + strand, within b2085 at 2166.054 kb on + strand, within b2085 at 2166.054 kb on + strand, within b2085 at 2166.055 kb on + strand, within b2085 at 2166.062 kb on - strand, within b2085 at 2166.102 kb on + strand, within b2085 at 2166.102 kb on + strand, within b2085 at 2166.155 kb on + strand, within b2085 at 2166.188 kb on + strand, within b2085 at 2166.188 kb on + strand, within b2085 at 2166.588 kb on - strand at 2166.634 kb on - strand at 2166.634 kb on - strand at 2166.635 kb on - strand at 2166.636 kb on - strand at 2166.661 kb on - strand at 2166.693 kb on + strand at 2166.693 kb on + strand at 2166.698 kb on + strand at 2166.708 kb on - strand at 2166.713 kb on - strand at 2166.723 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_1percent_glucose with Chloride 100 mM and Betaine 100 uM remove 2,164,653 + yegQ b2081 0.71 -0.2 2,164,657 + yegQ b2081 0.71 +0.2 2,164,713 + yegQ b2081 0.75 -0.3 2,164,713 + yegQ b2081 0.75 -0.3 2,164,798 + yegQ b2081 0.81 -0.1 2,164,927 - -1.0 2,164,950 + +0.6 2,165,031 + -1.2 2,165,109 - -1.0 2,165,109 - +0.1 2,165,177 - cyaR b4438 0.45 -1.6 2,165,184 + cyaR b4438 0.53 +0.3 2,165,184 + cyaR b4438 0.53 +1.0 2,165,383 - ogrK b2082 0.26 +0.3 2,165,423 - ogrK b2082 0.44 -0.1 2,165,658 - yegZ b2083 0.15 +1.5 2,165,732 + yegZ b2083 0.50 +0.3 2,165,732 + yegZ b2083 0.50 +0.0 2,165,872 + +0.1 2,165,894 - +0.3 2,165,907 + -0.2 2,165,929 + -0.7 2,165,929 + -0.0 2,165,964 + -0.2 2,165,968 + -0.4 2,166,018 + -0.7 2,166,018 + +0.9 2,166,023 + +0.6 2,166,047 + b2085 b2085 0.11 -1.2 2,166,047 + b2085 b2085 0.11 +0.7 2,166,047 - b2085 b2085 0.11 +1.2 2,166,047 - b2085 b2085 0.11 -0.8 2,166,052 - b2085 b2085 0.12 +1.9 2,166,054 + b2085 b2085 0.13 +0.4 2,166,054 + b2085 b2085 0.13 -0.3 2,166,054 + b2085 b2085 0.13 -0.7 2,166,054 + b2085 b2085 0.13 -0.4 2,166,054 + b2085 b2085 0.13 -0.1 2,166,054 + b2085 b2085 0.13 +0.4 2,166,055 + b2085 b2085 0.13 -0.8 2,166,062 - b2085 b2085 0.15 -0.4 2,166,102 + b2085 b2085 0.28 +0.6 2,166,102 + b2085 b2085 0.28 +0.1 2,166,155 + b2085 b2085 0.45 -0.4 2,166,188 + b2085 b2085 0.55 +1.2 2,166,188 + b2085 b2085 0.55 +0.2 2,166,588 - +0.2 2,166,634 - -1.5 2,166,634 - +1.2 2,166,635 - -1.8 2,166,636 - -0.8 2,166,661 - +1.4 2,166,693 + -1.4 2,166,693 + +0.2 2,166,698 + +0.2 2,166,708 - -0.0 2,166,713 - -0.4 2,166,723 - +0.3
Or see this region's nucleotide sequence